3D models of chondrocytes within biomimetic scaffolds: effects of cell deformation from loading regimens
3D models of chondrocytes within biomimetic scaffolds: effects of cell deformation from loading regimens
Background
Mechanical conditioning has been widely used to attempt to enhance chondrocyte metabolism for the evolution of functionally competent cartilage. However, although upregulation of proteoglycans have been reported through the application of uniaxial compression, minimal collagen has been produced. The study is designed to examine whether alternative loading regimens, equivalent to physiological conditions, involving shear in addition to compression can enhance collagen production.
Methods
Finite element models were developed to determine how the local chondrocyte environments within agarose constructs were influenced by a range of static and dynamic loading regimens. 3-D poro-viscoelastic models were validated against experimental data. In particular, these models were used to characterise chondrocyte deformation in compression with and without shear superimposed, with special reference to the formation of pericellular matrix around the cells.
Findings
The models of the hydrogel constructs under stress relaxation and dynamic cyclic compression conditions were highly correlated with the experimental data. The cell deformation (y/z) in the constructs was greatest in the centre of the constructs, increasing with magnitude of compression up to 25%. The superposition of shear however did not produce significant additional changes in deformation, with the presence of PCM reducing the chondrocyte deformation.
Interpretation
The use of FE models can prove important in the definition of appropriate, optimised mechanical conditioning regimens for the synthesis and organisation of mature extra cellular matrix by chondrocyte-seeded constructs. They will also provide insight into the mechanisms relating cell deformation to mechanotransduction pathways, thereby progressing the development of functionally competent tissue engineered cartilage.
Cartilage tissue engineering, Cell deformation, Finite element analysis, Scaffold
1-10
Di Federico, Erica
66063cda-3d6c-4465-bedc-c63ea3d2ff56
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Shelton, Julia C.
7f4338a2-14d3-429a-a051-eb17bf38b879
October 2020
Di Federico, Erica
66063cda-3d6c-4465-bedc-c63ea3d2ff56
Bader, Dan L.
9884d4f6-2607-4d48-bf0c-62bdcc0d1dbf
Shelton, Julia C.
7f4338a2-14d3-429a-a051-eb17bf38b879
Di Federico, Erica, Bader, Dan L. and Shelton, Julia C.
(2020)
3D models of chondrocytes within biomimetic scaffolds: effects of cell deformation from loading regimens.
Clinical Biomechanics, 79, , [104972].
(doi:10.1016/j.clinbiomech.2020.01.022).
Abstract
Background
Mechanical conditioning has been widely used to attempt to enhance chondrocyte metabolism for the evolution of functionally competent cartilage. However, although upregulation of proteoglycans have been reported through the application of uniaxial compression, minimal collagen has been produced. The study is designed to examine whether alternative loading regimens, equivalent to physiological conditions, involving shear in addition to compression can enhance collagen production.
Methods
Finite element models were developed to determine how the local chondrocyte environments within agarose constructs were influenced by a range of static and dynamic loading regimens. 3-D poro-viscoelastic models were validated against experimental data. In particular, these models were used to characterise chondrocyte deformation in compression with and without shear superimposed, with special reference to the formation of pericellular matrix around the cells.
Findings
The models of the hydrogel constructs under stress relaxation and dynamic cyclic compression conditions were highly correlated with the experimental data. The cell deformation (y/z) in the constructs was greatest in the centre of the constructs, increasing with magnitude of compression up to 25%. The superposition of shear however did not produce significant additional changes in deformation, with the presence of PCM reducing the chondrocyte deformation.
Interpretation
The use of FE models can prove important in the definition of appropriate, optimised mechanical conditioning regimens for the synthesis and organisation of mature extra cellular matrix by chondrocyte-seeded constructs. They will also provide insight into the mechanisms relating cell deformation to mechanotransduction pathways, thereby progressing the development of functionally competent tissue engineered cartilage.
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More information
Accepted/In Press date: 28 January 2020
e-pub ahead of print date: 8 February 2020
Published date: October 2020
Additional Information:
Copyright © 2020. Published by Elsevier Ltd.
Keywords:
Cartilage tissue engineering, Cell deformation, Finite element analysis, Scaffold
Identifiers
Local EPrints ID: 441835
URI: http://eprints.soton.ac.uk/id/eprint/441835
ISSN: 0268-0033
PURE UUID: d221021d-0877-4482-a97b-2068b5fd75a8
Catalogue record
Date deposited: 29 Jun 2020 16:36
Last modified: 17 Mar 2024 05:26
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Contributors
Author:
Erica Di Federico
Author:
Julia C. Shelton
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