Optimization of nitric oxide donors for investigating biofilm dispersal response in Pseudomonas aeruginosa clinical isolates
Optimization of nitric oxide donors for investigating biofilm dispersal response in Pseudomonas aeruginosa clinical isolates
Pseudomonas aeruginosa biofilms contribute heavily to chronic lung infection in cystic fibrosis patients, leading to morbidity and mortality. Nitric oxide (NO) has been shown to disperse P. aeruginosa biofilms in vitro, ex vivo and in clinical trials as a promising anti-biofilm agent. Traditional NO donors such as sodium nitroprusside (SNP) have been extensively employed in different studies. However, the dosage of SNP in different studies was not consistent, ranging from 500 nM to 500 μM. SNP is light sensitive and produces cyanide, which may lead to data misinterpretation and inaccurate predictions of dispersal responses in clinical settings. New NO donors and NO delivery methods have therefore been explored. Here we assessed 7 NO donors using P. aeruginosa PAO1 and determined that SNP and Spermine NONOate (S150) successfully reduced > 60% biomass within 24 and 2 h, respectively. While neither dosage posed toxicity towards bacterial cells, chemiluminescence assays showed that SNP only released NO upon light exposure in M9 media and S150 delivered much higher performance spontaneously. S150 was then tested on 13 different cystic fibrosis P. aeruginosa (CF-PA) isolates; most CF-PA biofilms were significantly dispersed by 250 μM S150. Our work therefore discovered a commercially available NO donor S150, which disperses CF-PA biofilms efficiently within a short period of time and without releasing cyanide, as an alternative of SNP in clinical trials in the future.
Biofilm, Chemiluminescence, Cystic fibrosis, Nitric oxide, Pseudomonas aeruginosa
8859-8869
Cai, Yuming
ec0ec21f-cb8d-4407-aed8-01da53182083
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
1 October 2020
Cai, Yuming
ec0ec21f-cb8d-4407-aed8-01da53182083
Webb, Jeremy S.
ec0a5c4e-86cc-4ae9-b390-7298f5d65f8d
Cai, Yuming and Webb, Jeremy S.
(2020)
Optimization of nitric oxide donors for investigating biofilm dispersal response in Pseudomonas aeruginosa clinical isolates.
Applied Microbiology and Biotechnology, 104 (20), .
(doi:10.1007/s00253-020-10859-7).
Abstract
Pseudomonas aeruginosa biofilms contribute heavily to chronic lung infection in cystic fibrosis patients, leading to morbidity and mortality. Nitric oxide (NO) has been shown to disperse P. aeruginosa biofilms in vitro, ex vivo and in clinical trials as a promising anti-biofilm agent. Traditional NO donors such as sodium nitroprusside (SNP) have been extensively employed in different studies. However, the dosage of SNP in different studies was not consistent, ranging from 500 nM to 500 μM. SNP is light sensitive and produces cyanide, which may lead to data misinterpretation and inaccurate predictions of dispersal responses in clinical settings. New NO donors and NO delivery methods have therefore been explored. Here we assessed 7 NO donors using P. aeruginosa PAO1 and determined that SNP and Spermine NONOate (S150) successfully reduced > 60% biomass within 24 and 2 h, respectively. While neither dosage posed toxicity towards bacterial cells, chemiluminescence assays showed that SNP only released NO upon light exposure in M9 media and S150 delivered much higher performance spontaneously. S150 was then tested on 13 different cystic fibrosis P. aeruginosa (CF-PA) isolates; most CF-PA biofilms were significantly dispersed by 250 μM S150. Our work therefore discovered a commercially available NO donor S150, which disperses CF-PA biofilms efficiently within a short period of time and without releasing cyanide, as an alternative of SNP in clinical trials in the future.
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Cai-Webb2020_Article_OptimizationOfNitricOxideDonor
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Accepted/In Press date: 23 August 2020
Published date: 1 October 2020
Additional Information:
Funding Information:
This work was supported by BBSRC and Innovate UK grant to National Biofilms Innovation Centre (NBIC) and JSW.
Publisher Copyright:
© 2020, The Author(s).
Keywords:
Biofilm, Chemiluminescence, Cystic fibrosis, Nitric oxide, Pseudomonas aeruginosa
Identifiers
Local EPrints ID: 443827
URI: http://eprints.soton.ac.uk/id/eprint/443827
ISSN: 0175-7598
PURE UUID: cc596fe6-41ae-459f-ad0e-168c67c7cbca
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Date deposited: 14 Sep 2020 16:31
Last modified: 17 Mar 2024 03:08
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Author:
Yuming Cai
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