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Corroborating µCT and histological data to provide novel insight into the biological response to cochlear implantation at the electrode-tissue interface

Corroborating µCT and histological data to provide novel insight into the biological response to cochlear implantation at the electrode-tissue interface
Corroborating µCT and histological data to provide novel insight into the biological response to cochlear implantation at the electrode-tissue interface
Cochlear implants are the most successful neuro-prostheses. They restore hearing by replacing the function of damaged sensory cells inside the cochlea. Direct stimulation of the auditory nerve is driven through current generated at electrodes inserted into the cochlea. Despite the success of cochlear implants some individuals underperform or fail i.e. do not achieve the anticipated benefits. A significant proportion of the failures are not due to hardware or surgical factors but are likely partly due to the biological response at the electrode-tissue interface. As availability of human tissue to investigate the tissue response to cochlear implantation is limited, there is great need for effective in vivo models. We are establishing a mouse model to investigate the response at the electrode-tissue interface with the aim of understanding how the response to the implant may alter hearing performance, and how this is altered by different cochlear implants.
As part of the characterisation of the model, µCT will be used to provide 3D structural information about the cochlea pre-implantation. Optimally designed, functional, electrode arrays will be surgically implanted into mice through the round window of the cochlea. µCT scans will be captured as a method to validate surgical technique and identify tissue damage caused by electrode insertion.
The aim is to corroborate the 3D structural information with detailed cellular information from post-mortem histological analysis to gain further understanding in the biological response at the electrode-tissue interface in the rodent model.
Preliminary work determined the µCT resolution possible of a decalcified wax-embedded cochlea. Highly informative, high resolution images were obtained as shown below. Morphological information from the digital cochlea µCT sections will now be compared with tissue morphology and cellular expression information obtained from immunostaining.
Pairing high resolution, µCT images of the mouse cochlea post-implantation with in-depth histological analysis of the tissue will advance understanding of the biological response at the CI-tissue interface.
Hough, Kate
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Sanderson, Alan Peter
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Anderson, Lucy
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Katsamenis, Orestis L.
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Tourrel, Guillaume
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Verschuur, Carl
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Newman, Tracey
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Hough, Kate
4ab6ce34-81be-4bff-bdc1-bdd22bbb0ace
Sanderson, Alan Peter
79d72ecf-7722-4a39-be5f-a041ba423c2b
Anderson, Lucy
efaa3028-3a49-4ad2-9a4b-9ca4f3ccf9c2
Katsamenis, Orestis L.
8553e7c3-d860-4b7a-a883-abf6c0c4b438
Tourrel, Guillaume
b72a9281-7875-4cf7-84f6-6812521198dd
Verschuur, Carl
5e15ee1c-3a44-4dbe-ad43-ec3b50111e41
Newman, Tracey
322290cb-2e9c-445d-a047-00b1bea39a25

Hough, Kate, Sanderson, Alan Peter, Anderson, Lucy, Katsamenis, Orestis L., Tourrel, Guillaume, Verschuur, Carl and Newman, Tracey (2019) Corroborating µCT and histological data to provide novel insight into the biological response to cochlear implantation at the electrode-tissue interface. Tomography for Scientific Advancement, Avenue Campus, United Kingdom. 11 - 13 Sep 2019.

Record type: Conference or Workshop Item (Poster)

Abstract

Cochlear implants are the most successful neuro-prostheses. They restore hearing by replacing the function of damaged sensory cells inside the cochlea. Direct stimulation of the auditory nerve is driven through current generated at electrodes inserted into the cochlea. Despite the success of cochlear implants some individuals underperform or fail i.e. do not achieve the anticipated benefits. A significant proportion of the failures are not due to hardware or surgical factors but are likely partly due to the biological response at the electrode-tissue interface. As availability of human tissue to investigate the tissue response to cochlear implantation is limited, there is great need for effective in vivo models. We are establishing a mouse model to investigate the response at the electrode-tissue interface with the aim of understanding how the response to the implant may alter hearing performance, and how this is altered by different cochlear implants.
As part of the characterisation of the model, µCT will be used to provide 3D structural information about the cochlea pre-implantation. Optimally designed, functional, electrode arrays will be surgically implanted into mice through the round window of the cochlea. µCT scans will be captured as a method to validate surgical technique and identify tissue damage caused by electrode insertion.
The aim is to corroborate the 3D structural information with detailed cellular information from post-mortem histological analysis to gain further understanding in the biological response at the electrode-tissue interface in the rodent model.
Preliminary work determined the µCT resolution possible of a decalcified wax-embedded cochlea. Highly informative, high resolution images were obtained as shown below. Morphological information from the digital cochlea µCT sections will now be compared with tissue morphology and cellular expression information obtained from immunostaining.
Pairing high resolution, µCT images of the mouse cochlea post-implantation with in-depth histological analysis of the tissue will advance understanding of the biological response at the CI-tissue interface.

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Tosca Poster - Kate Hough
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More information

Published date: 11 September 2019
Venue - Dates: Tomography for Scientific Advancement, Avenue Campus, United Kingdom, 2019-09-11 - 2019-09-13

Identifiers

Local EPrints ID: 443714
URI: http://eprints.soton.ac.uk/id/eprint/443714
PURE UUID: aa4e5bff-10a0-467c-b7f5-412394a08c34
ORCID for Kate Hough: ORCID iD orcid.org/0000-0002-5160-2517
ORCID for Orestis L. Katsamenis: ORCID iD orcid.org/0000-0003-4367-4147
ORCID for Tracey Newman: ORCID iD orcid.org/0000-0002-3727-9258

Catalogue record

Date deposited: 09 Sep 2020 16:34
Last modified: 18 Feb 2021 17:32

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Contributors

Author: Kate Hough ORCID iD
Author: Alan Peter Sanderson
Author: Lucy Anderson
Author: Guillaume Tourrel
Author: Carl Verschuur
Author: Tracey Newman ORCID iD

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