X-ray phase tomography with near-field speckles for three-dimensional virtual histology
X-ray phase tomography with near-field speckles for three-dimensional virtual histology
High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.
1221-1227
Zdora, Marie-Christine
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Thibault, Pierre
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Kuo, Willy
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Fernandez, Vincent
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Deyhle, Hans
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Vila-Comamala, Joan
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Olbinado, Margie
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Rack, Alexander
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Lackie, Peter
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Katsamenis, Orestis
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Lawson, Matthew
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Kurtcuoglu, Vartan
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Rau, Christoph
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Pfeiffer, Franz
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Zanette, Irene
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20 September 2020
Zdora, Marie-Christine
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Thibault, Pierre
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Kuo, Willy
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Fernandez, Vincent
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Deyhle, Hans
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Vila-Comamala, Joan
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Olbinado, Margie
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Rack, Alexander
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Lackie, Peter
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Katsamenis, Orestis
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Lawson, Matthew
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Kurtcuoglu, Vartan
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Rau, Christoph
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Pfeiffer, Franz
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Zanette, Irene
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Zdora, Marie-Christine, Thibault, Pierre, Kuo, Willy, Fernandez, Vincent, Deyhle, Hans, Vila-Comamala, Joan, Olbinado, Margie, Rack, Alexander, Lackie, Peter, Katsamenis, Orestis, Lawson, Matthew, Kurtcuoglu, Vartan, Rau, Christoph, Pfeiffer, Franz and Zanette, Irene
(2020)
X-ray phase tomography with near-field speckles for three-dimensional virtual histology.
Optica, 7 (9), .
(doi:10.1364/optica.399421).
Abstract
High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.
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More information
Accepted/In Press date: 6 August 2020
e-pub ahead of print date: 11 September 2020
Published date: 20 September 2020
Additional Information:
“© 2020 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved
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Local EPrints ID: 443962
URI: http://eprints.soton.ac.uk/id/eprint/443962
ISSN: 2334-2536
PURE UUID: dc45bfc1-7310-4af3-8dc5-b98efef2aed1
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Date deposited: 18 Sep 2020 16:31
Last modified: 18 Mar 2024 03:18
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Contributors
Author:
Marie-Christine Zdora
Author:
Pierre Thibault
Author:
Willy Kuo
Author:
Vincent Fernandez
Author:
Hans Deyhle
Author:
Joan Vila-Comamala
Author:
Margie Olbinado
Author:
Alexander Rack
Author:
Matthew Lawson
Author:
Vartan Kurtcuoglu
Author:
Christoph Rau
Author:
Franz Pfeiffer
Author:
Irene Zanette
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