Chondrobags: a high throughput alginatefibronectin micromass platform for in vitro human cartilage formation
Chondrobags: a high throughput alginatefibronectin micromass platform for in vitro human cartilage formation
The maintenance and expansion of the cells required for formation of tissue-engineered cartilage has, to date, proven difficult. This is, in part, due to the initial solid phase extracellular matrix demanded by the cells inhabiting this avascular tissue. Herein, we engineer an innovative alginate-fibronectin microfluidic-based carrier construct (termed a chondrobag) equipped with solid phase presentation of growth factors that support skeletal stem cell chondrogenic differentiation while preserving human articular chondrocyte phenotype. Results demonstrate biocompatibility, cell viability, proliferation and tissue-specific differentiation for chondrogenic markers SOX9, COL2A1 and ACAN. Modulation of chondrogenic cell hypertrophy, following culture within chondrobags loaded with TGF-β1, was confirmed by down-regulation of hypertrophic genes COL10A1 and MMP13. MicroRNAs involved in the chondrogenesis process, including miR-140, miR-146b and miR-138 were observed. Results demonstrate the generation of a novel high-throughput, microfluidic-based, scalable carrier that supports human chondrogenesis with significant implications therein for cartilage repair-based therapies.
Biofabrication, Chondrogenesis, Droplet-based microfluidics, Fibronectin supplementary material for this article is available online, Hydrogels, Stem cells
Witte, Kimia
4d2805fb-50ec-424c-a3c0-ced52971ab0a
De Andres Gonzalez, Maria Del Carmen
54e87e8a-1aa2-4907-a8a0-25d0c15e5e40
Wells, Julia
2a6c8f1a-1be9-4086-a3f2-117ffcf8a7f1
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Salmerón-Sánchez, Manuel
23a5226a-67e9-4bc5-976e-6dd4f1e02044
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
October 2020
Witte, Kimia
4d2805fb-50ec-424c-a3c0-ced52971ab0a
De Andres Gonzalez, Maria Del Carmen
54e87e8a-1aa2-4907-a8a0-25d0c15e5e40
Wells, Julia
2a6c8f1a-1be9-4086-a3f2-117ffcf8a7f1
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Salmerón-Sánchez, Manuel
23a5226a-67e9-4bc5-976e-6dd4f1e02044
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Witte, Kimia, De Andres Gonzalez, Maria Del Carmen, Wells, Julia, Dalby, Matthew J., Salmerón-Sánchez, Manuel and Oreffo, Richard
(2020)
Chondrobags: a high throughput alginatefibronectin micromass platform for in vitro human cartilage formation.
Biofabrication, 12 (4), [045034].
(doi:10.1088/1758-5090/abb653).
Abstract
The maintenance and expansion of the cells required for formation of tissue-engineered cartilage has, to date, proven difficult. This is, in part, due to the initial solid phase extracellular matrix demanded by the cells inhabiting this avascular tissue. Herein, we engineer an innovative alginate-fibronectin microfluidic-based carrier construct (termed a chondrobag) equipped with solid phase presentation of growth factors that support skeletal stem cell chondrogenic differentiation while preserving human articular chondrocyte phenotype. Results demonstrate biocompatibility, cell viability, proliferation and tissue-specific differentiation for chondrogenic markers SOX9, COL2A1 and ACAN. Modulation of chondrogenic cell hypertrophy, following culture within chondrobags loaded with TGF-β1, was confirmed by down-regulation of hypertrophic genes COL10A1 and MMP13. MicroRNAs involved in the chondrogenesis process, including miR-140, miR-146b and miR-138 were observed. Results demonstrate the generation of a novel high-throughput, microfluidic-based, scalable carrier that supports human chondrogenesis with significant implications therein for cartilage repair-based therapies.
Text
Chondrobags manuscript - BioFabrication - F F Accepted version
- Accepted Manuscript
Text
Supplementary data
- Other
More information
Accepted/In Press date: 8 September 2020
e-pub ahead of print date: 8 September 2020
Published date: October 2020
Keywords:
Biofabrication, Chondrogenesis, Droplet-based microfluidics, Fibronectin supplementary material for this article is available online, Hydrogels, Stem cells
Identifiers
Local EPrints ID: 444100
URI: http://eprints.soton.ac.uk/id/eprint/444100
ISSN: 1758-5082
PURE UUID: 9d37ecc0-79e1-49d8-810a-903c7f30b111
Catalogue record
Date deposited: 25 Sep 2020 16:31
Last modified: 17 Mar 2024 05:56
Export record
Altmetrics
Contributors
Author:
Kimia Witte
Author:
Maria Del Carmen De Andres Gonzalez
Author:
Julia Wells
Author:
Matthew J. Dalby
Author:
Manuel Salmerón-Sánchez
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics