Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase


Millar, Timothy M. (2004) Peroxynitrite formation from the simultaneous reduction of nitrite and oxygen by xanthine oxidase Febs Letters, 562, (1-3), pp. 129-133. (doi:10.1016/S0014-5793(04)00218-2).

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Description/Abstract

One electron reductions of oxygen and nitrite by xanthine oxidase form peroxynitrite. The nitrite and oxygen reducing activities of xanthine oxidase are regulated by oxygen with K(oxygen) 26 and 100 microM and K(nitrite) 1.0 and 1.1 mM with xanthine and NADH as donor substrates. Optimal peroxynitrite formation occurs at 70 microM oxygen with purine substrates. Kinetic parameters: V(max) approximately 50 nmol/min/mg and K(m) of 22, 36 and 70 microM for hypoxanthine, pterin and nitrite respectively. Peroxynitrite generation is inhibited by allopurinol, superoxide dismutase and diphenylene iodonium. A role for this enzyme activity can be found in the antibacterial activity of milk and circulating xanthine oxidase activity.

Item Type: Article
Digital Object Identifier (DOI): doi:10.1016/S0014-5793(04)00218-2
ISSNs: 0014-5793 (print)
Related URLs:
Keywords: xanthine oxidase, peroxynitrite, nitric oxide, superoxide
Subjects:
ePrint ID: 44319
Date :
Date Event
2004Published
Date Deposited: 23 Feb 2007
Last Modified: 16 Apr 2017 18:44
Further Information:Google Scholar
URI: http://eprints.soton.ac.uk/id/eprint/44319

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