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Local photo-polymer deposition-assisted fabrication of multilayer paper-based devices

Local photo-polymer deposition-assisted fabrication of multilayer paper-based devices
Local photo-polymer deposition-assisted fabrication of multilayer paper-based devices
In this paper, we report on the use of a local acrylate-based negative photo-polymer deposition technique for the fabrication of 3D microfluidic paper-based analytical devices (3D-μPADs) where the sample flows in both the lateral and vertical directions through multiple stacked layers of porous materials. A simple and inexpensive manufacturing method was used, which is based on the local deposition of a photo-polymer (deposition speed 30 mm/s) on a porous cellulose paper substrate followed by the subsequent exposure (scanning speed 30 mm/s) to a laser source (fibre coupled continuous wave at 405 nm with maximum power of 60 mW), to stack four layers of cellulose paper and make 3D multilayer μPADs. With this technique, we provide a pathway to eliminate the limitations that other reported methods have during the fabrication of μPADs such as the need for multiple sophisticated alignments between adjoining layers and the use of additional tools to ensure adequate contact between the layers. In this study, we demonstrate the usefulness of our four-layer 3D-μPAD for simultaneous detection of three analytes, namely BSA, glucose, nitrite spiked in artificial urine and also the pH of the tested sample, through single step colorimetric assays with the limit of detection found at 0.4 mg/mL for BSA, 14.5 μg/mL for glucose and 2.5 μg/mL for nitrite. Our 3D-μPAD fabrication methodology can also be adapted in more complex analytical assays where multiple steps are needed for applications in point-of-care diagnostics.
3D Multilayer device, Diagnostics, Laser patterning method, Multiplexed detection, Paper-based device, Photo-polymer
0925-4005
Galanis, Panagiotis
4457b788-deef-4293-ab39-76f501b9529d
He, Peijun
2e303166-6aa5-4a09-b22e-440d96a54a9f
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
Iles, Alice
4c7e0b28-8a6e-442e-88ca-a06e6e1a453d
Kumar, Anto J.U.
202a7bb5-ef3b-4049-8749-3e4b91418c78
Eason, R.W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin
9de9d8ee-d394-46a5-80b7-e341c0eed0a8
Galanis, Panagiotis
4457b788-deef-4293-ab39-76f501b9529d
He, Peijun
2e303166-6aa5-4a09-b22e-440d96a54a9f
Katis, Ioannis
f92dfb8f-610d-4877-83f6-fd26a571df12
Iles, Alice
4c7e0b28-8a6e-442e-88ca-a06e6e1a453d
Kumar, Anto J.U.
202a7bb5-ef3b-4049-8749-3e4b91418c78
Eason, R.W.
e38684c3-d18c-41b9-a4aa-def67283b020
Sones, Collin
9de9d8ee-d394-46a5-80b7-e341c0eed0a8

Galanis, Panagiotis, He, Peijun, Katis, Ioannis, Iles, Alice, Kumar, Anto J.U., Eason, R.W. and Sones, Collin (2020) Local photo-polymer deposition-assisted fabrication of multilayer paper-based devices. Sensors and Actuators, B: Chemical, 322, [128574]. (doi:10.1016/j.snb.2020.128574).

Record type: Article

Abstract

In this paper, we report on the use of a local acrylate-based negative photo-polymer deposition technique for the fabrication of 3D microfluidic paper-based analytical devices (3D-μPADs) where the sample flows in both the lateral and vertical directions through multiple stacked layers of porous materials. A simple and inexpensive manufacturing method was used, which is based on the local deposition of a photo-polymer (deposition speed 30 mm/s) on a porous cellulose paper substrate followed by the subsequent exposure (scanning speed 30 mm/s) to a laser source (fibre coupled continuous wave at 405 nm with maximum power of 60 mW), to stack four layers of cellulose paper and make 3D multilayer μPADs. With this technique, we provide a pathway to eliminate the limitations that other reported methods have during the fabrication of μPADs such as the need for multiple sophisticated alignments between adjoining layers and the use of additional tools to ensure adequate contact between the layers. In this study, we demonstrate the usefulness of our four-layer 3D-μPAD for simultaneous detection of three analytes, namely BSA, glucose, nitrite spiked in artificial urine and also the pH of the tested sample, through single step colorimetric assays with the limit of detection found at 0.4 mg/mL for BSA, 14.5 μg/mL for glucose and 2.5 μg/mL for nitrite. Our 3D-μPAD fabrication methodology can also be adapted in more complex analytical assays where multiple steps are needed for applications in point-of-care diagnostics.

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Local photo-polymer deposition-assisted fabrication of multilayer paper-based devices - Accepted Manuscript
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More information

Accepted/In Press date: 8 July 2020
e-pub ahead of print date: 11 July 2020
Published date: 1 November 2020
Keywords: 3D Multilayer device, Diagnostics, Laser patterning method, Multiplexed detection, Paper-based device, Photo-polymer

Identifiers

Local EPrints ID: 443381
URI: http://eprints.soton.ac.uk/id/eprint/443381
ISSN: 0925-4005
PURE UUID: 6ba743b4-a352-4523-a7f8-75f3febcef75
ORCID for Panagiotis Galanis: ORCID iD orcid.org/0000-0002-2028-5803
ORCID for Ioannis Katis: ORCID iD orcid.org/0000-0002-2016-557X
ORCID for R.W. Eason: ORCID iD orcid.org/0000-0001-9704-2204

Catalogue record

Date deposited: 24 Aug 2020 16:30
Last modified: 15 Sep 2021 04:38

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