Gamma-linolenic and pinolenic acids exert anti-inflammatory effects in cultured human endothelial cells through their elongation products
Gamma-linolenic and pinolenic acids exert anti-inflammatory effects in cultured human endothelial cells through their elongation products
Scope: Omega-3 fatty acids (FAs) from oily fish reduce cardiovascular disease. This may be partly due to modulation of endothelial cell (EC) inflammation. Fish stocks are declining and there is a need for sustainable alternative FAs. Gamma-linolenic acid (GLA) and pinolenic acid (PLA) are plant-derived FAs, which can fulfil this role. Methods and results: EA.hy926 cells are exposed GLA and PLA prior to stimulation with tumor necrosis factor (TNF)-α. GLA and PLA are incorporated into ECs, resulting in increases in long-chain derivatives produced by elongase 5, dihomo-gamma-linolenic acid (DGLA), and eicosatrienoic acid (ETA). Both GLA and PLA (50 µm) decrease production of soluble intercellular adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein 1 (MCP-1), and regulated on activation, normal T cell expressed and secreted (RANTES). However, decreases in these mediators are not seen after pre-treatment with GLA or PLA in elongase 5 silenced EA.hy926 cells. DGLA and ETA (10 µm) decrease EC production of sICAM-1, MCP-1, RANTES, and IL-6. All FAs reduce adhesion of THP-1 monocytes to EA.hy926 cells. Both PLA (50 µm) and ETA (10 µm) decrease NFκBp65 phosphorylation. Conclusion: These effects suggest potential for GLA, PLA and their long-chain derivatives, DGLA and ETA, as sustainable anti-inflammatory alternatives to fish-derived FAs.
endothelial cells, gamma-linolenic acid, inflammation, pinolenic acid, polyunsaturated fatty acids
Baker, Ella
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Valenzuela, Carina Alejandra
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van Dooremalen, Wies
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Martínez-Fernández, Leyre
f071fe2d-2d4a-46bc-9fa1-93d1b2cc8d97
Yaqoob, Parveen
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Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
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1 October 2020
Baker, Ella
7cd5b762-d7d7-4584-b9a7-dba555085440
Valenzuela, Carina Alejandra
1a12a9b9-6504-4392-90c5-246644b0ad5c
van Dooremalen, Wies
cf6e4596-5019-4296-984d-e2bffd9ef44a
Martínez-Fernández, Leyre
f071fe2d-2d4a-46bc-9fa1-93d1b2cc8d97
Yaqoob, Parveen
42f061f8-c05f-4c16-85ad-81cecad47a51
Miles, Elizabeth
20332899-ecdb-4214-95bc-922dde36d416
Calder, Philip
1797e54f-378e-4dcb-80a4-3e30018f07a6
Baker, Ella, Valenzuela, Carina Alejandra, van Dooremalen, Wies, Martínez-Fernández, Leyre, Yaqoob, Parveen, Miles, Elizabeth and Calder, Philip
(2020)
Gamma-linolenic and pinolenic acids exert anti-inflammatory effects in cultured human endothelial cells through their elongation products.
Molecular Nutrition & Food Research, 64 (20), [2000382].
(doi:10.1002/mnfr.202000382).
Abstract
Scope: Omega-3 fatty acids (FAs) from oily fish reduce cardiovascular disease. This may be partly due to modulation of endothelial cell (EC) inflammation. Fish stocks are declining and there is a need for sustainable alternative FAs. Gamma-linolenic acid (GLA) and pinolenic acid (PLA) are plant-derived FAs, which can fulfil this role. Methods and results: EA.hy926 cells are exposed GLA and PLA prior to stimulation with tumor necrosis factor (TNF)-α. GLA and PLA are incorporated into ECs, resulting in increases in long-chain derivatives produced by elongase 5, dihomo-gamma-linolenic acid (DGLA), and eicosatrienoic acid (ETA). Both GLA and PLA (50 µm) decrease production of soluble intercellular adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein 1 (MCP-1), and regulated on activation, normal T cell expressed and secreted (RANTES). However, decreases in these mediators are not seen after pre-treatment with GLA or PLA in elongase 5 silenced EA.hy926 cells. DGLA and ETA (10 µm) decrease EC production of sICAM-1, MCP-1, RANTES, and IL-6. All FAs reduce adhesion of THP-1 monocytes to EA.hy926 cells. Both PLA (50 µm) and ETA (10 µm) decrease NFκBp65 phosphorylation. Conclusion: These effects suggest potential for GLA, PLA and their long-chain derivatives, DGLA and ETA, as sustainable anti-inflammatory alternatives to fish-derived FAs.
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Revised manuscript
- Accepted Manuscript
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GLA PLA Figures 060420
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More information
Accepted/In Press date: 1 September 2020
e-pub ahead of print date: 8 September 2020
Published date: 1 October 2020
Additional Information:
Funding Information:
E.J.B. was supported by the Biotechnology and Biological Sciences Research Council under the Food Security Doctoral Training Programme and by the Faculty of Medicine, University of Southampton. C.A.V. was supported by Comisión Nacional de Investigación Científica y Tecnológica, Gobierno de Chile (CONICYT) through its scholarship program Becas Chile. The authors thank Magda Minnion for conducting the mass spectrometry and Stefania Del Fabbro for contributions to flow cytometry.
Publisher Copyright:
© 2020 The Authors. Published by Wiley-VCH GmbH
Keywords:
endothelial cells, gamma-linolenic acid, inflammation, pinolenic acid, polyunsaturated fatty acids
Identifiers
Local EPrints ID: 443768
URI: http://eprints.soton.ac.uk/id/eprint/443768
ISSN: 1613-4125
PURE UUID: 48d83962-2e2e-4f34-a973-f58cd25eed0d
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Date deposited: 11 Sep 2020 16:30
Last modified: 17 Mar 2024 05:52
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Contributors
Author:
Ella Baker
Author:
Carina Alejandra Valenzuela
Author:
Wies van Dooremalen
Author:
Leyre Martínez-Fernández
Author:
Parveen Yaqoob
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