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Involvement of nitric oxide in biofilm dispersal of Pseudomonas aeruginosa

Involvement of nitric oxide in biofilm dispersal of Pseudomonas aeruginosa
Involvement of nitric oxide in biofilm dispersal of Pseudomonas aeruginosa
Bacterial biofilms at times undergo regulated and coordinated dispersal events where sessile biofilm cells convert to free-swimming, planktonic bacteria. In the opportunistic pathogen Pseudomonas aeruginosa, we previously observed that dispersal occurs concurrently with three interrelated processes within mature biofilms: (i) production of oxidative or nitrosative stress-inducing molecules inside biofilm structures, (ii) bacteriophage induction, and (iii) cell lysis. Here we examine whether specific reactive oxygen or nitrogen intermediates play a role in cell dispersal from P. aeruginosa biofilms. We demonstrate the involvement of anaerobic respiration processes in P. aeruginosa biofilm dispersal and show that nitric oxide (NO), used widely as a signaling molecule in biological systems, causes dispersal of P. aeruginosa biofilm bacteria. Dispersal was induced with low, sublethal concentrations (25 to 500 nM) of the NO donor sodium nitroprusside (SNP). Moreover, a P. aeruginosa mutant lacking the only enzyme capable of generating metabolic NO through anaerobic respiration (nitrite reductase, nirS) did not disperse, whereas a NO reductase mutant (norCB) exhibited greatly enhanced dispersal. Strategies to induce biofilm dispersal are of interest due to their potential to prevent biofilms and biofilm-related infections. We observed that exposure to SNP (500 nM) greatly enhanced the efficacy of antimicrobial compounds (tobramycin, hydrogen peroxide, and sodium dodecyl sulfate) in the removal of established P. aeruginosa biofilms from a glass surface. Combined exposure to both NO and antimicrobial agents may therefore offer a novel strategy to control preestablished, persistent P. aeruginosa biofilms and biofilm-related infections.
Biofilms, Microbiology, nitric oxide, dispersal
0021-9193
7344-7353
Barraud, Nicolas
01e480a6-d225-44eb-acde-433c5b24bb82
Hassett, Daniel J.
5d8fe550-d498-4b3d-beba-f11519251681
Hwang, Sung-Hei
c18d0d40-b970-4302-bdb6-0a5bb5f1688e
Rice, Scott A.
4f9516db-1d35-4211-878c-bb6cfb2a6b4a
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Barraud, Nicolas
01e480a6-d225-44eb-acde-433c5b24bb82
Hassett, Daniel J.
5d8fe550-d498-4b3d-beba-f11519251681
Hwang, Sung-Hei
c18d0d40-b970-4302-bdb6-0a5bb5f1688e
Rice, Scott A.
4f9516db-1d35-4211-878c-bb6cfb2a6b4a
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d

Barraud, Nicolas, Hassett, Daniel J., Hwang, Sung-Hei, Rice, Scott A., Kjelleberg, Staffan and Webb, Jeremy S. (2006) Involvement of nitric oxide in biofilm dispersal of Pseudomonas aeruginosa. Journal of bacteriology, 188 (21), 7344-7353. (doi:10.1128/JB.00779-06).

Record type: Article

Abstract

Bacterial biofilms at times undergo regulated and coordinated dispersal events where sessile biofilm cells convert to free-swimming, planktonic bacteria. In the opportunistic pathogen Pseudomonas aeruginosa, we previously observed that dispersal occurs concurrently with three interrelated processes within mature biofilms: (i) production of oxidative or nitrosative stress-inducing molecules inside biofilm structures, (ii) bacteriophage induction, and (iii) cell lysis. Here we examine whether specific reactive oxygen or nitrogen intermediates play a role in cell dispersal from P. aeruginosa biofilms. We demonstrate the involvement of anaerobic respiration processes in P. aeruginosa biofilm dispersal and show that nitric oxide (NO), used widely as a signaling molecule in biological systems, causes dispersal of P. aeruginosa biofilm bacteria. Dispersal was induced with low, sublethal concentrations (25 to 500 nM) of the NO donor sodium nitroprusside (SNP). Moreover, a P. aeruginosa mutant lacking the only enzyme capable of generating metabolic NO through anaerobic respiration (nitrite reductase, nirS) did not disperse, whereas a NO reductase mutant (norCB) exhibited greatly enhanced dispersal. Strategies to induce biofilm dispersal are of interest due to their potential to prevent biofilms and biofilm-related infections. We observed that exposure to SNP (500 nM) greatly enhanced the efficacy of antimicrobial compounds (tobramycin, hydrogen peroxide, and sodium dodecyl sulfate) in the removal of established P. aeruginosa biofilms from a glass surface. Combined exposure to both NO and antimicrobial agents may therefore offer a novel strategy to control preestablished, persistent P. aeruginosa biofilms and biofilm-related infections.

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Submitted date: 31 May 2006
Published date: 1 November 2006
Keywords: Biofilms, Microbiology, nitric oxide, dispersal

Identifiers

Local EPrints ID: 44192
URI: https://eprints.soton.ac.uk/id/eprint/44192
ISSN: 0021-9193
PURE UUID: 8a01a90b-b318-4eed-afc1-1a7e1b52a65f
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

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Date deposited: 19 Feb 2007
Last modified: 14 Mar 2019 01:41

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Contributors

Author: Nicolas Barraud
Author: Daniel J. Hassett
Author: Sung-Hei Hwang
Author: Scott A. Rice
Author: Staffan Kjelleberg
Author: Jeremy S. Webb ORCID iD

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