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Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease

Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease
Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease
Background: the number of type 2 helper CD4+ T cells is increased in the airways of persons with asthma. Whether the majority of these cells are class II major-histocompatibility-complex-restricted cells or are among the recently identified CD1d-restricted invariant natural killer T cells is a matter of controversy. We studied the frequency of invariant natural killer T cells in the airways of subjects with mild or moderately severe asthma to investigate the possibility of an association between the number of invariant natural killer T cells in the airway and disease severity. We also studied whether an increased number of these cells is a feature of chronic obstructive pulmonary disease (COPD).

Methods: we enumerated invariant natural killer T cells by flow cytometry with the use of CD1d tetramers loaded with alpha-galactosylceramide and antibodies specific to the invariant natural killer T-cell receptor in samples of bronchoalveolar-lavage fluid, induced sputum, and bronchial-biopsy specimens obtained from subjects with mild or moderately severe asthma, subjects with COPD, and healthy control subjects. Real-time polymerase-chain-reaction analysis was performed on bronchoalveolar-lavage cells for evidence of gene expression of the invariant natural killer T-cell receptor.

Results: fewer than 2% of the T cells obtained from all subjects on airway biopsy, bronchoalveolar lavage, and sputum induction were invariant natural killer T cells, with no significant differences among the three groups of subjects. No expression of messenger RNA for the invariant natural killer T-cell-receptor domains Valpha24 and Vbeta11 was detected in bronchoalveolar-lavage cells from subjects with asthma.

Conclusions: invariant natural killer T cells are found in low numbers in the airways of subjects with asthma, subjects with COPD, and controls.
1410-1422
Vijayanand, Pandurangan
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Seumois, Grégory
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Pickard, Chris
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Powell, Robert M.
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Angco, Gilbert
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Sammut, David
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Gadola, Stephen D.
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Friedmann, Peter S.
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Djukanovic, Ratko
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Vijayanand, Pandurangan
79514f33-66cf-47cc-a8fa-46bbfc21b7d1
Seumois, Grégory
7cf4a076-ecd9-4e0b-8f6b-a7ae3c76ce65
Pickard, Chris
d243fbd8-ea2c-4245-b64a-56b61a4f4d03
Powell, Robert M.
884d6594-3f50-4be5-9516-d64b29dad63d
Angco, Gilbert
8f4d5efe-fa0c-4953-b255-3d221bcd16ab
Sammut, David
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Gadola, Stephen D.
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Friedmann, Peter S.
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Djukanovic, Ratko
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Vijayanand, Pandurangan, Seumois, Grégory, Pickard, Chris, Powell, Robert M., Angco, Gilbert, Sammut, David, Gadola, Stephen D., Friedmann, Peter S. and Djukanovic, Ratko (2007) Invariant natural killer T cells in asthma and chronic obstructive pulmonary disease. New England Journal of Medicine, 356 (14), 1410-1422. (doi:10.1056/NEJMoa064691).

Record type: Article

Abstract

Background: the number of type 2 helper CD4+ T cells is increased in the airways of persons with asthma. Whether the majority of these cells are class II major-histocompatibility-complex-restricted cells or are among the recently identified CD1d-restricted invariant natural killer T cells is a matter of controversy. We studied the frequency of invariant natural killer T cells in the airways of subjects with mild or moderately severe asthma to investigate the possibility of an association between the number of invariant natural killer T cells in the airway and disease severity. We also studied whether an increased number of these cells is a feature of chronic obstructive pulmonary disease (COPD).

Methods: we enumerated invariant natural killer T cells by flow cytometry with the use of CD1d tetramers loaded with alpha-galactosylceramide and antibodies specific to the invariant natural killer T-cell receptor in samples of bronchoalveolar-lavage fluid, induced sputum, and bronchial-biopsy specimens obtained from subjects with mild or moderately severe asthma, subjects with COPD, and healthy control subjects. Real-time polymerase-chain-reaction analysis was performed on bronchoalveolar-lavage cells for evidence of gene expression of the invariant natural killer T-cell receptor.

Results: fewer than 2% of the T cells obtained from all subjects on airway biopsy, bronchoalveolar lavage, and sputum induction were invariant natural killer T cells, with no significant differences among the three groups of subjects. No expression of messenger RNA for the invariant natural killer T-cell-receptor domains Valpha24 and Vbeta11 was detected in bronchoalveolar-lavage cells from subjects with asthma.

Conclusions: invariant natural killer T cells are found in low numbers in the airways of subjects with asthma, subjects with COPD, and controls.

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Published date: 5 April 2007

Identifiers

Local EPrints ID: 44275
URI: http://eprints.soton.ac.uk/id/eprint/44275
PURE UUID: db877e95-4663-4568-840c-aeb6bcb6a4a0
ORCID for Ratko Djukanovic: ORCID iD orcid.org/0000-0001-6039-5612

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Date deposited: 16 Apr 2007
Last modified: 17 Dec 2019 02:03

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Contributors

Author: Pandurangan Vijayanand
Author: Grégory Seumois
Author: Chris Pickard
Author: Robert M. Powell
Author: Gilbert Angco
Author: David Sammut
Author: Stephen D. Gadola
Author: Peter S. Friedmann

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