The University of Southampton
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Healthcare Workers Bioresource: Study outline and baseline characteristics of a prospective healthcare worker cohort to study immune protection and pathogenesis in COVID-19

Healthcare Workers Bioresource: Study outline and baseline characteristics of a prospective healthcare worker cohort to study immune protection and pathogenesis in COVID-19
Healthcare Workers Bioresource: Study outline and baseline characteristics of a prospective healthcare worker cohort to study immune protection and pathogenesis in COVID-19
Background: Most biomedical research has focused on sampling COVID-19 patients presenting to hospital with advanced disease, with less focus on the asymptomatic or paucisymptomatic. We established a bioresource with serial sampling of health care workers (HCWs) designed to obtain samples before and during mainly mild disease, with follow-up sampling to evaluate the quality and duration of immune memory.

Methods: We conducted a prospective observational study on HCWs from three hospital sites in London, initially at a single centre (recruited just prior to first peak community transmission in London), but then extended to multiple sites 3 weeks later (recruitment still ongoing, target n=1,000). Asymptomatic participants attending work complete a health questionnaire, and provide a nasal swab (for SARS-CoV-2 RNA by RT-PCR tests) and blood samples (mononuclear cells, serum, plasma, RNA and DNA are biobanked) at 16 weekly study visits, and at 6 and 12 months.

Results: Preliminary baseline results for the first 731 HCWs (400 single-centre, 331 multicentre extension) are presented. Mean age was 38±11 years; 67% are female, 31% nurses, 20% doctors, and 19% work in intensive care units. COVID-19-associated risk factors were: 37% black, Asian or minority ethnicities; 18% smokers; 13% obesity; 11% asthma; 7% hypertension and 2% diabetes mellitus. At baseline, 41% reported symptoms in the preceding 2 weeks. Preliminary test results from the initial cohort (n=400) are available: PCR at baseline for SARS-CoV-2 was positive in 28 of 396 (7.1%, 95% CI 4.9-10.0%) and 15 of 385 (3.9%, 2.4-6.3%) had circulating IgG antibodies.

Conclusions: This COVID-19 bioresource established just before the peak of infections in the UK will provide longitudinal assessments of incident infection and immune responses in HCWs through the natural time course of disease and convalescence. The samples and data from this bioresource are available to academic collaborators by application https://covid-consortium.com/application-for-samples/.
2398-502X
Augusto, João B.
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Augusto, João B.
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Menacho, Katia
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Seraphim, Andreas
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Jensen, Melanie
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Davies, Rhodri H.
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Captur, Gabriella
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Fontana, Marianna
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Montgomery, Hugh
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O’Brien, Ben
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Hingorani, Aroon D.
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Cutino-Moguel, Teresa
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Mcknight, Áine
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Bullock, Natalie
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Champion, Nicola
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Chan, Carmen
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Couto-Parada, Xose
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Augusto, João B., Menacho, Katia, Andiapen, Mervyn, Bowles, Ruth, Burton, Maudrian, Welch, Sophie, Bhuva, Anish N., Seraphim, Andreas, Pade, Corinna, Joy, George, Jensen, Melanie, Davies, Rhodri H., Captur, Gabriella, Fontana, Marianna, Montgomery, Hugh, O’Brien, Ben, Hingorani, Aroon D., Cutino-Moguel, Teresa, Mcknight, Áine, Abbass, Hakam, Alfarih, Mashael, Alldis, Zoe, Baca, Georgina L., Boulter, Alex, Bracken, Olivia V., Bullock, Natalie, Champion, Nicola, Chan, Carmen, Couto-Parada, Xose, Dieobi-Anene, Keenan, Feehan, Karen, Figtree, Gemma, Figtree, Melanie C., Finlay, Malcolm, Forooghi, Nasim, Gibbons, Joseph M., Griffiths, Peter, Hamblin, Matt, Howes, Lee, Itua, Ivie, Jones, Meleri, Jardim, Victor, Kapil, Vikas, Jason Lee, Wing-Yiu, Mandadapu, Vineela, Mfuko, Celina, Mitchelmore, Oliver, Palma, Susana, Patel, Kush, Petersen, Steffen E., Piniera, Brian, Raine, Rosalind, Rapala, Alicja, Richards, Amy, Sambile, Genine, Couto De Sousa, Jorge, Sugimoto, Michelle, Thornton, George D., Artico, Jessica, Zahedi, Dan, Parker, Ruth, Robathan, Mathew, Hickling, Lauren M., Ntusi, Ntobeko, Semper, Amanda, Brooks, Tim, Jones, Jessica, Tucker, Art, Veerapen, Jessry, Vijayakumar, Mohit, Wodehouse, Theresa, Wynne, Lucinda, Treibel, Thomas A., Noursadeghi, Mahdad, Manisty, Charlotte and Moon, James C. (2020) Healthcare Workers Bioresource: Study outline and baseline characteristics of a prospective healthcare worker cohort to study immune protection and pathogenesis in COVID-19. Wellcome Open Research, 5. (doi:10.12688/wellcomeopenres.16051.1).

