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Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase

Millar, Timothy M. (2004) Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase Febs Letters, 562, (1-3), pp. 129-133. (doi:10.1016/S0014-5793(04)00218-2).

Record type: Article

Abstract

One electron reductions of oxygen and nitrite by xanthine oxidase form peroxynitrite. The nitrite and oxygen reducing activities of xanthine oxidase are regulated by oxygen with K(oxygen) 26 and 100 microM and K(nitrite) 1.0 and 1.1 mM with xanthine and NADH as donor substrates. Optimal peroxynitrite formation occurs at 70 microM oxygen with purine substrates. Kinetic parameters: V(max) approximately 50 nmol/min/mg and K(m) of 22, 36 and 70 microM for hypoxanthine, pterin and nitrite respectively. Peroxynitrite generation is inhibited by allopurinol, superoxide dismutase and diphenylene iodonium. A role for this enzyme activity can be found in the antibacterial activity of milk and circulating xanthine oxidase activity.

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More information

Published date: 2004
Keywords: xanthine oxidase, peroxynitrite, nitric oxide, superoxide

Identifiers

Local EPrints ID: 44319
URI: http://eprints.soton.ac.uk/id/eprint/44319
ISSN: 0014-5793
PURE UUID: 606f051f-94d1-46dd-bc67-cc7975d4e75c

Catalogue record

Date deposited: 23 Feb 2007
Last modified: 17 Jul 2017 15:15

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