READ ME File For 'Keable_thesis_dataset_basement_membrane_analysis_of_cerebrovascular_cells'

Dataset DOI: 10.5258/SOTON/D1469

ReadMe Author: ABBY KEABLE, University of Southampton

This dataset supports the thesis entitled: An in vitro model of intramural periarterial drainage. Significance for Alzheimer's diease. 
AWARDED BY: University of Southampton
DATE OF AWARD: 2020

DESCRIPTION OF THE DATA
Data was obtained by imaging in vitro cell cultures immunostained for proteins of interest using a confocal microscope. 
Images were exported to ImageJ and either threshold analysis or RGB measure tools were used to quantify the amount of staining present.
Three images per experiment were captured and analysed and an average value per experiment was calculated.
Raw values and averages were recorded in microsoft Excel.
MTS absorbance was measured at 490nm with a Fluorstar optima plate reader and results were exported direct to Microsoft Excel with blank values subtracted.
Raw data is presented in a spreadsheet and is best viewed with Microsoft Excel



This dataset contains:

Threshold analysis of basement membrane immunostaining in ApoE astrocytes after 72 hours in culture and at 100% confluence
Fluorescence intensity values obtained from ImageJ RGB measure for basement membrane immunostaining in cerebrovascular cells at 72 hours
Manual cell count opf astrocytes and their processes obtained from scanning electron micrographs of ApoE astrocytes
Fluorescence intensity values obtained from ImageJ RGB measure for basement membrane immunostaining in cerebrovascular smooth muscle cells exposed to hypoxia and amyloid beta
Fluorescence intensity values obtained from ImageJ RGB measure for basement membrane immunostaining in cerebrovascular smooth muscle cells exposed to millifluidic flow of amyloid beta
MTS absorbance values for smooth muscle cell cultures exposed to hypoxia and amyloid beta at various timepoints
Fluorescence intensity values obtained from ImageJ RGB measure for adrenergic receptor immunostaining in contractile brain cells at 72 hours

Related projects/Funders:
BBSRC

Licence: CC BY https://creativecommons.org/licenses/by/4.0/

Date that the file was created: July, 2020