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Surfactant protein D reduces alveolar macrophage apoptosis In vivo

Surfactant protein D reduces alveolar macrophage apoptosis In vivo
Surfactant protein D reduces alveolar macrophage apoptosis In vivo
Surfactant protein D (SP-D) is a molecule of the innate immune system that recognizes the patterns of surface carbohydrate on pathogens and targets them for phagocytosis and killing. SP-D-deficient mice show an increased number of macrophages in the alveolar space, excess surfactant phospholipid, overproduction of reactive oxygen species, and the development of emphysema. We report here that SP-D-deficient mice have a 5- to 10-fold increase in the number of apoptotic and necrotic alveolar macrophages, as defined by annexin V and propidium iodine staining, respectively. Intrapulmonary administration of a truncated 60-kDa fragment of human recombinant SP-D reduces the number of apoptotic and necrotic alveolar macrophages and partially corrects the lipid accumulation in SP-D-deficient mice. The same SP-D fragment binds preferentially to apoptotic and necrotic alveolar macrophages in vitro, suggesting that SP-D contributes to immune homeostasis in the lung by recognizing and promoting removal of necrotic and apoptotic cells.
0022-1767
2892-2899
Clark, Howard
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Palaniyar, Nades
262890ae-b2b1-4f9b-8129-08f8bcd6a399
Strong, Peter
3b2156c4-3473-4c64-b8d6-be3362dd8a07
Edmondson, Jess
4f1ce923-b82e-4da1-9d44-3e2289646ba2
Hawgood, Samuel
5e406d87-8445-448a-a068-b693d061d6dd
Reid, Kenneth B.M.
74c6c3e3-d682-446d-b159-b635e6056e4e
Clark, Howard
70550b6d-3bd7-47c6-8c02-4f43f37d5213
Palaniyar, Nades
262890ae-b2b1-4f9b-8129-08f8bcd6a399
Strong, Peter
3b2156c4-3473-4c64-b8d6-be3362dd8a07
Edmondson, Jess
4f1ce923-b82e-4da1-9d44-3e2289646ba2
Hawgood, Samuel
5e406d87-8445-448a-a068-b693d061d6dd
Reid, Kenneth B.M.
74c6c3e3-d682-446d-b159-b635e6056e4e

Clark, Howard, Palaniyar, Nades, Strong, Peter, Edmondson, Jess, Hawgood, Samuel and Reid, Kenneth B.M. (2002) Surfactant protein D reduces alveolar macrophage apoptosis In vivo. The Journal of Immunology, 169 (6), 2892-2899.

Record type: Article

Abstract

Surfactant protein D (SP-D) is a molecule of the innate immune system that recognizes the patterns of surface carbohydrate on pathogens and targets them for phagocytosis and killing. SP-D-deficient mice show an increased number of macrophages in the alveolar space, excess surfactant phospholipid, overproduction of reactive oxygen species, and the development of emphysema. We report here that SP-D-deficient mice have a 5- to 10-fold increase in the number of apoptotic and necrotic alveolar macrophages, as defined by annexin V and propidium iodine staining, respectively. Intrapulmonary administration of a truncated 60-kDa fragment of human recombinant SP-D reduces the number of apoptotic and necrotic alveolar macrophages and partially corrects the lipid accumulation in SP-D-deficient mice. The same SP-D fragment binds preferentially to apoptotic and necrotic alveolar macrophages in vitro, suggesting that SP-D contributes to immune homeostasis in the lung by recognizing and promoting removal of necrotic and apoptotic cells.

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Published date: 15 September 2002

Identifiers

Local EPrints ID: 44261
URI: http://eprints.soton.ac.uk/id/eprint/44261
ISSN: 0022-1767
PURE UUID: c49a895e-f15e-4578-bf1b-73382f843722

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Date deposited: 21 Feb 2007
Last modified: 09 Dec 2019 19:06

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Contributors

Author: Howard Clark
Author: Nades Palaniyar
Author: Peter Strong
Author: Jess Edmondson
Author: Samuel Hawgood
Author: Kenneth B.M. Reid

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