The University of Southampton
University of Southampton Institutional Repository
Warning ePrints Soton is experiencing an issue with some file downloads not being available. We are working hard to fix this. Please bear with us.

A Systematic Approach To Review of In vitro methods in Brain Tumour Research (SAToRI-BTR): development of a preliminary checklist for evaluating quality and human relevance

A Systematic Approach To Review of In vitro methods in Brain Tumour Research (SAToRI-BTR): development of a preliminary checklist for evaluating quality and human relevance
A Systematic Approach To Review of In vitro methods in Brain Tumour Research (SAToRI-BTR): development of a preliminary checklist for evaluating quality and human relevance
Background - A wide range of human in vitro methods have been developed and there is considerable interest in the potential of these studies to address questions related to clinical (human) use of drugs, and the pathobiology of tumours. This requires agreement on how to assess the strength of evidence available (i.e. quality and quantity) and the human-relevance of such studies. The SAToRI-BTR (Systematic Approach To Review of In vitro methods in Brain Tumour Research) project seeks to identify relevant appraisal criteria to aid planning and/or evaluation of brain tumour studies using in vitro methods.

Objectives – To identify criteria for evaluation of quality and human relevance of in vitro brain tumour studies; to assess the general acceptability of such criteria to senior scientists working within the field.

Methods – Stage one involved identification of potential criteria for evaluation of in vitro studies through: (1) an international survey of brain tumour researchers; (2) interviews with scientists, clinicians, regulators, and journal editors; (3) analysis of relevant reports, documents, and published studies. Through content analysis of findings, an initial list of criteria for quality appraisal of in vitro studies of brain tumours was developed. Stage two involved review of the criteria by an expert panel (Delphi process).

Results – Results of stage one indicated that methods for and quality of review of in vitro studies are highly variable, and that improved reporting standards are needed. 129 preliminary criteria were identified; duplicate and highly context-specific items were removed, resulting in 48 criteria for review by the expert (Delphi) panel. 37 criteria reached agreement, resulting in a provisional checklist for appraisal of in vitro studies in brain tumour research.

Conclusion – Through a systematic process of collating assessment criteria and subjecting these to expert review, SAToRI-BTR has resulted in preliminary guidance for appraisal of in vitro brain tumour studies. Further development of this guidance, including investigating strategies for adaptation and dissemination across different sub-fields of brain tumour research, as well as the wider in vitro field, is planned.

brain tumour, cancer, critical appraisal, evaluation, in vitro, quality appraisal, systematic review
1-17
Bracher, Michael
e9e2fbd6-af5f-4f6e-8357-969aaf51c52e
Pilkington, Geoffrey J.
c61b55d2-41d3-46e9-96ee-48a82e1abb0d
Hanemann, C O
5c270618-48cd-49ee-bf72-bbc828a35895
Pilkington, Karen
2abedd15-16bf-45df-a21c-82820fea5743
Bracher, Michael
e9e2fbd6-af5f-4f6e-8357-969aaf51c52e
Pilkington, Geoffrey J.
c61b55d2-41d3-46e9-96ee-48a82e1abb0d
Hanemann, C O
5c270618-48cd-49ee-bf72-bbc828a35895
Pilkington, Karen
2abedd15-16bf-45df-a21c-82820fea5743

Bracher, Michael, Pilkington, Geoffrey J., Hanemann, C O and Pilkington, Karen (2020) A Systematic Approach To Review of In vitro methods in Brain Tumour Research (SAToRI-BTR): development of a preliminary checklist for evaluating quality and human relevance. Frontiers in Bioengineering and Biotechnology, 8, 1-17, [936]. (doi:10.3389/fbioe.2020.00936).

Record type: Article

Abstract

Background - A wide range of human in vitro methods have been developed and there is considerable interest in the potential of these studies to address questions related to clinical (human) use of drugs, and the pathobiology of tumours. This requires agreement on how to assess the strength of evidence available (i.e. quality and quantity) and the human-relevance of such studies. The SAToRI-BTR (Systematic Approach To Review of In vitro methods in Brain Tumour Research) project seeks to identify relevant appraisal criteria to aid planning and/or evaluation of brain tumour studies using in vitro methods.

Objectives – To identify criteria for evaluation of quality and human relevance of in vitro brain tumour studies; to assess the general acceptability of such criteria to senior scientists working within the field.

Methods – Stage one involved identification of potential criteria for evaluation of in vitro studies through: (1) an international survey of brain tumour researchers; (2) interviews with scientists, clinicians, regulators, and journal editors; (3) analysis of relevant reports, documents, and published studies. Through content analysis of findings, an initial list of criteria for quality appraisal of in vitro studies of brain tumours was developed. Stage two involved review of the criteria by an expert panel (Delphi process).

Results – Results of stage one indicated that methods for and quality of review of in vitro studies are highly variable, and that improved reporting standards are needed. 129 preliminary criteria were identified; duplicate and highly context-specific items were removed, resulting in 48 criteria for review by the expert (Delphi) panel. 37 criteria reached agreement, resulting in a provisional checklist for appraisal of in vitro studies in brain tumour research.

Conclusion – Through a systematic process of collating assessment criteria and subjecting these to expert review, SAToRI-BTR has resulted in preliminary guidance for appraisal of in vitro brain tumour studies. Further development of this guidance, including investigating strategies for adaptation and dissemination across different sub-fields of brain tumour research, as well as the wider in vitro field, is planned.

Other
A Systematic Approach To Review of In vitro methods in Brain Tumour Research (SAToRI-BTR) development of a preliminary checklist for evaluating quality and human relevance - Accepted Manuscript
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 20 July 2020
e-pub ahead of print date: 7 August 2020
Keywords: brain tumour, cancer, critical appraisal, evaluation, in vitro, quality appraisal, systematic review

Identifiers

Local EPrints ID: 442885
URI: http://eprints.soton.ac.uk/id/eprint/442885
PURE UUID: 83e9181d-4ef2-4198-9568-10ff2d8c5994
ORCID for Michael Bracher: ORCID iD orcid.org/0000-0001-5861-2657

Catalogue record

Date deposited: 30 Jul 2020 16:30
Last modified: 26 Nov 2021 02:55

Export record

Altmetrics

Contributors

Author: Michael Bracher ORCID iD
Author: Geoffrey J. Pilkington
Author: C O Hanemann
Author: Karen Pilkington

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×