Application of denaturing gradient gel electrophoresis (DGGE) in microbial ecology

Webb, Jeremy, Yuexin, Ma, Homstrm, Carola and Kjelleberg, Staffan (2003) Application of denaturing gradient gel electrophoresis (DGGE) in microbial ecology. Acta Ecologica Sinica, 23 (8), 1561-1569.

Abstract

Because of the difficulty associated with isolating and culturing bacteria from environmental samples, alternative methods based on molecular techniques have been developed to describe and identmicrobial communities. Recent years have witnessed a rapid development of DNA-based methods for community analysis such as PCR amplification, clone libraries, fluorescent in-situ hybridisation,restriction fragment length polymorphism，denaturing and temperature gradient gel electrophoresis. DGGE has been widely used in analyzing the biodiversity of bacterial, cyanobacterial, archaeal,picoeukaryotic, eukaryotic and viral communities in natural habitats. This technique can provide information on the predominant species in a community and analyze multiple samples simultaneously. The reproducibility and ease-of use of this technique perm it investigation of the spatial and temporal variability of the population and identification of community m em hers by sequencing of excised bands or by hybridization analysis with specific probes.

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Contributors

Author: Jeremy Webb

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