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Quantitative evaluation of hard x-ray damage to biological samples using EUV ptychography

Quantitative evaluation of hard x-ray damage to biological samples using EUV ptychography
Quantitative evaluation of hard x-ray damage to biological samples using EUV ptychography
Coherent diffractive imaging (CDI) has become a standard method on a variety of synchrotron beam lines. The high brilliance short wavelength radiation from these sources can be used to reconstruct attenuation and relative phase of a sample with nanometre resolution via CDI methods. However, the interaction between the sample and high energy ionising radiation can cause degradation to sample structure. We demonstrate, using a laboratory based high harmonic generation (HHG) based extreme ultraviolet (EUV) source, imaging a sample of hippocampal neurons using the ptychography method. The significant increase in contrast of the sample in the EUV light allows identification of damage induced from exposure to 7.3 keV photons, without causing any damage to the sample itself.
Report of talk given at 13th International X-Ray Microscopy Conference (XRM), Diamond Light Source, Oxford, UK, 15-19 Aug 2017
1-4
Baksh, Peter
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Odstrčil, Michal
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Parsons, Aaron
4233ec59-883d-433b-ac17-6fd7a3fafa8b
Bailey, Jo
7cfb3e45-647a-423e-ad00-737cf1d31a65
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Chad, John E.
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Brocklesby, William S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Baksh, Peter
578fea83-9a1c-4dd0-b3f6-66f5552f2344
Odstrčil, Michal
b297d3ec-ed42-4709-9f90-7af79d0644c7
Parsons, Aaron
4233ec59-883d-433b-ac17-6fd7a3fafa8b
Bailey, Jo
7cfb3e45-647a-423e-ad00-737cf1d31a65
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Chad, John E.
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Brocklesby, William S.
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f

Baksh, Peter, Odstrčil, Michal, Parsons, Aaron, Bailey, Jo, Deinhardt, Katrin, Chad, John E., Brocklesby, William S. and Frey, Jeremy G. (2017) Quantitative evaluation of hard x-ray damage to biological samples using EUV ptychography. Journal of Physics Conference Series, 849 (1), 1-4, [12034]. (doi:10.1088/1742-6596/849/1/012034).

Record type: Article

Abstract

Coherent diffractive imaging (CDI) has become a standard method on a variety of synchrotron beam lines. The high brilliance short wavelength radiation from these sources can be used to reconstruct attenuation and relative phase of a sample with nanometre resolution via CDI methods. However, the interaction between the sample and high energy ionising radiation can cause degradation to sample structure. We demonstrate, using a laboratory based high harmonic generation (HHG) based extreme ultraviolet (EUV) source, imaging a sample of hippocampal neurons using the ptychography method. The significant increase in contrast of the sample in the EUV light allows identification of damage induced from exposure to 7.3 keV photons, without causing any damage to the sample itself.
Report of talk given at 13th International X-Ray Microscopy Conference (XRM), Diamond Light Source, Oxford, UK, 15-19 Aug 2017

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Published date: 2017

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Local EPrints ID: 442109
URI: http://eprints.soton.ac.uk/id/eprint/442109
PURE UUID: dadf00d6-c957-49b8-9fb9-a698ac0d3fd2
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298
ORCID for John E. Chad: ORCID iD orcid.org/0000-0001-6442-4281
ORCID for William S. Brocklesby: ORCID iD orcid.org/0000-0002-2123-6712
ORCID for Jeremy G. Frey: ORCID iD orcid.org/0000-0003-0842-4302

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Date deposited: 07 Jul 2020 16:49
Last modified: 18 Feb 2021 17:20

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Contributors

Author: Peter Baksh
Author: Michal Odstrčil
Author: Aaron Parsons
Author: Jo Bailey
Author: John E. Chad ORCID iD
Author: Jeremy G. Frey ORCID iD

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