Engineering functional nanoparticles for delivery in cells
Engineering functional nanoparticles for delivery in cells
The ability of DNA functionalised gold nanoparticles (AuNPs) to detect specific targets in vitro and in vivo has led to their development as suitable tools for sensing applications. However, endosomal entrapment is a common barrier in various nanoparticle delivery approaches. In this work, we present a new design strategy with the aim to enhance endosomal escape of DNA-coated AuNPs via the incorporation of a peptide that has been found to promote effective escape within cells. AuNPs are firstly modified with thiol terminated DNA strands followed by further surface functionalisation with cysteine terminated peptides. We show that optimized loading of peptides following DNA nanoparticle functionalisation of nanoparticles is feasible. DNA-peptide-coated AuNP hybrids show similar stability towards degradation by endocellular enzymes and similar specificity towards the detection of specific mRNA targets.
DNA, cells, mRNA detection, nanoparticles, peptide
Alexaki, Konstantina
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Kyriazi, Maria Eleni
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El Sagheer, Afaf H.
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Brown, Tom
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Kanaras, Antonios G.
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2020
Alexaki, Konstantina
4eb3b612-8a61-41cc-855e-7ccf917310f7
Kyriazi, Maria Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
El Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Alexaki, Konstantina, Kyriazi, Maria Eleni, El Sagheer, Afaf H., Brown, Tom and Kanaras, Antonios G.
(2020)
Engineering functional nanoparticles for delivery in cells.
Osinski, Marek and Kanaras, Antonios G.
(eds.)
In Colloidal Nanoparticles for Biomedical Applications XV.
vol. 11255,
SPIE..
(doi:10.1117/12.2538470).
Record type:
Conference or Workshop Item
(Paper)
Abstract
The ability of DNA functionalised gold nanoparticles (AuNPs) to detect specific targets in vitro and in vivo has led to their development as suitable tools for sensing applications. However, endosomal entrapment is a common barrier in various nanoparticle delivery approaches. In this work, we present a new design strategy with the aim to enhance endosomal escape of DNA-coated AuNPs via the incorporation of a peptide that has been found to promote effective escape within cells. AuNPs are firstly modified with thiol terminated DNA strands followed by further surface functionalisation with cysteine terminated peptides. We show that optimized loading of peptides following DNA nanoparticle functionalisation of nanoparticles is feasible. DNA-peptide-coated AuNP hybrids show similar stability towards degradation by endocellular enzymes and similar specificity towards the detection of specific mRNA targets.
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e-pub ahead of print date: 21 February 2020
Published date: 2020
Venue - Dates:
Colloidal Nanoparticles for Biomedical Applications XV 2020, , San Francisco, United States, 2020-02-01 - 2020-02-03
Keywords:
DNA, cells, mRNA detection, nanoparticles, peptide
Identifiers
Local EPrints ID: 441771
URI: http://eprints.soton.ac.uk/id/eprint/441771
PURE UUID: 03765ee3-9cd4-48a6-9b23-89911b9b87e6
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Date deposited: 26 Jun 2020 16:33
Last modified: 17 Mar 2024 03:12
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Contributors
Author:
Konstantina Alexaki
Author:
Maria Eleni Kyriazi
Author:
Afaf H. El Sagheer
Editor:
Marek Osinski
Editor:
Antonios G. Kanaras
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