READ ME File For 'Load of motility-related microglial proteins, inflammation-related proteins, and microglial morphological features in controls, Alzheimer's Disease (AD) cases, and AD cases after Aß42-immunotherapy' Dataset DOI: 10.5258/SOTON/D1438 ReadMe Author: Diana Karina Franco Bocanegra, University of Southampton This dataset supports the thesis entitled 'Microglial Motility and Morphology in Alzheimer's Disease, and after Aß42-immunotherapy" AWARDED BY: University of Southampton DATE OF AWARD: 2020 DESCRIPTION OF THE DATA This dataset is divided in 11 sheets; the first eight sheets contain the results of the measurement of protein load of four different microglial motility-related proteins (Iba1, CFL1, CORO1A and P2RY12), in the grey matter (gm) and the white matter (wm) of human inferior parietal lobule slices, from three different study groups: controls (aged cases with no neurological disease, n=32), AD cases (n=44), and AD cases that received treatment with Aß42-immunotherapy (n=16; total n = 92 cases). The data on protein load was collected with the software ImageJ, using 30 images per case, after the slices were stained against the target proteins by DAB-immunohistochemistry.The ninth sheet (MSD) contains the results of the measurement of 20 different inflammation-related proteins, on frozen brain tissue of a subsample of 77 cases. These proteins were measured by Meso Scale Discovery multiplex commercial kits. The tenth sheet (2D morphology) contains the results of the assessment of microglial morphology of all cases, which was done on images of Iba1-stained grey matter tissue, by counting the number of microglial cells in the images and classifying them into three different morphological categories termed "ramified", "reactive" and "amoeboid". The eleventh and last sheet (3D morphology) contains the results of a detailed three-dimensional assessment of microglial morphological features, carried out in a subsample of 35 cases. Slices of brain tissue from these cases were stained with fluorescent immunohistochemistry and imaged in a confocal microscope. Individual microglial cells were 3D-reconstructed and morphological features were measured in the software Imaris. All data were statistically analysed in the software SPSS. No specialised software is necessary to access the data Date of data collection: January 2017 - January 2020 Related projects/Funders: This PhD studentship was funded by a Mexican government "CONACyT" scholarship (270152 / 438262), and a University of Southampton scholarship (VCFoM/BocheD/TBC/170). Alzheimer’s Research UK (ART/PG2006/4, ART-EXT2010–1, ARUK-EG2015A-4) supported the collection of the immunised AD cases. Related publication: Franco-Bocanegra, D.K., George, B., Lau, L.C. et al. Microglial motility in Alzheimer’s disease and after Aß42 immunotherapy: a human post-mortem study. acta neuropathol commun 7, 174 (2019). https://doi.org/10.1186/s40478-019-0828-x Date that the file was created: June 2020