The University of Southampton
University of Southampton Institutional Repository

Bactericidal effect of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters against multidrug-resistant Neisseria gonorrhoeae

Bactericidal effect of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters against multidrug-resistant Neisseria gonorrhoeae
Bactericidal effect of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters against multidrug-resistant Neisseria gonorrhoeae

Neisseria gonorrhoeae is among the most multidrug-resistant bacteria in circulation today, and new treatments are urgently needed. In this work, we demonstrate the ability of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters (MNBA-AgNCs) to kill strains of Neisseria gonorrhoeae. Using an in vitro bactericidal assay, MNBA-AgNCs had been found to show significantly higher anti-gonococcal bioactivity than the antibiotics ceftriaxone and azithromycin and silver nitrate. These nanoclusters were effective against both planktonic bacteria and a gonococcal infection of human cell cultures in vitro. Treatment of human cells in vitro with MNBA-AgNCs did not induce significant release of lactate dehydrogenase, suggesting minimal cytotoxicity to eukaryotic cells. Our results suggest that MNBA-AgNCs hold great potential for topical treatment of localized gonorrhoeae.

antibacterial properties, azithromycin, ceftriaxone, human cells, nanoparticles, Neisseria gonorrhoeae, silver nanoclusters
1944-8244
27994–28003
Lucío, María Isabel
496d5f14-3826-4ef1-9522-77b6b0cd988c
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Hamilton, Joshua
09930fc3-07c1-464d-8aca-45c106fae3e7
Batista, Diego
0e67849b-da8e-4190-ac51-e6467018afcc
Sheppard, Alexander
bd1cf051-ee46-427f-857b-ef61ee587cdb
Sams-Dodd, Elisabeth
de640659-c6e7-4d83-b534-178315313b2e
Humbert, Maria Victoria
82134d25-24b8-4fdd-bd1c-461683b5322e
Hussain, Irshad
6fcf2f10-9969-4d33-9169-ca89845ef720
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7
Lucío, María Isabel
496d5f14-3826-4ef1-9522-77b6b0cd988c
Kyriazi, Maria-Eleni
3cfe9662-4e7f-49bc-b707-ccc2b4da6b09
Hamilton, Joshua
09930fc3-07c1-464d-8aca-45c106fae3e7
Batista, Diego
0e67849b-da8e-4190-ac51-e6467018afcc
Sheppard, Alexander
bd1cf051-ee46-427f-857b-ef61ee587cdb
Sams-Dodd, Elisabeth
de640659-c6e7-4d83-b534-178315313b2e
Humbert, Maria Victoria
82134d25-24b8-4fdd-bd1c-461683b5322e
Hussain, Irshad
6fcf2f10-9969-4d33-9169-ca89845ef720
Christodoulides, Myron
eba99148-620c-452a-a334-c1a52ba94078
Kanaras, Antonios G.
667ecfdc-7647-4bd8-be03-a47bf32504c7

Lucío, María Isabel, Kyriazi, Maria-Eleni, Hamilton, Joshua, Batista, Diego, Sheppard, Alexander, Sams-Dodd, Elisabeth, Humbert, Maria Victoria, Hussain, Irshad, Christodoulides, Myron and Kanaras, Antonios G. (2020) Bactericidal effect of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters against multidrug-resistant Neisseria gonorrhoeae. ACS Applied Materials and Interfaces, 12 (25), 27994–28003. (doi:10.1021/acsami.0c06163).

Record type: Article

Abstract

Neisseria gonorrhoeae is among the most multidrug-resistant bacteria in circulation today, and new treatments are urgently needed. In this work, we demonstrate the ability of 5-mercapto-2-nitrobenzoic acid-coated silver nanoclusters (MNBA-AgNCs) to kill strains of Neisseria gonorrhoeae. Using an in vitro bactericidal assay, MNBA-AgNCs had been found to show significantly higher anti-gonococcal bioactivity than the antibiotics ceftriaxone and azithromycin and silver nitrate. These nanoclusters were effective against both planktonic bacteria and a gonococcal infection of human cell cultures in vitro. Treatment of human cells in vitro with MNBA-AgNCs did not induce significant release of lactate dehydrogenase, suggesting minimal cytotoxicity to eukaryotic cells. Our results suggest that MNBA-AgNCs hold great potential for topical treatment of localized gonorrhoeae.

Text
bacteria_Kanaras - Accepted Manuscript
Download (707kB)

More information

Accepted/In Press date: 2 June 2020
e-pub ahead of print date: 12 June 2020
Published date: 24 June 2020
Keywords: antibacterial properties, azithromycin, ceftriaxone, human cells, nanoparticles, Neisseria gonorrhoeae, silver nanoclusters

Identifiers

Local EPrints ID: 441799
URI: http://eprints.soton.ac.uk/id/eprint/441799
ISSN: 1944-8244
PURE UUID: fef2e58c-f2d9-447b-b33c-7bca93fcdf49
ORCID for Maria Victoria Humbert: ORCID iD orcid.org/0000-0002-5728-6981
ORCID for Myron Christodoulides: ORCID iD orcid.org/0000-0002-9663-4731
ORCID for Antonios G. Kanaras: ORCID iD orcid.org/0000-0002-9847-6706

Catalogue record

Date deposited: 26 Jun 2020 16:45
Last modified: 18 Mar 2024 05:26

Export record

Altmetrics

Contributors

Author: María Isabel Lucío
Author: Maria-Eleni Kyriazi
Author: Joshua Hamilton
Author: Diego Batista
Author: Alexander Sheppard
Author: Elisabeth Sams-Dodd
Author: Maria Victoria Humbert ORCID iD
Author: Irshad Hussain

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×