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Hierarchical imaging of the human knee

Hierarchical imaging of the human knee
Hierarchical imaging of the human knee

Among the clinically relevant imaging techniques, computed tomography (CT) reaches the best spatial resolution. Sub-millimeter voxel sizes are regularly obtained. For investigations on true micrometer level lab-based μCT has become gold standard. The aim of the present study is the hierarchical investigation of a human knee post mortem using hard X-ray μCT. After the visualization of the entire knee using a clinical CT with a spatial resolution on the sub-millimeter range, a hierarchical imaging study was performed using a laboratory μCT system nanotom® m. Due to the size of the whole knee the pixel length could not be reduced below 65 μm. These first two data sets were directly compared after a rigid registration using a cross-correlation algorithm. The μCT data set allowed an investigation of the trabecular structures of the bones. The further reduction of the pixel length down to 25 μm could be achieved by removing the skin and soft tissues and measuring the tibia and the femur separately. True micrometer resolution could be achieved after extracting cylinders of several millimeters diameters from the two bones. The high resolution scans revealed the mineralized cartilage zone including the tide mark line as well as individual calcified chondrocytes. The visualization of soft tissues including cartilage, was arranged by X-ray grating interferometry (XGI) at ESRF and Diamond Light Source. Whereas the high-energy measurements at ESRF allowed the simultaneous visualization of soft and hard tissues, the low-energy results from Diamond Light Source made individual chondrocytes within the cartilage visual.

cartilage, grating interferometry, human knee, laboratory X-ray source, Microtomography, X-ray phase contrast
SPIE
Schulz, Georg
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Götz, Christian
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Deyhle, Hans
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Müller-Gerbl, Magdalena
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Zanette, Irene
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Zdora, Marie Christine
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Khimchenko, Anna
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Thalmann, Peter
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Rack, Alexander
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Müller, Bert
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Wang, Ge
Stock, Stuart R.
Muller, Bert
Schulz, Georg
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Götz, Christian
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Deyhle, Hans
aba9cd34-97a0-4238-8255-af673e3beb1a
Müller-Gerbl, Magdalena
366449c8-781b-4713-abaa-7abfd3ecd489
Zanette, Irene
39ee899c-0aae-4fac-aec6-826f848a8022
Zdora, Marie Christine
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Khimchenko, Anna
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Thalmann, Peter
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Rack, Alexander
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Müller, Bert
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Wang, Ge
Stock, Stuart R.
Muller, Bert

Schulz, Georg, Götz, Christian, Deyhle, Hans, Müller-Gerbl, Magdalena, Zanette, Irene, Zdora, Marie Christine, Khimchenko, Anna, Thalmann, Peter, Rack, Alexander and Müller, Bert (2016) Hierarchical imaging of the human knee. Wang, Ge, Stock, Stuart R. and Muller, Bert (eds.) In Developments in X-Ray Tomography X. vol. 9967, SPIE.. (doi:10.1117/12.2238089).

Record type: Conference or Workshop Item (Paper)

Abstract

Among the clinically relevant imaging techniques, computed tomography (CT) reaches the best spatial resolution. Sub-millimeter voxel sizes are regularly obtained. For investigations on true micrometer level lab-based μCT has become gold standard. The aim of the present study is the hierarchical investigation of a human knee post mortem using hard X-ray μCT. After the visualization of the entire knee using a clinical CT with a spatial resolution on the sub-millimeter range, a hierarchical imaging study was performed using a laboratory μCT system nanotom® m. Due to the size of the whole knee the pixel length could not be reduced below 65 μm. These first two data sets were directly compared after a rigid registration using a cross-correlation algorithm. The μCT data set allowed an investigation of the trabecular structures of the bones. The further reduction of the pixel length down to 25 μm could be achieved by removing the skin and soft tissues and measuring the tibia and the femur separately. True micrometer resolution could be achieved after extracting cylinders of several millimeters diameters from the two bones. The high resolution scans revealed the mineralized cartilage zone including the tide mark line as well as individual calcified chondrocytes. The visualization of soft tissues including cartilage, was arranged by X-ray grating interferometry (XGI) at ESRF and Diamond Light Source. Whereas the high-energy measurements at ESRF allowed the simultaneous visualization of soft and hard tissues, the low-energy results from Diamond Light Source made individual chondrocytes within the cartilage visual.

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More information

Published date: 4 October 2016
Venue - Dates: Developments in X-Ray Tomography X, United States, 2016-08-28 - 2016-08-30
Keywords: cartilage, grating interferometry, human knee, laboratory X-ray source, Microtomography, X-ray phase contrast

Identifiers

Local EPrints ID: 441901
URI: http://eprints.soton.ac.uk/id/eprint/441901
PURE UUID: 2bae95b8-2175-4be5-bdd3-fce77c189e55

Catalogue record

Date deposited: 01 Jul 2020 16:35
Last modified: 07 Oct 2020 00:39

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Contributors

Author: Georg Schulz
Author: Christian Götz
Author: Hans Deyhle
Author: Magdalena Müller-Gerbl
Author: Irene Zanette
Author: Marie Christine Zdora
Author: Anna Khimchenko
Author: Peter Thalmann
Author: Alexander Rack
Author: Bert Müller
Editor: Ge Wang
Editor: Stuart R. Stock
Editor: Bert Muller

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