Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase
Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase
One electron reductions of oxygen and nitrite by xanthine oxidase form peroxynitrite. The nitrite and oxygen reducing activities of xanthine oxidase are regulated by oxygen with K(oxygen) 26 and 100 microM and K(nitrite) 1.0 and 1.1 mM with xanthine and NADH as donor substrates. Optimal peroxynitrite formation occurs at 70 microM oxygen with purine substrates. Kinetic parameters: V(max) approximately 50 nmol/min/mg and K(m) of 22, 36 and 70 microM for hypoxanthine, pterin and nitrite respectively. Peroxynitrite generation is inhibited by allopurinol, superoxide dismutase and diphenylene iodonium. A role for this enzyme activity can be found in the antibacterial activity of milk and circulating xanthine oxidase activity.
xanthine oxidase, peroxynitrite, nitric oxide, superoxide
129-133
Millar, Timothy M.
ec88510c-ad88-49f6-8b2d-4277c84c1958
2004
Millar, Timothy M.
ec88510c-ad88-49f6-8b2d-4277c84c1958
Millar, Timothy M.
(2004)
Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase.
FEBS Letters, 562 (1-3), .
(doi:10.1016/S0014-5793(04)00218-2).
Abstract
One electron reductions of oxygen and nitrite by xanthine oxidase form peroxynitrite. The nitrite and oxygen reducing activities of xanthine oxidase are regulated by oxygen with K(oxygen) 26 and 100 microM and K(nitrite) 1.0 and 1.1 mM with xanthine and NADH as donor substrates. Optimal peroxynitrite formation occurs at 70 microM oxygen with purine substrates. Kinetic parameters: V(max) approximately 50 nmol/min/mg and K(m) of 22, 36 and 70 microM for hypoxanthine, pterin and nitrite respectively. Peroxynitrite generation is inhibited by allopurinol, superoxide dismutase and diphenylene iodonium. A role for this enzyme activity can be found in the antibacterial activity of milk and circulating xanthine oxidase activity.
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Published date: 2004
Keywords:
xanthine oxidase, peroxynitrite, nitric oxide, superoxide
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Local EPrints ID: 44319
URI: http://eprints.soton.ac.uk/id/eprint/44319
ISSN: 0014-5793
PURE UUID: 606f051f-94d1-46dd-bc67-cc7975d4e75c
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Date deposited: 23 Feb 2007
Last modified: 15 Mar 2024 09:02
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Author:
Timothy M. Millar
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