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The genetics and genomics of the drought response in Populus

Record type: Article

The genetic nature of tree adaptation to drought stress was examined by utilizing variation in the drought response of a full-sib second generation (F2) mapping population from a cross between Populus trichocarpa (93-968) and P. deltoides Bart (ILL-129) and known to be highly divergent for a vast range of phenotypic traits. We combined phenotyping, quantitative trait loci (QTL) analysis and microarray experiments to demonstrate that ‘genetical genomics’ can be used to provide information on adaptation at the species level. The grandparents and F2 population were subjected to soil drying, and contrasting responses to drought across genotypes, including leaf coloration, expansion and abscission, were observed, and QTL for these traits mapped. A subset of extreme genotypes exhibiting extreme sensitivity and insensitivity to drought on the basis of leaf abscission were defined, and microarray experiments conducted on these genotypes and the grandparent species. The extreme genotype groups induced a different set of genes: 215 and 125 genes differed in their expression response between groups in control and drought, respectively, suggesting species adaptation at the gene expression level. Co-location of differentially expressed genes with drought-specific and drought-responsive QTLs was examined, and these may represent candidate genes contributing to the variation in drought response.

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Citation

Street, Nathaniel Robert, Skogström, Oskar, Sjödin, Andreas, Tucker, James, Rodriguez-Acosta, Maricela, Nisson, Peter, Jansson, Stefan and Taylor, Gail (2006) The genetics and genomics of the drought response in Populus Plant Journal, 48, (3), pp. 321-341. (doi:10.1111/j.1365-313X.2006.02864.x).

More information

Published date: 2006
Keywords: quantitative trait loci, pooling messenger-rna, tvichocavpa cv peace, microarray experiments, hybrid poplar, water-stress cdna microarray, physiological traits, arabidopsis-thaliana, riparian cottonwoods

Identifiers

Local EPrints ID: 44282
URI: http://eprints.soton.ac.uk/id/eprint/44282
ISSN: 0960-7412
PURE UUID: 02f8f939-76b0-4b34-9cb3-2cc90a43e3e8

Catalogue record

Date deposited: 22 Feb 2007
Last modified: 17 Jul 2017 15:15

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Contributors

Author: Nathaniel Robert Street
Author: Oskar Skogström
Author: Andreas Sjödin
Author: James Tucker
Author: Maricela Rodriguez-Acosta
Author: Peter Nisson
Author: Stefan Jansson
Author: Gail Taylor

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