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Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth

Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth
Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth
Epidemic Pseudomonas aeruginosa have been identified in cystic fibrosis (CF) patients worldwide. The Australian Epidemic Strain-2 (AES-2) infects up to 40% of patients in three eastern Australian CF clinics. To investigate whether AES-2 isolates from chronically infected CF adults differentially express well-conserved genes potentially associated with transmissibility, we compared the transcriptomes of planktonic and biofilm-grown AES-2, infrequent P. aeruginosa clones and the reference P. aeruginosa PAO1 using the Affymetrix PAO1 array. The most interesting findings emerged from comparisons of planktonic and biofilm AES-2. AES-2 biofilms upregulated Type III secretion system genes, but downregulated quorum-sensing (QS)-regulatory genes, except lasR, QS-regulated, oxidative-stress and iron-storage genes. QS-regulated and iron-storage genes were downregulated to a greater extent in AES-2 biofilms compared with infrequent clone and PAO1 biofilms, suggesting enhanced anaerobic respiration in AES-2. Chitinase and chitin-binding protein maintained high expression in AES-2 biofilms compared with infrequent clone and PAO1 biofilms. Planktonic AES-2 upregulated QS regulators and QS-regulated genes, iron acquisition and aerobic respiration genes, and had high expression of Group III Type IV pilA compared with low expression of Group I Type IV pilA in infrequent clones. Together, these properties may enhance long-term survival of AES-2 in CF lung and contribute to its transmissibility.
0378-1097
107-114
Webb, Jeremy
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Manos, Jim
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Arthur, Jonathan
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Rose, Barbara
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Bell, Scott
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Tingpej, Pholawat
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Hu, Honghua
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Kjelleberg, Staffan
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Gorrell, Mark D.
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Bye, Peter
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Harbour, Colin
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Webb, Jeremy
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Manos, Jim
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Arthur, Jonathan
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Rose, Barbara
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Bell, Scott
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Tingpej, Pholawat
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Hu, Honghua
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Kjelleberg, Staffan
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Gorrell, Mark D.
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Bye, Peter
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Harbour, Colin
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Webb, Jeremy, Manos, Jim, Arthur, Jonathan, Rose, Barbara, Bell, Scott, Tingpej, Pholawat, Hu, Honghua, Kjelleberg, Staffan, Gorrell, Mark D., Bye, Peter and Harbour, Colin (2009) Gene expression characteristics of a cystic fibrosis epidemic strain of Pseudomonas aeruginosa during biofilm and planktonic growth. FEMS Microbiology Letters, 292 (1), 107-114. (doi:10.1111/j.1574-6968.2008.01472.x).

Record type: Article

Abstract

Epidemic Pseudomonas aeruginosa have been identified in cystic fibrosis (CF) patients worldwide. The Australian Epidemic Strain-2 (AES-2) infects up to 40% of patients in three eastern Australian CF clinics. To investigate whether AES-2 isolates from chronically infected CF adults differentially express well-conserved genes potentially associated with transmissibility, we compared the transcriptomes of planktonic and biofilm-grown AES-2, infrequent P. aeruginosa clones and the reference P. aeruginosa PAO1 using the Affymetrix PAO1 array. The most interesting findings emerged from comparisons of planktonic and biofilm AES-2. AES-2 biofilms upregulated Type III secretion system genes, but downregulated quorum-sensing (QS)-regulatory genes, except lasR, QS-regulated, oxidative-stress and iron-storage genes. QS-regulated and iron-storage genes were downregulated to a greater extent in AES-2 biofilms compared with infrequent clone and PAO1 biofilms, suggesting enhanced anaerobic respiration in AES-2. Chitinase and chitin-binding protein maintained high expression in AES-2 biofilms compared with infrequent clone and PAO1 biofilms. Planktonic AES-2 upregulated QS regulators and QS-regulated genes, iron acquisition and aerobic respiration genes, and had high expression of Group III Type IV pilA compared with low expression of Group I Type IV pilA in infrequent clones. Together, these properties may enhance long-term survival of AES-2 in CF lung and contribute to its transmissibility.

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Accepted/In Press date: 8 December 2008
Published date: 1 March 2009

Identifiers

Local EPrints ID: 442651
URI: http://eprints.soton.ac.uk/id/eprint/442651
ISSN: 0378-1097
PURE UUID: 5ba2e992-e7a8-4382-8ebb-1733c76ab241
ORCID for Jeremy Webb: ORCID iD orcid.org/0000-0003-2068-8589

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Date deposited: 22 Jul 2020 16:31
Last modified: 18 Feb 2021 17:08

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Contributors

Author: Jeremy Webb ORCID iD
Author: Jim Manos
Author: Jonathan Arthur
Author: Barbara Rose
Author: Scott Bell
Author: Pholawat Tingpej
Author: Honghua Hu
Author: Staffan Kjelleberg
Author: Mark D. Gorrell
Author: Peter Bye
Author: Colin Harbour

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