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X-ray phase tomography with near-field speckles for three-dimensional virtual histology

X-ray phase tomography with near-field speckles for three-dimensional virtual histology
X-ray phase tomography with near-field speckles for three-dimensional virtual histology

High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.

2334-2536
1221-1227
Zdora, Marie-Christine
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Thibault, Pierre
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Kuo, Willy
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Fernandez, Vincent
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Deyhle, Hans
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Vila-Comamala, Joan
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Olbinado, Margie
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Rack, Alexander
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Lackie, Peter
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Katsamenis, Orestis
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Lawson, Matthew
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Kurtcuoglu, Vartan
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Rau, Christoph
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Pfeiffer, Franz
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Zanette, Irene
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Zdora, Marie-Christine
a2e3b04b-aef4-42f8-9e96-4707149589fb
Thibault, Pierre
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Kuo, Willy
ce41c179-0be9-40ec-a87f-caf2f38a25c8
Fernandez, Vincent
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Deyhle, Hans
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Vila-Comamala, Joan
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Olbinado, Margie
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Rack, Alexander
5c2ef8cf-2107-49c4-ae3c-f0e29d17170b
Lackie, Peter
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Katsamenis, Orestis
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Lawson, Matthew
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Kurtcuoglu, Vartan
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Rau, Christoph
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Pfeiffer, Franz
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Zanette, Irene
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Zdora, Marie-Christine, Thibault, Pierre, Kuo, Willy, Fernandez, Vincent, Deyhle, Hans, Vila-Comamala, Joan, Olbinado, Margie, Rack, Alexander, Lackie, Peter, Katsamenis, Orestis, Lawson, Matthew, Kurtcuoglu, Vartan, Rau, Christoph, Pfeiffer, Franz and Zanette, Irene (2020) X-ray phase tomography with near-field speckles for three-dimensional virtual histology. Optica, 7 (9), 1221-1227. (doi:10.1364/optica.399421).

Record type: Article

Abstract

High-contrast, high-resolution imaging of biomedical specimens is indispensable for studying organ function and pathologies. Conventional histology, the gold standard for soft-tissue visualization, is limited by its anisotropic spatial resolution, elaborate sample preparation, and lack of quantitative image information. X-ray absorption or phase tomography have been identified as promising alternatives enabling non-destructive, distortion-free three-dimensional (3D) imaging. However, reaching sufficient contrast and resolution with a simple experimental procedure remains a major challenge. Here, we present a solution based on x-ray phase tomography through speckle-based imaging (SBI). We demonstrate on a mouse kidney that SBI delivers comprehensive 3D maps of hydrated, unstained soft tissue, revealing its microstructure and delivering quantitative tissue-density values at a density resolution of better than 2 mg/cm3 and spatial resolution of better than 8 µm. We expect that SBI virtual histology will find widespread application in biomedicine and will open up new possibilities for research and histopathology.

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More information

Accepted/In Press date: 6 August 2020
e-pub ahead of print date: 11 September 2020
Published date: 20 September 2020
Additional Information: “© 2020 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved

Identifiers

Local EPrints ID: 443962
URI: http://eprints.soton.ac.uk/id/eprint/443962
ISSN: 2334-2536
PURE UUID: dc45bfc1-7310-4af3-8dc5-b98efef2aed1
ORCID for Pierre Thibault: ORCID iD orcid.org/0000-0003-1278-8846
ORCID for Peter Lackie: ORCID iD orcid.org/0000-0001-7138-3764
ORCID for Orestis Katsamenis: ORCID iD orcid.org/0000-0003-4367-4147
ORCID for Matthew Lawson: ORCID iD orcid.org/0000-0003-0115-1698

Catalogue record

Date deposited: 18 Sep 2020 16:31
Last modified: 26 Nov 2021 03:10

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Contributors

Author: Marie-Christine Zdora
Author: Pierre Thibault ORCID iD
Author: Willy Kuo
Author: Vincent Fernandez
Author: Hans Deyhle
Author: Joan Vila-Comamala
Author: Margie Olbinado
Author: Alexander Rack
Author: Peter Lackie ORCID iD
Author: Matthew Lawson ORCID iD
Author: Vartan Kurtcuoglu
Author: Christoph Rau
Author: Franz Pfeiffer
Author: Irene Zanette

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