The University of Southampton
University of Southampton Institutional Repository

Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins

Batten, Carrie A., Clarke, Ian N., Kempster, Sarah L., Oliver, Stefan L., Bridger, Janice C. and Lambden, Paul R. (2006) Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins Virology, 356, (1-2), pp. 179-187. (doi:10.1016/j.virol.2006.07.034). (PMID:16934306).

Record type: Article


The Southampton norovirus (SV) capsid protein was expressed as VLPs by recombinant baculoviruses in insect cells and was used to immunize mice for the production of monoclonal antibodies (mAbs). One mAb, CM54, showed broad cross-reactivity to genogroup I (GI) noroviruses, but was not reactive to GII capsid proteins. Interestingly mAb CM54 reacted to a bovine norovirus capsid protein. Immunoblot analysis indicated the binding site for CM54 was located in the shell domain between amino acid residues 102–225 of the SV capsid protein. The epitope was mapped to high resolution using a peptide array and was located to the sequence LEDVRN at amino acid residues 162–167. Alignment of norovirus capsid protein sequences confirmed the epitope sequence was common to particular groups of human and bovine noroviruses. Modeling of the epitope onto the recombinant NV capsid protein revealed it was located to the inner surface of the shell domain.

Full text not available from this repository.

More information

Published date: 2006
Keywords: norovirus, capsid, monoclonal antibody, epitope


Local EPrints ID: 44300
ISSN: 0042-6822
PURE UUID: 3249fd1f-ec72-45ab-8d02-db39e27e42b3
ORCID for Ian N. Clarke: ORCID iD

Catalogue record

Date deposited: 22 Feb 2007
Last modified: 17 Jul 2017 15:15

Export record



Author: Carrie A. Batten
Author: Ian N. Clarke ORCID iD
Author: Sarah L. Kempster
Author: Stefan L. Oliver
Author: Janice C. Bridger
Author: Paul R. Lambden

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.