Interactions of a bacterial RND transporter with a transmembrane small protein in a lipid environment

Accepted/In Press date: 27 March 2020

e-pub ahead of print date: 28 March 2020

Published date: 2 June 2020

Additional Information: Funding Information: The work was supported by an ERC advanced award ( 742210 ) and by a Wellcome Trust Investigator award to B.F.L. C.E.N. was supported by a Gates-Cambridge Scholarship and A.N. by a Herchel Smith Research Studentship. Work by L.M.R., M.W.O., and G.S. was supported by the Intramural Program of the Eunice Kennedy Shriver National Institute of Child Health and Human Development . We acknowledge Diamond for access and support of the cryo-EM facilities at the UK National Electron Bio-Imaging Centre (eBIC), funded by the Wellcome Trust , MRC , and BBSRC . We thank colleagues at eBIC, especially Dan Clare and Alistair Siebert, for help with cryo-EM data collection. We thank Dima Chirgadze for help with data collection using the University of Cambridge cryo-EM facility. We also thank Douglas B. Weibel for kindly providing strains deficient in cardiolipin biogenesis and Jacob Olondo Kuba for help generating some AcrZ mutants. We are extremely grateful to Tristan Croll for advice and help with ISOLDE for the refinement of the models, and to Anirban Banerjee, Alex Sodt, Yvette Nstogo and Malitha Ratnaweera for helpful and insightful discussions. Publisher Copyright: © 2020 The Authors

Keywords: allostery, antibiotic, cryoEM, drug efflux, molecular dynamics, small protein, structural model, transmembrane transport

Local EPrints ID: 441826

URI: http://eprints.soton.ac.uk/id/eprint/441826

DOI: doi:10.1016/j.str2020.03.013

ISSN: 0969-2126

PURE UUID: e99117df-35ec-430a-9d02-a75de3cfd435

ORCID for Firdaus Samsudin: orcid.org/0000-0003-2766-4459

ORCID for Syma Khalid: orcid.org/0000-0002-3694-5044

Date deposited: 29 Jun 2020 16:33

Last modified: 17 Mar 2024 03:11