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Involvement of nitric oxide in biofilm dipsersal of Pseudomonas aeruginosa

Record type: Article

Bacterial biofilms at times undergo regulated and coordinated dispersal events where sessile biofilm cells convert to free-swimming, planktonic bacteria. In the opportunistic pathogen Pseudomonas aeruginosa, we previously observed that dispersal occurs concurrently with three interrelated processes within mature biofilms: (i) production of oxidative or nitrosative stress-inducing molecules inside biofilm structures, (ii) bacteriophage induction, and (iii) cell lysis. Here we examine whether specific reactive oxygen or nitrogen intermediates play a role in cell dispersal from P. aeruginosa biofilms. We demonstrate the involvement of anaerobic respiration processes in P. aeruginosa biofilm dispersal and show that nitric oxide (NO), used widely as a signaling molecule in biological systems, causes dispersal of P. aeruginosa biofilm bacteria. Dispersal was induced with low, sublethal concentrations (25 to 500 nM) of the NO donor sodium nitroprusside (SNP). Moreover, a P. aeruginosa mutant lacking the only enzyme capable of generating metabolic NO through anaerobic respiration (nitrite reductase, nirS) did not disperse, whereas a NO reductase mutant (norCB) exhibited greatly enhanced dispersal. Strategies to induce biofilm dispersal are of interest due to their potential to prevent biofilms and biofilm-related infections. We observed that exposure to SNP (500 nM) greatly enhanced the efficacy of antimicrobial compounds (tobramycin, hydrogen peroxide, and sodium dodecyl sulfate) in the removal of established P. aeruginosa biofilms from a glass surface. Combined exposure to both NO and antimicrobial agents may therefore offer a novel strategy to control preestablished, persistent P. aeruginosa biofilms and biofilm-related infections.

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Citation

Barraud, Nicolas, Hassett, Daniel J., Hwang, Sung-Hei, Rice, Scott A., Kjelleberg, Staffan and Webb, Jeremy S. (2006) Involvement of nitric oxide in biofilm dipsersal of Pseudomonas aeruginosa Journal of Bacteriology, 188, (21), pp. 7344-7353. (doi:10.1128/JB.00779-06).

More information

Published date: 2006
Keywords: Biofilms, Microbiology, nitric oxide, dispersal

Identifiers

Local EPrints ID: 44192
URI: http://eprints.soton.ac.uk/id/eprint/44192
ISSN: 0021-9193
PURE UUID: 8a01a90b-b318-4eed-afc1-1a7e1b52a65f
ORCID for Jeremy S. Webb: ORCID iD orcid.org/0000-0003-2068-8589

Catalogue record

Date deposited: 19 Feb 2007
Last modified: 17 Jul 2017 15:15

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Contributors

Author: Nicolas Barraud
Author: Daniel J. Hassett
Author: Sung-Hei Hwang
Author: Scott A. Rice
Author: Staffan Kjelleberg
Author: Jeremy S. Webb ORCID iD

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