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Quantitative and correlative extreme ultraviolet coherent imaging of mouse hippocampal neurons at high resolution: Extreme ultraviolet coherent imaging of neurons

Quantitative and correlative extreme ultraviolet coherent imaging of mouse hippocampal neurons at high resolution: Extreme ultraviolet coherent imaging of neurons
Quantitative and correlative extreme ultraviolet coherent imaging of mouse hippocampal neurons at high resolution: Extreme ultraviolet coherent imaging of neurons

Microscopy with extreme ultraviolet (EUV) light can provide many advantages over optical, hard x-ray or electron-based techniques. However, traditional EUV sources and optics have large disadvantages of scale and cost. Here, we demonstrate the use of a laboratory-scale, coherent EUV source to image biological samples-mouse hippocampal neurons-providing quantitative phase and amplitude transmission information with a lateral resolution of 80 nm and an axial sensitivity of ~1 nm. A comparison with fluorescence imaging of the same samples demonstrated EUV imaging was able to identify, without the need for staining or superresolution techniques, <100-nm-wide and <10-nm-thick structures not observable from the fluorescence images. Unlike hard x-ray microscopy, no damage is observed of the delicate neuron structure. The combination of previously demonstrated tomographic imaging techniques with the latest advances in laser technologies and coherent EUV sources has the potential for high-resolution element-specific imaging within biological structures in 3D.

2375-2548
Baksh, Peter
578fea83-9a1c-4dd0-b3f6-66f5552f2344
Odstrčil, Michal
b297d3ec-ed42-4709-9f90-7af79d0644c7
Miszczak, Magdalena
7aeee058-080d-498a-9833-37a0dd8c60b0
Pooley, Charles
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Chapman, Richard
b696a894-6d7e-4ada-b9f9-994381a7d035
Wyatt, Adam
e439e99f-0f0c-41d6-9db4-a5ed09c9cd4b
Springate, Emma
f3004731-cf69-48bc-9816-1300a2276191
Chad, John
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Brocklesby, William
c53ca2f6-db65-4e19-ad00-eebeb2e6de67
Baksh, Peter
578fea83-9a1c-4dd0-b3f6-66f5552f2344
Odstrčil, Michal
b297d3ec-ed42-4709-9f90-7af79d0644c7
Miszczak, Magdalena
7aeee058-080d-498a-9833-37a0dd8c60b0
Pooley, Charles
62b2934a-6965-44e1-be45-b4e80e00439d
Chapman, Richard
b696a894-6d7e-4ada-b9f9-994381a7d035
Wyatt, Adam
e439e99f-0f0c-41d6-9db4-a5ed09c9cd4b
Springate, Emma
f3004731-cf69-48bc-9816-1300a2276191
Chad, John
d220e55e-3c13-4d1d-ae9a-1cfae8ccfbe1
Deinhardt, Katrin
5f4fe23b-2317-499f-ba6d-e639a4885dc1
Frey, Jeremy G.
ba60c559-c4af-44f1-87e6-ce69819bf23f
Brocklesby, William
c53ca2f6-db65-4e19-ad00-eebeb2e6de67

Baksh, Peter, Odstrčil, Michal, Miszczak, Magdalena, Pooley, Charles, Chapman, Richard, Wyatt, Adam, Springate, Emma, Chad, John, Deinhardt, Katrin, Frey, Jeremy G. and Brocklesby, William (2020) Quantitative and correlative extreme ultraviolet coherent imaging of mouse hippocampal neurons at high resolution: Extreme ultraviolet coherent imaging of neurons. Science Advances, 6 (18), [eaaz3025]. (doi:10.1126/sciadv.aaz3025).

Record type: Article

Abstract

Microscopy with extreme ultraviolet (EUV) light can provide many advantages over optical, hard x-ray or electron-based techniques. However, traditional EUV sources and optics have large disadvantages of scale and cost. Here, we demonstrate the use of a laboratory-scale, coherent EUV source to image biological samples-mouse hippocampal neurons-providing quantitative phase and amplitude transmission information with a lateral resolution of 80 nm and an axial sensitivity of ~1 nm. A comparison with fluorescence imaging of the same samples demonstrated EUV imaging was able to identify, without the need for staining or superresolution techniques, <100-nm-wide and <10-nm-thick structures not observable from the fluorescence images. Unlike hard x-ray microscopy, no damage is observed of the delicate neuron structure. The combination of previously demonstrated tomographic imaging techniques with the latest advances in laser technologies and coherent EUV sources has the potential for high-resolution element-specific imaging within biological structures in 3D.

Full text not available from this repository.

More information

Accepted/In Press date: 10 February 2020
e-pub ahead of print date: 1 May 2020
Published date: 1 May 2020

Identifiers

Local EPrints ID: 442555
URI: http://eprints.soton.ac.uk/id/eprint/442555
ISSN: 2375-2548
PURE UUID: 7fc90404-72f5-4c2a-9df0-71dcbe8a719c
ORCID for John Chad: ORCID iD orcid.org/0000-0001-6442-4281
ORCID for Katrin Deinhardt: ORCID iD orcid.org/0000-0002-6473-5298
ORCID for Jeremy G. Frey: ORCID iD orcid.org/0000-0003-0842-4302
ORCID for William Brocklesby: ORCID iD orcid.org/0000-0002-2123-6712

Catalogue record

Date deposited: 17 Jul 2020 16:36
Last modified: 15 Sep 2021 02:00

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Contributors

Author: Peter Baksh
Author: Michal Odstrčil
Author: Magdalena Miszczak
Author: Charles Pooley
Author: Richard Chapman
Author: Adam Wyatt
Author: Emma Springate
Author: John Chad ORCID iD
Author: Jeremy G. Frey ORCID iD

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