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Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins

Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins
Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins
The Southampton norovirus (SV) capsid protein was expressed as VLPs by recombinant baculoviruses in insect cells and was used to immunize mice for the production of monoclonal antibodies (mAbs). One mAb, CM54, showed broad cross-reactivity to genogroup I (GI) noroviruses, but was not reactive to GII capsid proteins. Interestingly mAb CM54 reacted to a bovine norovirus capsid protein. Immunoblot analysis indicated the binding site for CM54 was located in the shell domain between amino acid residues 102–225 of the SV capsid protein. The epitope was mapped to high resolution using a peptide array and was located to the sequence LEDVRN at amino acid residues 162–167. Alignment of norovirus capsid protein sequences confirmed the epitope sequence was common to particular groups of human and bovine noroviruses. Modeling of the epitope onto the recombinant NV capsid protein revealed it was located to the inner surface of the shell domain.

norovirus, capsid, monoclonal antibody, epitope
0042-6822
179-187
Batten, Carrie A.
d8d8d96e-b954-4566-b737-b58d81fe49c5
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Kempster, Sarah L.
3e1f129d-73db-4226-bea6-dfd6efc66756
Oliver, Stefan L.
5efbfdd8-cec5-4a21-a5f7-8f05213bb22e
Bridger, Janice C.
4660b463-c316-4cbb-846a-37c7d64f1cda
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698
Batten, Carrie A.
d8d8d96e-b954-4566-b737-b58d81fe49c5
Clarke, Ian N.
ff6c9324-3547-4039-bb2c-10c0b3327a8b
Kempster, Sarah L.
3e1f129d-73db-4226-bea6-dfd6efc66756
Oliver, Stefan L.
5efbfdd8-cec5-4a21-a5f7-8f05213bb22e
Bridger, Janice C.
4660b463-c316-4cbb-846a-37c7d64f1cda
Lambden, Paul R.
4fcd536e-2d9a-4366-97c6-386e6b005698

Batten, Carrie A., Clarke, Ian N., Kempster, Sarah L., Oliver, Stefan L., Bridger, Janice C. and Lambden, Paul R. (2006) Characterization of a cross-reactive linear epitope in human genogroup I and bovine genogroup III norovirus capsid proteins. Virology, 356 (1-2), 179-187. (doi:10.1016/j.virol.2006.07.034). (PMID:16934306)

Record type: Article

Abstract

The Southampton norovirus (SV) capsid protein was expressed as VLPs by recombinant baculoviruses in insect cells and was used to immunize mice for the production of monoclonal antibodies (mAbs). One mAb, CM54, showed broad cross-reactivity to genogroup I (GI) noroviruses, but was not reactive to GII capsid proteins. Interestingly mAb CM54 reacted to a bovine norovirus capsid protein. Immunoblot analysis indicated the binding site for CM54 was located in the shell domain between amino acid residues 102–225 of the SV capsid protein. The epitope was mapped to high resolution using a peptide array and was located to the sequence LEDVRN at amino acid residues 162–167. Alignment of norovirus capsid protein sequences confirmed the epitope sequence was common to particular groups of human and bovine noroviruses. Modeling of the epitope onto the recombinant NV capsid protein revealed it was located to the inner surface of the shell domain.

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More information

Published date: 2006
Keywords: norovirus, capsid, monoclonal antibody, epitope

Identifiers

Local EPrints ID: 44300
URI: https://eprints.soton.ac.uk/id/eprint/44300
ISSN: 0042-6822
PURE UUID: 3249fd1f-ec72-45ab-8d02-db39e27e42b3
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 22 Feb 2007
Last modified: 14 Mar 2019 01:56

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