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X-ray micro-tomography for investigations of brain tissues on cellular level

X-ray micro-tomography for investigations of brain tissues on cellular level
X-ray micro-tomography for investigations of brain tissues on cellular level

X-ray imaging in absorption contrast mode is well established for hard tissue visualization. However, performance for lower density materials is limited due to a reduced contrast. Our aim is three-dimensional (3D) characterization of micro-morphology of human brain tissues down to (sub-)cellular resolution within a laboratory environment. Using the laboratory-based microtomography (μCT) system nanotom® m (GE Sensing & Inspection Technologies GmbH, Wunstorf, Germany) and synchrotron radiation at the Diamond-Manchester Imaging Branchline I13-2 (Diamond Light Source, Didcot, UK), we have acquired 3D data with a resolution down to 0.45 μm for visualization of a human cerebellum specimen down to cellular level. We have shown that all selected modalities, namely laboratory-based absorption contrast micro-tomography (LBμCT), synchrotron radiation based in-line single distance phase contrast tomography (SDPR) and synchrotron radiation based single-grating interferometry (GI), can reach cellular resolution for tissue samples with a size in the mm-range. The results are discussed qualitatively in comparison to optical microscopy of haematoxylin and eosin (H&E) stained sections. As phase contrast yields to a better data quality for soft tissues and in order to overcome restrictions of limited beamline access for phase contrast measurements, we have equipped the μCT system nanotom® m with a double-grating phase contrast set-up. Preliminary experimental results of a knee sample consisting of a bony part and a cartilage demonstrate that phase contrast data exhibits better quality compared to absorption contrast. Currently, the set-up is under adjustment. It is expected that cellular resolution would also be achieved. The questions arise (1) what would be the quality gain of laboratory-based phase contrast in comparison to laboratory-based absorption contrast tomography and (2) could laboratory-based phase contrast data provide comparable results to synchrotron radiation based phase contrast data.

absorption contrast, brain tissue, cerebellum, grating interferometry, phase contrast, synchrotron radiation, X-ray micro-tomography
SPIE
Khimchenko, Anna
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Schulz, Georg
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Deyhle, Hans
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Thalmann, Peter
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Zanette, Irene
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Zdora, Marie Christine
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Bikis, Christos
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Hipp, Alexander
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Hieber, Simone E.
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Schweighauser, Gabriel
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Hench, Jürgen
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Müller, Bert
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Wang, Ge
Stock, Stuart R.
Muller, Bert
Khimchenko, Anna
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Schulz, Georg
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Deyhle, Hans
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Thalmann, Peter
1eeb6c4a-6982-4c0a-88cc-b4919b4600b9
Zanette, Irene
39ee899c-0aae-4fac-aec6-826f848a8022
Zdora, Marie Christine
a2e3b04b-aef4-42f8-9e96-4707149589fb
Bikis, Christos
af1dad90-61ce-4db8-9423-afbda7f0ab4f
Hipp, Alexander
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Hieber, Simone E.
fb023e2a-5482-4ad9-bc39-ed3608a3225b
Schweighauser, Gabriel
464beb88-86c5-4802-ad27-58b9ca1f3dd8
Hench, Jürgen
8e7e7437-e686-4be1-a615-2090dc73e57d
Müller, Bert
acba4294-b684-4a09-81ac-32de31d39923
Wang, Ge
Stock, Stuart R.
Muller, Bert

Khimchenko, Anna, Schulz, Georg, Deyhle, Hans, Thalmann, Peter, Zanette, Irene, Zdora, Marie Christine, Bikis, Christos, Hipp, Alexander, Hieber, Simone E., Schweighauser, Gabriel, Hench, Jürgen and Müller, Bert (2016) X-ray micro-tomography for investigations of brain tissues on cellular level. Wang, Ge, Stock, Stuart R. and Muller, Bert (eds.) In Developments in X-ray tomography X. vol. 9967, SPIE. 11 pp . (doi:10.1117/12.2237554).

Record type: Conference or Workshop Item (Paper)

Abstract

X-ray imaging in absorption contrast mode is well established for hard tissue visualization. However, performance for lower density materials is limited due to a reduced contrast. Our aim is three-dimensional (3D) characterization of micro-morphology of human brain tissues down to (sub-)cellular resolution within a laboratory environment. Using the laboratory-based microtomography (μCT) system nanotom® m (GE Sensing & Inspection Technologies GmbH, Wunstorf, Germany) and synchrotron radiation at the Diamond-Manchester Imaging Branchline I13-2 (Diamond Light Source, Didcot, UK), we have acquired 3D data with a resolution down to 0.45 μm for visualization of a human cerebellum specimen down to cellular level. We have shown that all selected modalities, namely laboratory-based absorption contrast micro-tomography (LBμCT), synchrotron radiation based in-line single distance phase contrast tomography (SDPR) and synchrotron radiation based single-grating interferometry (GI), can reach cellular resolution for tissue samples with a size in the mm-range. The results are discussed qualitatively in comparison to optical microscopy of haematoxylin and eosin (H&E) stained sections. As phase contrast yields to a better data quality for soft tissues and in order to overcome restrictions of limited beamline access for phase contrast measurements, we have equipped the μCT system nanotom® m with a double-grating phase contrast set-up. Preliminary experimental results of a knee sample consisting of a bony part and a cartilage demonstrate that phase contrast data exhibits better quality compared to absorption contrast. Currently, the set-up is under adjustment. It is expected that cellular resolution would also be achieved. The questions arise (1) what would be the quality gain of laboratory-based phase contrast in comparison to laboratory-based absorption contrast tomography and (2) could laboratory-based phase contrast data provide comparable results to synchrotron radiation based phase contrast data.

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More information

Published date: 1 January 2016
Venue - Dates: SPIE Optical Engineering + Applications, United States, 2016-08-29 - 2016-08-31
Keywords: absorption contrast, brain tissue, cerebellum, grating interferometry, phase contrast, synchrotron radiation, X-ray micro-tomography

Identifiers

Local EPrints ID: 441982
URI: http://eprints.soton.ac.uk/id/eprint/441982
PURE UUID: d728d9eb-32b2-4d99-ac03-7d51d21edeb5

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Date deposited: 03 Jul 2020 16:31
Last modified: 03 Jul 2020 16:31

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Contributors

Author: Anna Khimchenko
Author: Georg Schulz
Author: Hans Deyhle
Author: Peter Thalmann
Author: Irene Zanette
Author: Marie Christine Zdora
Author: Christos Bikis
Author: Alexander Hipp
Author: Simone E. Hieber
Author: Gabriel Schweighauser
Author: Jürgen Hench
Author: Bert Müller
Editor: Ge Wang
Editor: Stuart R. Stock
Editor: Bert Muller

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