Keeping CRISPR in check: diverse mechanisms of phage-encoded anti-CRISPRs
Keeping CRISPR in check: diverse mechanisms of phage-encoded anti-CRISPRs
CRISPR-Cas represents the only adaptive immune system of prokaryotes known to date. These immune systems are widespread among bacteria and archaea, and provide protection against invasion of mobile genetic elements, such as bacteriophages and plasmids. As a result of the arms-race between phages and their prokaryotic hosts, phages have evolved inhibitors known as anti-CRISPR (Acr) proteins to evade CRISPR immunity. In the recent years, several Acr proteins have been described in both temperate and virulent phages targeting diverse CRISPR-Cas systems. Here, we describe the strategies of Acr discovery and the multiple molecular mechanisms by which these proteins operate to inhibit CRISPR immunity. We discuss the biological relevance of Acr proteins and speculate on the implications of their activity for the development of improved CRISPR-based research and biotechnological tools.
Trasanidov, Despoina
2153cb21-c344-4695-9bfd-6abdb54009f2
Gerós, Ana
bb6b5455-9cbc-4f15-8923-44c0dc9a520e
Mohanraju, Prarthana
f4b92ec0-3b3b-436a-a5f5-f55805408245
Nieuwenweg, Anna Cornelia
a5e0d923-8e3c-4de4-be09-843ea0af7f13
Luzia De Nobrega, Franklin
6532795d-88a4-4f05-9b26-6af5b8f21a0d
Staals, Raymond H J
4a2c653f-7baa-4a58-a482-6ed9266b3786
May 2019
Trasanidov, Despoina
2153cb21-c344-4695-9bfd-6abdb54009f2
Gerós, Ana
bb6b5455-9cbc-4f15-8923-44c0dc9a520e
Mohanraju, Prarthana
f4b92ec0-3b3b-436a-a5f5-f55805408245
Nieuwenweg, Anna Cornelia
a5e0d923-8e3c-4de4-be09-843ea0af7f13
Luzia De Nobrega, Franklin
6532795d-88a4-4f05-9b26-6af5b8f21a0d
Staals, Raymond H J
4a2c653f-7baa-4a58-a482-6ed9266b3786
Trasanidov, Despoina, Gerós, Ana, Mohanraju, Prarthana, Nieuwenweg, Anna Cornelia, Luzia De Nobrega, Franklin and Staals, Raymond H J
(2019)
Keeping CRISPR in check: diverse mechanisms of phage-encoded anti-CRISPRs.
FEMS Microbiology Letters, 366 (9), [fnz098].
(doi:10.1093/femsle/fnz098).
Abstract
CRISPR-Cas represents the only adaptive immune system of prokaryotes known to date. These immune systems are widespread among bacteria and archaea, and provide protection against invasion of mobile genetic elements, such as bacteriophages and plasmids. As a result of the arms-race between phages and their prokaryotic hosts, phages have evolved inhibitors known as anti-CRISPR (Acr) proteins to evade CRISPR immunity. In the recent years, several Acr proteins have been described in both temperate and virulent phages targeting diverse CRISPR-Cas systems. Here, we describe the strategies of Acr discovery and the multiple molecular mechanisms by which these proteins operate to inhibit CRISPR immunity. We discuss the biological relevance of Acr proteins and speculate on the implications of their activity for the development of improved CRISPR-based research and biotechnological tools.
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Accepted/In Press date: 10 May 2019
e-pub ahead of print date: 11 May 2019
Published date: May 2019
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Local EPrints ID: 443095
URI: http://eprints.soton.ac.uk/id/eprint/443095
ISSN: 0378-1097
PURE UUID: bad9a7d6-3f05-4495-a9e6-8ffca9f6d8ca
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Date deposited: 11 Aug 2020 16:30
Last modified: 17 Mar 2024 04:02
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Author:
Despoina Trasanidov
Author:
Ana Gerós
Author:
Prarthana Mohanraju
Author:
Anna Cornelia Nieuwenweg
Author:
Raymond H J Staals
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