Peripheral immunophenotype in Dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
Peripheral immunophenotype in Dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study
Background: Inflammation plays a key role in the aetiology and progression of Alzheimer’s disease (AD). However, the immunophenotype of the second most common neurodegenerative cause of dementia, dementia with Lewy bodies (DLB), remains unclear. To date there have been no studies examining peripheral inflammation in DLB using multiplex immunoassay and flow cytometry concomitantly. We hypothesised that, using blood biomarkers, DLB would show an increased proinflammatory profile compared with controls, and that there would be a distinct profile compared with AD.
Methods: 93 participants (31 with DLB, 31 with AD and 31 healthy older controls) completed a single study visit for neuropsychiatric testing and phlebotomy. Peripheral blood mononuclear cells were quantified for T and B cell subsets using flow cytometry, and serum cytokine concentrations were measured using multiplex immunoassay.
Results: We detected reduced relative numbers of helper T cells and reduced activation of B cells in DLB compared with AD. Additionally, interleukin (IL)-1β was detected more frequently in DLB and the serum concentration of IL-6 was increased compared with controls.
Conclusions: Peripheral inflammation is altered in DLB compared with AD, with T cell subset analysis supporting a possible shift towards senescence of the adaptive immune system in DLB. Furthermore, there is a proinflammatory signature of serum cytokines in DLB. Identification of this unique peripheral immunophenotype in DLB could guide development of an immune-based biomarker and direct future work exploring potential immune modulation as a novel treatment.
1219-1226
Amin, Jay
692a8880-70ff-4b64-a7e9-7d0d53449a30
Boche, Delphine
bdcca10e-6302-4dd0-919f-67218f7e0d61
Clough, Zoe
06b0b5fd-881d-4959-a048-2d006856990d
Teeling, Jessica
fcde1c8e-e5f8-4747-9f3a-6bdb5cd87d0a
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Gao, Yifang
eea234ba-f566-4f21-a65e-234b84cba285
Chudley, Lindsey
ec25c30c-369d-4516-b872-4cb3c135c10a
Lau, Laurie
2af8045d-6162-4939-aba7-28dd2f60f6a8
Smith, Florence
f6ab573b-07d0-4aa0-97a4-d2b366e75a36
Harris, Scott
19ea097b-df15-4f0f-be19-8ac42c190028
Holmes, Clive
ada5abf3-8459-4cf7-be40-3f4e9391cc96
November 2020
Amin, Jay
692a8880-70ff-4b64-a7e9-7d0d53449a30
Boche, Delphine
bdcca10e-6302-4dd0-919f-67218f7e0d61
Clough, Zoe
06b0b5fd-881d-4959-a048-2d006856990d
Teeling, Jessica
fcde1c8e-e5f8-4747-9f3a-6bdb5cd87d0a
Williams, Anthony
973ff46f-46f1-4d7c-b27d-0f53221e4c44
Gao, Yifang
eea234ba-f566-4f21-a65e-234b84cba285
Chudley, Lindsey
ec25c30c-369d-4516-b872-4cb3c135c10a
Lau, Laurie
2af8045d-6162-4939-aba7-28dd2f60f6a8
Smith, Florence
f6ab573b-07d0-4aa0-97a4-d2b366e75a36
Harris, Scott
19ea097b-df15-4f0f-be19-8ac42c190028
Holmes, Clive
ada5abf3-8459-4cf7-be40-3f4e9391cc96
Amin, Jay, Boche, Delphine, Clough, Zoe, Teeling, Jessica, Williams, Anthony, Gao, Yifang, Chudley, Lindsey, Lau, Laurie, Smith, Florence, Harris, Scott and Holmes, Clive
(2020)
Peripheral immunophenotype in Dementia with Lewy bodies and Alzheimer’s disease: an observational clinical study.
Journal of Neurology, Neurosurgery & Psychiatry, 91 (11), .
(doi:10.1136/jnnp-2020-323603).
Abstract
Background: Inflammation plays a key role in the aetiology and progression of Alzheimer’s disease (AD). However, the immunophenotype of the second most common neurodegenerative cause of dementia, dementia with Lewy bodies (DLB), remains unclear. To date there have been no studies examining peripheral inflammation in DLB using multiplex immunoassay and flow cytometry concomitantly. We hypothesised that, using blood biomarkers, DLB would show an increased proinflammatory profile compared with controls, and that there would be a distinct profile compared with AD.
Methods: 93 participants (31 with DLB, 31 with AD and 31 healthy older controls) completed a single study visit for neuropsychiatric testing and phlebotomy. Peripheral blood mononuclear cells were quantified for T and B cell subsets using flow cytometry, and serum cytokine concentrations were measured using multiplex immunoassay.
Results: We detected reduced relative numbers of helper T cells and reduced activation of B cells in DLB compared with AD. Additionally, interleukin (IL)-1β was detected more frequently in DLB and the serum concentration of IL-6 was increased compared with controls.
Conclusions: Peripheral inflammation is altered in DLB compared with AD, with T cell subset analysis supporting a possible shift towards senescence of the adaptive immune system in DLB. Furthermore, there is a proinflammatory signature of serum cytokines in DLB. Identification of this unique peripheral immunophenotype in DLB could guide development of an immune-based biomarker and direct future work exploring potential immune modulation as a novel treatment.
Text
Peripheral inflammation in DLB and AD
- Accepted Manuscript
More information
Accepted/In Press date: 30 July 2020
e-pub ahead of print date: 23 September 2020
Published date: November 2020
Additional Information:
© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY. Published by BMJ.
Identifiers
Local EPrints ID: 443115
URI: http://eprints.soton.ac.uk/id/eprint/443115
ISSN: 0022-3050
PURE UUID: 1983d497-1314-4fac-9eb5-a6ba7368c1a2
Catalogue record
Date deposited: 11 Aug 2020 16:31
Last modified: 30 Nov 2024 05:04
Export record
Altmetrics
Contributors
Author:
Zoe Clough
Author:
Yifang Gao
Author:
Lindsey Chudley
Author:
Laurie Lau
Author:
Florence Smith
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
