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Unique genetic and histological signatures of mouse pericardial adipose tissue

Unique genetic and histological signatures of mouse pericardial adipose tissue
Unique genetic and histological signatures of mouse pericardial adipose tissue
Obesity is a major risk factor for a plethora of metabolic disturbances including diabetes and cardiovascular disease. Accumulating evidence is showing that there is an adipose tissue depot-dependent relationship with obesity-induced metabolic dysfunction. While some adipose depots, such as subcutaneous fat, are generally metabolically innocuous, others such as visceral fat, are directly deleterious. A lesser known visceral adipose depot is the pericardial adipose tissue depot. We therefore set out to examine its transcriptional and morphological signature under chow and high-fat fed conditions, in comparison with other adipose depots, using a mouse model. Our results revealed that under chow conditions pericardial adipose tissue has uncoupling-protein 1 gene
expression levels which are significantly higher than classical subcutaneous and visceral adipose depots. We also observed that under high-fat diet conditions, the pericardial adipose depot exhibits greatly upregulated transcript levels of inflammatory cytokines. Our results collectively indicate, for the first time, that the pericardial adipose tissue possesses a unique transcriptional and histological
signature which has features of both a beige (brown fat-like) but also pro-inflammatory depot, such as visceral fat. This unique profile may be involved in metabolic dysfunction associated with obesity.
Adipose tissue, Metabolism, Obesity
2072-6643
1-17
Al-Dibouni, A.
1c209a2d-172b-4884-b387-67379412fee2
Gaspar, R.
276e3de0-4461-4b1b-aa64-7ef5e84c899c
Ige, S.
db7de942-5906-4be1-8dad-8ba281da7f5f
Boateng, S.
4f54a32b-c883-4656-83bf-6490bf0fa60e
Cagampang, F.
7cf57d52-4a65-4554-8306-ed65226bc50e
Gibbins, J.
163ce936-dd83-4ba9-8a6d-dfd5a90c4fd4
Cox, R.
934cb2e6-1142-42ab-b35d-fd0dc6b879a3
Sellayah, D.
236eeb2c-c341-4b5c-934d-5fba6dc60c85
Al-Dibouni, A.
1c209a2d-172b-4884-b387-67379412fee2
Gaspar, R.
276e3de0-4461-4b1b-aa64-7ef5e84c899c
Ige, S.
db7de942-5906-4be1-8dad-8ba281da7f5f
Boateng, S.
4f54a32b-c883-4656-83bf-6490bf0fa60e
Cagampang, F.
7cf57d52-4a65-4554-8306-ed65226bc50e
Gibbins, J.
163ce936-dd83-4ba9-8a6d-dfd5a90c4fd4
Cox, R.
934cb2e6-1142-42ab-b35d-fd0dc6b879a3
Sellayah, D.
236eeb2c-c341-4b5c-934d-5fba6dc60c85

Al-Dibouni, A., Gaspar, R., Ige, S., Boateng, S., Cagampang, F., Gibbins, J., Cox, R. and Sellayah, D. (2020) Unique genetic and histological signatures of mouse pericardial adipose tissue. Nutrients, 12 (6), 1-17, [1855]. (doi:10.3390/nu12061855).

Record type: Article

Abstract

Obesity is a major risk factor for a plethora of metabolic disturbances including diabetes and cardiovascular disease. Accumulating evidence is showing that there is an adipose tissue depot-dependent relationship with obesity-induced metabolic dysfunction. While some adipose depots, such as subcutaneous fat, are generally metabolically innocuous, others such as visceral fat, are directly deleterious. A lesser known visceral adipose depot is the pericardial adipose tissue depot. We therefore set out to examine its transcriptional and morphological signature under chow and high-fat fed conditions, in comparison with other adipose depots, using a mouse model. Our results revealed that under chow conditions pericardial adipose tissue has uncoupling-protein 1 gene
expression levels which are significantly higher than classical subcutaneous and visceral adipose depots. We also observed that under high-fat diet conditions, the pericardial adipose depot exhibits greatly upregulated transcript levels of inflammatory cytokines. Our results collectively indicate, for the first time, that the pericardial adipose tissue possesses a unique transcriptional and histological
signature which has features of both a beige (brown fat-like) but also pro-inflammatory depot, such as visceral fat. This unique profile may be involved in metabolic dysfunction associated with obesity.

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Accepted/In Press date: 19 June 2016
Published date: 22 June 2020
Keywords: Adipose tissue, Metabolism, Obesity

Identifiers

Local EPrints ID: 442110
URI: http://eprints.soton.ac.uk/id/eprint/442110
ISSN: 2072-6643
PURE UUID: 378bcc4b-3028-4384-bbe7-6f0f04c0f463
ORCID for F. Cagampang: ORCID iD orcid.org/0000-0003-4404-9853

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Date deposited: 07 Jul 2020 16:49
Last modified: 23 Jul 2022 01:50

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Contributors

Author: A. Al-Dibouni
Author: R. Gaspar
Author: S. Ige
Author: S. Boateng
Author: F. Cagampang ORCID iD
Author: J. Gibbins
Author: R. Cox
Author: D. Sellayah

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