Record type: Article

Abstract

Background: Most biomedical research has focused on sampling COVID-19 patients presenting to hospital with advanced disease, with less focus on the asymptomatic or paucisymptomatic. We established a bioresource with serial sampling of health care workers (HCWs) designed to obtain samples before and during mainly mild disease, with follow-up sampling to evaluate the quality and duration of immune memory.

Methods: We conducted a prospective observational study on HCWs from three hospital sites in London, initially at a single centre (recruited just prior to first peak community transmission in London), but then extended to multiple sites 3 weeks later (recruitment still ongoing, target n=1,000). Asymptomatic participants attending work complete a health questionnaire, and provide a nasal swab (for SARS-CoV-2 RNA by RT-PCR tests) and blood samples (mononuclear cells, serum, plasma, RNA and DNA are biobanked) at 16 weekly study visits, and at 6 and 12 months.

Results: Preliminary baseline results for the first 731 HCWs (400 single-centre, 331 multicentre extension) are presented. Mean age was 38±11 years; 67% are female, 31% nurses, 20% doctors, and 19% work in intensive care units. COVID-19-associated risk factors were: 37% black, Asian or minority ethnicities; 18% smokers; 13% obesity; 11% asthma; 7% hypertension and 2% diabetes mellitus. At baseline, 41% reported symptoms in the preceding 2 weeks. Preliminary test results from the initial cohort (n=400) are available: PCR at baseline for SARS-CoV-2 was positive in 28 of 396 (7.1%, 95% CI 4.9-10.0%) and 15 of 385 (3.9%, 2.4-6.3%) had circulating IgG antibodies.

Conclusions: This COVID-19 bioresource established just before the peak of infections in the UK will provide longitudinal assessments of incident infection and immune responses in HCWs through the natural time course of disease and convalescence. The samples and data from this bioresource are available to academic collaborators by application https://covid-consortium.com/application-for-samples/.

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Accepted/In Press date: 28 July 2020
e-pub ahead of print date: 28 July 2020

Identifiers

Local EPrints ID: 443352
URI: http://eprints.soton.ac.uk/id/eprint/443352
ISSN: 2398-502X
PURE UUID: 612df3fa-97b0-46a1-8648-88657fe468f9
ORCID for Peter Griffiths: ORCID iD orcid.org/0000-0003-2439-2857

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Date deposited: 21 Aug 2020 16:30
Last modified: 13 Dec 2021 03:05

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Contributors

Author: João B. Augusto
Author: Katia Menacho
Author: Mervyn Andiapen
Author: Ruth Bowles
Author: Maudrian Burton
Author: Sophie Welch
Author: Anish N. Bhuva
Author: Andreas Seraphim
Author: Corinna Pade
Author: George Joy
Author: Melanie Jensen
Author: Rhodri H. Davies
Author: Gabriella Captur
Author: Marianna Fontana
Author: Hugh Montgomery
Author: Ben O’Brien
Author: Aroon D. Hingorani
Author: Teresa Cutino-Moguel
Author: Áine Mcknight
Author: Hakam Abbass
Author: Mashael Alfarih
Author: Zoe Alldis
Author: Georgina L. Baca
Author: Alex Boulter
Author: Olivia V. Bracken
Author: Natalie Bullock
Author: Nicola Champion
Author: Carmen Chan
Author: Xose Couto-Parada
Author: Keenan Dieobi-Anene
Author: Karen Feehan
Author: Gemma Figtree
Author: Melanie C. Figtree
Author: Malcolm Finlay
Author: Nasim Forooghi
Author: Joseph M. Gibbons
Author: Peter Griffiths ORCID iD
Author: Matt Hamblin
Author: Lee Howes
Author: Ivie Itua
Author: Meleri Jones
Author: Victor Jardim
Author: Vikas Kapil
Author: Wing-Yiu Jason Lee
Author: Vineela Mandadapu
Author: Celina Mfuko
Author: Oliver Mitchelmore
Author: Susana Palma
Author: Kush Patel
Author: Steffen E. Petersen
Author: Brian Piniera
Author: Rosalind Raine
Author: Alicja Rapala
Author: Amy Richards
Author: Genine Sambile
Author: Jorge Couto De Sousa
Author: Michelle Sugimoto
Author: George D. Thornton
Author: Jessica Artico
Author: Dan Zahedi
Author: Ruth Parker
Author: Mathew Robathan
Author: Lauren M. Hickling
Author: Ntobeko Ntusi
Author: Amanda Semper
Author: Tim Brooks
Author: Jessica Jones
Author: Art Tucker
Author: Jessry Veerapen
Author: Mohit Vijayakumar
Author: Theresa Wodehouse
Author: Lucinda Wynne
Author: Thomas A. Treibel
Author: Mahdad Noursadeghi
Author: Charlotte Manisty
Author: James C. Moon

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