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Microbiota supplementation with Bifidobacterium and Lactobacillus modifies the preterm infant gut microbiota and metabolome: An observational study

Microbiota supplementation with Bifidobacterium and Lactobacillus modifies the preterm infant gut microbiota and metabolome: An observational study
Microbiota supplementation with Bifidobacterium and Lactobacillus modifies the preterm infant gut microbiota and metabolome: An observational study
Supplementation with members of the early-life microbiota as “probiotics” is increasingly used in attempts to beneficially manipulate the preterm infant gut microbiota. We performed a large observational longitudinal study comprising two preterm groups: 101 infants orally supplemented with Bifidobacterium and Lactobacillus (Bif/Lacto) and 133 infants non-supplemented (control) matched by age, sex, and delivery method. 16S rRNA gene profiling on fecal samples (n = 592) showed a predominance of Bifidobacterium and a lower abundance of pathobionts in the Bif/Lacto group. Metabolomic analysis showed higher fecal acetate and lactate and a lower fecal pH in the Bif/Lacto group compared to the control group. Fecal acetate positively correlated with relative abundance of Bifidobacterium, consistent with the ability of the supplemented Bifidobacterium strain to metabolize human milk oligosaccharides into acetate. This study demonstrates that microbiota supplementation is associated with a Bifidobacterium-dominated preterm microbiota and gastrointestinal environment more closely resembling that of full-term infants.
Bifidobacterium, Lactobacillus, human milk oligosaccharides, metabolites, microbiota, pH, pathobionts, preterm infant, probiotic, supplementation
2666-3791
Alcon-Giner, Cristina
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Dalby, Matthew J.
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Caim, Shabhonam
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Ketskemety, Jennifer
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Shaw, Alex
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Sim, Kathleen
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Lawson, Melissa A.E.
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Kiu, Raymond
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Leclaire, Charlotte
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Chalklen, Lisa
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Kujawska, Magdalena
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Mitra, Suparna
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Fardus-Reid, Fahmina
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Belteki, Gustav
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McColl, Katherine
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Swann, Jonathan R.
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Kroll, J. Simon
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Clarke, Paul
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Hall, Lindsay J.
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Alcon-Giner, Cristina
7b30b40e-c53d-4a09-85fa-f8944258f743
Dalby, Matthew J.
a3f3562a-2741-4241-92d3-68ce7f2530da
Caim, Shabhonam
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Ketskemety, Jennifer
228c3f28-178d-409d-a8f0-bd0882d21c19
Shaw, Alex
f750243c-c8b8-46a2-97cb-b12862c14ee0
Sim, Kathleen
b21b5ad4-ae54-4e78-9c9a-d5c10e83c685
Lawson, Melissa A.E.
a59f8602-7875-4118-b338-c62eb27a15d4
Kiu, Raymond
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Leclaire, Charlotte
d95b12e0-6787-43e7-a1e1-0253c207dffb
Chalklen, Lisa
a55783c8-0937-4640-b67e-56f95cfe37df
Kujawska, Magdalena
263f36a7-8ca7-4d09-90b8-b0d356033a82
Mitra, Suparna
00e0d317-d12c-47d3-ad91-d3cee29f18ca
Fardus-Reid, Fahmina
69e50f8c-6fff-469c-b69a-792a08c8682a
Belteki, Gustav
a587b21b-b8fa-456f-8a46-2d4de0d0aec6
McColl, Katherine
811fb629-fcc1-4a65-bf51-a81da768e18d
Swann, Jonathan R.
7c11a66b-f4b8-4dbf-aa17-ad8b0561b85c
Kroll, J. Simon
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Clarke, Paul
962f7c73-1c1e-4719-bfc4-9e5e8ac1fe9c
Hall, Lindsay J.
55b94742-b975-4c9d-968f-efb33d931ae3

Alcon-Giner, Cristina, Dalby, Matthew J., Caim, Shabhonam, Ketskemety, Jennifer, Shaw, Alex, Sim, Kathleen, Lawson, Melissa A.E., Kiu, Raymond, Leclaire, Charlotte, Chalklen, Lisa, Kujawska, Magdalena, Mitra, Suparna, Fardus-Reid, Fahmina, Belteki, Gustav, McColl, Katherine, Swann, Jonathan R., Kroll, J. Simon, Clarke, Paul and Hall, Lindsay J. (2020) Microbiota supplementation with Bifidobacterium and Lactobacillus modifies the preterm infant gut microbiota and metabolome: An observational study. Cell Reports Medicine, 1 (5), [100077]. (doi:10.1016/j.xcrm.2020.100077).

Record type: Article

Abstract

Supplementation with members of the early-life microbiota as “probiotics” is increasingly used in attempts to beneficially manipulate the preterm infant gut microbiota. We performed a large observational longitudinal study comprising two preterm groups: 101 infants orally supplemented with Bifidobacterium and Lactobacillus (Bif/Lacto) and 133 infants non-supplemented (control) matched by age, sex, and delivery method. 16S rRNA gene profiling on fecal samples (n = 592) showed a predominance of Bifidobacterium and a lower abundance of pathobionts in the Bif/Lacto group. Metabolomic analysis showed higher fecal acetate and lactate and a lower fecal pH in the Bif/Lacto group compared to the control group. Fecal acetate positively correlated with relative abundance of Bifidobacterium, consistent with the ability of the supplemented Bifidobacterium strain to metabolize human milk oligosaccharides into acetate. This study demonstrates that microbiota supplementation is associated with a Bifidobacterium-dominated preterm microbiota and gastrointestinal environment more closely resembling that of full-term infants.

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More information

Accepted/In Press date: 31 July 2020
e-pub ahead of print date: 25 August 2020
Published date: 25 August 2020
Additional Information: © 2020 The Author(s).
Keywords: Bifidobacterium, Lactobacillus, human milk oligosaccharides, metabolites, microbiota, pH, pathobionts, preterm infant, probiotic, supplementation

Identifiers

Local EPrints ID: 443778
URI: http://eprints.soton.ac.uk/id/eprint/443778
ISSN: 2666-3791
PURE UUID: e8bc801f-8a6e-4dbb-8c23-544765c944df
ORCID for Jonathan R. Swann: ORCID iD orcid.org/0000-0002-6485-4529

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Date deposited: 11 Sep 2020 16:30
Last modified: 28 Apr 2022 02:29

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Contributors

Author: Cristina Alcon-Giner
Author: Matthew J. Dalby
Author: Shabhonam Caim
Author: Jennifer Ketskemety
Author: Alex Shaw
Author: Kathleen Sim
Author: Melissa A.E. Lawson
Author: Raymond Kiu
Author: Charlotte Leclaire
Author: Lisa Chalklen
Author: Magdalena Kujawska
Author: Suparna Mitra
Author: Fahmina Fardus-Reid
Author: Gustav Belteki
Author: Katherine McColl
Author: J. Simon Kroll
Author: Paul Clarke
Author: Lindsay J. Hall

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