A cold water, ultrasonically activated stream efficiently removes proteins and prion-associated amyloid from surgical stainless steel: ultrasonic water stream for surgical decontamination
A cold water, ultrasonically activated stream efficiently removes proteins and prion-associated amyloid from surgical stainless steel: ultrasonic water stream for surgical decontamination
Background: Sterile service department decontamination procedures for surgical instruments struggle to demonstrate efficient removal of the hardiest infectious contaminants, such as prion proteins. A recently designed novel system, which uses a low pressure ultrasonically activated, cold water stream, has previously demonstrated efficient hard surface cleaning of several biological contaminants. Aim: To test the efficacy of an ultrasonically activated stream for the removal of tissue proteins, including prion-associated amyloid, from surgical stainless steel surfaces. Methods: Test surfaces were contaminated with 22L, ME7 or 263K prion-infected brain homogenates. The surfaces were treated with the ultrasonically activated water stream for contact times of 5 and 10 s. Residual proteinaceous and amyloid contamination were quantified using sensitive microscopic analysis, and immunoblotting was used to characterize the eluted prion residues before and after treatment with the ultrasonically activated stream. Findings: Efficient removal of the different prion strains from the surgical stainless steel surfaces was observed, and reduced levels of protease-susceptible and -resistant prion protein was detected in recovered supernatant. Conclusion: This study demonstrated that an ultrasonically activated stream has the potential to be a cost-effective solution to improve current decontamination practices and has the potential to reduce hospital-acquired infections.
Creutzfeldt–Jakob disease, Decontamination, Prion, Protein, Surgical, Ultrasonic cleaning
649-656
Secker, T.J.
16b0a878-984f-4272-bfaa-667c7c63023a
Leighton, Timothy
3e5262ce-1d7d-42eb-b013-fcc5c286bbae
Offin, Douglas
69c8edbf-8848-4346-941a-65b381a365c2
Birkin, Peter R.
ba466560-f27c-418d-89fc-67ea4f81d0a7
Herve, Rodolphe
9baddc65-93cf-4a18-9388-088d60572b06
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
December 2020
Secker, T.J.
16b0a878-984f-4272-bfaa-667c7c63023a
Leighton, Timothy
3e5262ce-1d7d-42eb-b013-fcc5c286bbae
Offin, Douglas
69c8edbf-8848-4346-941a-65b381a365c2
Birkin, Peter R.
ba466560-f27c-418d-89fc-67ea4f81d0a7
Herve, Rodolphe
9baddc65-93cf-4a18-9388-088d60572b06
Keevil, Charles
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Secker, T.J., Leighton, Timothy, Offin, Douglas, Birkin, Peter R., Herve, Rodolphe and Keevil, Charles
(2020)
A cold water, ultrasonically activated stream efficiently removes proteins and prion-associated amyloid from surgical stainless steel: ultrasonic water stream for surgical decontamination.
Journal of Hospital Infection, 106 (4), .
(doi:10.1016/j.jhin.2020.09.021).
Abstract
Background: Sterile service department decontamination procedures for surgical instruments struggle to demonstrate efficient removal of the hardiest infectious contaminants, such as prion proteins. A recently designed novel system, which uses a low pressure ultrasonically activated, cold water stream, has previously demonstrated efficient hard surface cleaning of several biological contaminants. Aim: To test the efficacy of an ultrasonically activated stream for the removal of tissue proteins, including prion-associated amyloid, from surgical stainless steel surfaces. Methods: Test surfaces were contaminated with 22L, ME7 or 263K prion-infected brain homogenates. The surfaces were treated with the ultrasonically activated water stream for contact times of 5 and 10 s. Residual proteinaceous and amyloid contamination were quantified using sensitive microscopic analysis, and immunoblotting was used to characterize the eluted prion residues before and after treatment with the ultrasonically activated stream. Findings: Efficient removal of the different prion strains from the surgical stainless steel surfaces was observed, and reduced levels of protease-susceptible and -resistant prion protein was detected in recovered supernatant. Conclusion: This study demonstrated that an ultrasonically activated stream has the potential to be a cost-effective solution to improve current decontamination practices and has the potential to reduce hospital-acquired infections.
Text
A cold water, ultrasonic activated stream
- Accepted Manuscript
More information
Accepted/In Press date: 11 September 2020
e-pub ahead of print date: 19 September 2020
Published date: December 2020
Additional Information:
Copyright © 2020 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.
Keywords:
Creutzfeldt–Jakob disease, Decontamination, Prion, Protein, Surgical, Ultrasonic cleaning
Identifiers
Local EPrints ID: 444080
URI: http://eprints.soton.ac.uk/id/eprint/444080
ISSN: 0195-6701
PURE UUID: 6b1e6b35-8de6-4663-be69-0448c118cdf8
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Date deposited: 24 Sep 2020 16:40
Last modified: 17 Mar 2024 05:55
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Author:
Douglas Offin
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