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Dose-related effects of eicosapentaenoic acid on innate immune function in healthy humans: a comparison of young and older men

Dose-related effects of eicosapentaenoic acid on innate immune function in healthy humans: a comparison of young and older men
Dose-related effects of eicosapentaenoic acid on innate immune function in healthy humans: a comparison of young and older men
BACKGROUND: Increasing intakes of long-chain n-3 polyunsaturated fatty acids (PUFAs) can decrease markers of immunity. However, dose- and age-related responses have not been identified.
OBJECTIVE: The objective was to determine the effects of different amounts of eicosapentaenoic acid (EPA) on innate immune outcomes in young and older males.
DESIGN: In a controlled, double-blind study, healthy young and older men consumed 1 of 4 supplements provided as capsules: placebo (corn oil) or different amounts of an oil providing 1.35, 2.7, or 4.05 g EPA/d for 12 wk. Blood samples were collected at baseline and after 12 wk.
RESULTS: EPA was incorporated in a linear dose-response fashion into plasma and mononuclear cell (MNC) phospholipids; incorporation was greater in the older men. EPA treatment did not alter neutrophil or monocyte phagocytosis, monocyte respiratory burst, or the production of inflammatory cytokines by MNCs in the young or older men. EPA treatment caused a dose-dependent decrease in neutrophil respiratory burst only in the older men. Increased incorporation of EPA into plasma or MNC phospholipids was associated with decreased production of prostaglandin E2 by MNCs from both young and older men.
CONCLUSIONS: Older subjects incorporate EPA into plasma and MNC phospholipids more readily than do younger subjects. Other than prostaglandin E2 production, innate immune responses in young subjects are not affected by an EPA intake of < or =4.05 g/d. Older subjects are more sensitive to the immunologic effects of EPA, and the neutrophil respiratory burst is lower at higher EPA intakes
acid, acids, administration & dosage, adolescent, adult, aged, aging, analysis of variance, biosynthesis, blood, chemistry, comparative study, cytokines, design, dietary supplements, dinoprostone, dose-response relationship, drug, double-blind method, drug effects, eicosapentaenoic acid, fatty acid, fatty acids, human, humans, immunity, natural, immunology, leukocytes, mononuclear, male, metabolism, middle aged, neutrophils, phagocytosis, pharmacology, phospholipids, physiology, plasma, respiratory burst
0002-9165
331-342
Rees, Dinka
08dffec4-2d93-48da-b451-ed57fb048e33
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Banerjee, Tapati
268e92f6-7802-44b4-a916-746c2433fa71
Wells, Solenne J.
9e44d0d4-3138-48fc-baaf-c1f00f37f1d0
Roynette, Catherine E.
08826cd4-6fab-4904-9123-59cdeca5cdc4
Wahle, Klaus W.J.
88716de8-fff4-48bd-9434-cbb6c15ba02e
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6
Rees, Dinka
08dffec4-2d93-48da-b451-ed57fb048e33
Miles, Elizabeth A.
20332899-ecdb-4214-95bc-922dde36d416
Banerjee, Tapati
268e92f6-7802-44b4-a916-746c2433fa71
Wells, Solenne J.
9e44d0d4-3138-48fc-baaf-c1f00f37f1d0
Roynette, Catherine E.
08826cd4-6fab-4904-9123-59cdeca5cdc4
Wahle, Klaus W.J.
88716de8-fff4-48bd-9434-cbb6c15ba02e
Calder, Philip C.
1797e54f-378e-4dcb-80a4-3e30018f07a6

Rees, Dinka, Miles, Elizabeth A., Banerjee, Tapati, Wells, Solenne J., Roynette, Catherine E., Wahle, Klaus W.J. and Calder, Philip C. (2006) Dose-related effects of eicosapentaenoic acid on innate immune function in healthy humans: a comparison of young and older men. American Journal of Clinical Nutrition, 83 (2), 331-342.

Record type: Article

Abstract

BACKGROUND: Increasing intakes of long-chain n-3 polyunsaturated fatty acids (PUFAs) can decrease markers of immunity. However, dose- and age-related responses have not been identified.
OBJECTIVE: The objective was to determine the effects of different amounts of eicosapentaenoic acid (EPA) on innate immune outcomes in young and older males.
DESIGN: In a controlled, double-blind study, healthy young and older men consumed 1 of 4 supplements provided as capsules: placebo (corn oil) or different amounts of an oil providing 1.35, 2.7, or 4.05 g EPA/d for 12 wk. Blood samples were collected at baseline and after 12 wk.
RESULTS: EPA was incorporated in a linear dose-response fashion into plasma and mononuclear cell (MNC) phospholipids; incorporation was greater in the older men. EPA treatment did not alter neutrophil or monocyte phagocytosis, monocyte respiratory burst, or the production of inflammatory cytokines by MNCs in the young or older men. EPA treatment caused a dose-dependent decrease in neutrophil respiratory burst only in the older men. Increased incorporation of EPA into plasma or MNC phospholipids was associated with decreased production of prostaglandin E2 by MNCs from both young and older men.
CONCLUSIONS: Older subjects incorporate EPA into plasma and MNC phospholipids more readily than do younger subjects. Other than prostaglandin E2 production, innate immune responses in young subjects are not affected by an EPA intake of < or =4.05 g/d. Older subjects are more sensitive to the immunologic effects of EPA, and the neutrophil respiratory burst is lower at higher EPA intakes

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Published date: February 2006
Keywords: acid, acids, administration & dosage, adolescent, adult, aged, aging, analysis of variance, biosynthesis, blood, chemistry, comparative study, cytokines, design, dietary supplements, dinoprostone, dose-response relationship, drug, double-blind method, drug effects, eicosapentaenoic acid, fatty acid, fatty acids, human, humans, immunity, natural, immunology, leukocytes, mononuclear, male, metabolism, middle aged, neutrophils, phagocytosis, pharmacology, phospholipids, physiology, plasma, respiratory burst

Identifiers

Local EPrints ID: 44219
URI: http://eprints.soton.ac.uk/id/eprint/44219
ISSN: 0002-9165
PURE UUID: 05d4f710-96d3-4272-a957-e21b0e01049f
ORCID for Elizabeth A. Miles: ORCID iD orcid.org/0000-0002-8643-0655
ORCID for Philip C. Calder: ORCID iD orcid.org/0000-0002-6038-710X

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Date deposited: 20 Feb 2007
Last modified: 16 Mar 2024 02:51

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Contributors

Author: Dinka Rees
Author: Tapati Banerjee
Author: Solenne J. Wells
Author: Catherine E. Roynette
Author: Klaus W.J. Wahle

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