The University of Southampton
University of Southampton Institutional Repository

Xanthine oxidase mediates cytokine-induced, but not hormone-induced bone resorption

Kanczler, Janos M., Millar, Timothy M., Bodamyali, Tulin, Blake, David R. and Stevens, Cliff R. (2003) Xanthine oxidase mediates cytokine-induced, but not hormone-induced bone resorption Free Radical Research, 37, (2), pp. 179-187. (doi:10.1080/1071576021000040673).

Record type: Article

Abstract

Reactive oxygen species (ROS) such as hydrogen peroxide (H2O2) have been implicated as mediators of osteoclastic bone resorption. Xanthine oxidase (XO) a ubiquitous enzyme is widely known for its production of these ROS. We therefore evaluated the potential of XO as a source of ROS in cytokine-and hormone-induced bone resorption. XO activity in rat calvarial osteoblasts was found to be significantly elevated upon stimulation by the cytokines, TNFalpha and IL-1beta. These cytokines also caused a dose related increase in bone resorption of mouse calvariae, which was significantly inhibited by catalase (10 IU/ml). Allopurinol, the competitive inhibitor of XO, also caused a dose related (1-50 microM) inhibition of TNFalpha (20 ng/ml) and (0.01-10 microM) IL-1beta (50 IU/ml)-induced bone resorption, respectively. PTH- and 1,25-(OH)2 Vitamin D3-induced bone resorption could also be inhibited by catalase (100 IU/ml) but was unaffected by allopurinol, indicating that another mediator, other than XO, is required for hormone-induced bone resorption. These results demonstrate, that modulation of the redox balance in the bone microenvironment, which contains XO, can affect the bone resorbing process. Therefore, XO may play a pivotal role in cytokine-induced bone resorption and, if manipulated appropriately, could show a therapeutic benefit in inflammatory bone disorders such as RA.

Full text not available from this repository.

More information

Published date: February 2003
Keywords: xanthine oxidase, bone resorption, cytokines, hydrogen peroxide

Identifiers

Local EPrints ID: 44322
URI: http://eprints.soton.ac.uk/id/eprint/44322
ISSN: 1071-5762
PURE UUID: f8ecb348-c231-4a11-8829-a9130ac3faea
ORCID for Janos M. Kanczler: ORCID iD orcid.org/0000-0001-7249-0414

Catalogue record

Date deposited: 23 Feb 2007
Last modified: 17 Jul 2017 15:15

Export record

Altmetrics

Contributors

Author: Janos M. Kanczler ORCID iD
Author: Tulin Bodamyali
Author: David R. Blake
Author: Cliff R. Stevens

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×