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Application of denaturing gradient gel electrophoresis (DGGE) in microbial ecology

Application of denaturing gradient gel electrophoresis (DGGE) in microbial ecology
Application of denaturing gradient gel electrophoresis (DGGE) in microbial ecology
Because of the difficulty associated with isolating and culturing bacteria from environmental samples, alternative methods based on molecular techniques have been developed to describe and identmicrobial communities. Recent years have witnessed a rapid development of DNA-based methods for community analysis such as PCR amplification, clone libraries, fluorescent in-situ hybridisation,restriction fragment length polymorphism,denaturing and temperature gradient gel electrophoresis. DGGE has been widely used in analyzing the biodiversity of bacterial, cyanobacterial, archaeal,picoeukaryotic, eukaryotic and viral communities in natural habitats. This technique can provide information on the predominant species in a community and analyze multiple samples simultaneously. The reproducibility and ease-of use of this technique perm it investigation of the spatial and temporal variability of the population and identification of community m em hers by sequencing of excised bands or by hybridization analysis with specific probes.
1561-1569
Webb, Jeremy
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Yuexin, Ma
b3d3513a-1c67-4d00-a3f6-8a9603e92d71
Homstrm, Carola
36ea2573-8b22-4149-aff7-7759d55bfd44
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e
Webb, Jeremy
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Yuexin, Ma
b3d3513a-1c67-4d00-a3f6-8a9603e92d71
Homstrm, Carola
36ea2573-8b22-4149-aff7-7759d55bfd44
Kjelleberg, Staffan
043b66b5-130c-42f2-99b3-ec3eecf3248e

Webb, Jeremy, Yuexin, Ma, Homstrm, Carola and Kjelleberg, Staffan (2003) Application of denaturing gradient gel electrophoresis (DGGE) in microbial ecology. Acta Ecologica Sinica, 23 (8), 1561-1569.

Record type: Article

Abstract

Because of the difficulty associated with isolating and culturing bacteria from environmental samples, alternative methods based on molecular techniques have been developed to describe and identmicrobial communities. Recent years have witnessed a rapid development of DNA-based methods for community analysis such as PCR amplification, clone libraries, fluorescent in-situ hybridisation,restriction fragment length polymorphism,denaturing and temperature gradient gel electrophoresis. DGGE has been widely used in analyzing the biodiversity of bacterial, cyanobacterial, archaeal,picoeukaryotic, eukaryotic and viral communities in natural habitats. This technique can provide information on the predominant species in a community and analyze multiple samples simultaneously. The reproducibility and ease-of use of this technique perm it investigation of the spatial and temporal variability of the population and identification of community m em hers by sequencing of excised bands or by hybridization analysis with specific probes.

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Published date: 1 January 2003

Identifiers

Local EPrints ID: 442644
URI: http://eprints.soton.ac.uk/id/eprint/442644
PURE UUID: 61b44157-6f45-4dd0-a2cb-4a64c06870ea
ORCID for Jeremy Webb: ORCID iD orcid.org/0000-0003-2068-8589

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Date deposited: 22 Jul 2020 16:31
Last modified: 18 Feb 2021 17:08

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