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Engineering functional nanoparticles for delivery in cells

Engineering functional nanoparticles for delivery in cells
Engineering functional nanoparticles for delivery in cells

The ability of DNA functionalised gold nanoparticles (AuNPs) to detect specific targets in vitro and in vivo has led to their development as suitable tools for sensing applications. However, endosomal entrapment is a common barrier in various nanoparticle delivery approaches. In this work, we present a new design strategy with the aim to enhance endosomal escape of DNA-coated AuNPs via the incorporation of a peptide that has been found to promote effective escape within cells. AuNPs are firstly modified with thiol terminated DNA strands followed by further surface functionalisation with cysteine terminated peptides. We show that optimized loading of peptides following DNA nanoparticle functionalisation of nanoparticles is feasible. DNA-peptide-coated AuNP hybrids show similar stability towards degradation by endocellular enzymes and similar specificity towards the detection of specific mRNA targets.

Cells, DNA, Nanoparticles, Peptide, RNA detection
SPIE
Alexaki, Konstantina
4eb3b612-8a61-41cc-855e-7ccf917310f7
Kyriazi, Maria Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
El Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Osinski, Marek
Kanaras, Antonios G.
Alexaki, Konstantina
4eb3b612-8a61-41cc-855e-7ccf917310f7
Kyriazi, Maria Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
El Sagheer, Afaf H.
05b8295a-64ad-4fdf-ad57-c34934a46c04
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Osinski, Marek
Kanaras, Antonios G.

Alexaki, Konstantina, Kyriazi, Maria Eleni, El Sagheer, Afaf H., Brown, Tom and Kanaras, Antonios G. (2020) Engineering functional nanoparticles for delivery in cells. Osinski, Marek and Kanaras, Antonios G. (eds.) In Colloidal Nanoparticles for Biomedical Applications XV. vol. 11255, SPIE.. (doi:10.1117/12.2538470).

Record type: Conference or Workshop Item (Paper)

Abstract

The ability of DNA functionalised gold nanoparticles (AuNPs) to detect specific targets in vitro and in vivo has led to their development as suitable tools for sensing applications. However, endosomal entrapment is a common barrier in various nanoparticle delivery approaches. In this work, we present a new design strategy with the aim to enhance endosomal escape of DNA-coated AuNPs via the incorporation of a peptide that has been found to promote effective escape within cells. AuNPs are firstly modified with thiol terminated DNA strands followed by further surface functionalisation with cysteine terminated peptides. We show that optimized loading of peptides following DNA nanoparticle functionalisation of nanoparticles is feasible. DNA-peptide-coated AuNP hybrids show similar stability towards degradation by endocellular enzymes and similar specificity towards the detection of specific mRNA targets.

Full text not available from this repository.

More information

e-pub ahead of print date: 21 February 2020
Venue - Dates: Colloidal Nanoparticles for Biomedical Applications XV 2020, United States, 2020-02-01 - 2020-02-03
Keywords: Cells, DNA, Nanoparticles, Peptide, RNA detection

Identifiers

Local EPrints ID: 441771
URI: http://eprints.soton.ac.uk/id/eprint/441771
PURE UUID: 03765ee3-9cd4-48a6-9b23-89911b9b87e6
ORCID for Konstantina Alexaki: ORCID iD orcid.org/0000-0002-8702-2696
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 26 Jun 2020 16:33
Last modified: 27 Jun 2020 00:39

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