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Chondrobags: a high throughput alginatefibronectin micromass platform for in vitro human cartilage formation

Chondrobags: a high throughput alginatefibronectin micromass platform for in vitro human cartilage formation
Chondrobags: a high throughput alginatefibronectin micromass platform for in vitro human cartilage formation

The maintenance and expansion of the cells required for formation of tissue-engineered cartilage has, to date, proven difficult. This is, in part, due to the initial solid phase extracellular matrix demanded by the cells inhabiting this avascular tissue. Herein, we engineer an innovative alginate-fibronectin microfluidic-based carrier construct (termed a chondrobag) equipped with solid phase presentation of growth factors that support skeletal stem cell chondrogenic differentiation while preserving human articular chondrocyte phenotype. Results demonstrate biocompatibility, cell viability, proliferation and tissue-specific differentiation for chondrogenic markers SOX9, COL2A1 and ACAN. Modulation of chondrogenic cell hypertrophy, following culture within chondrobags loaded with TGF-β1, was confirmed by down-regulation of hypertrophic genes COL10A1 and MMP13. MicroRNAs involved in the chondrogenesis process, including miR-140, miR-146b and miR-138 were observed. Results demonstrate the generation of a novel high-throughput, microfluidic-based, scalable carrier that supports human chondrogenesis with significant implications therein for cartilage repair-based therapies.

Biofabrication, Chondrogenesis, Droplet-based microfluidics, Fibronectin supplementary material for this article is available online, Hydrogels, Stem cells
1758-5082
Witte, Kimia
4d2805fb-50ec-424c-a3c0-ced52971ab0a
De Andres Gonzalez, Maria Del Carmen
54e87e8a-1aa2-4907-a8a0-25d0c15e5e40
Wells, Julia
2a6c8f1a-1be9-4086-a3f2-117ffcf8a7f1
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Salmerón-Sánchez, Manuel
23a5226a-67e9-4bc5-976e-6dd4f1e02044
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778
Witte, Kimia
4d2805fb-50ec-424c-a3c0-ced52971ab0a
De Andres Gonzalez, Maria Del Carmen
54e87e8a-1aa2-4907-a8a0-25d0c15e5e40
Wells, Julia
2a6c8f1a-1be9-4086-a3f2-117ffcf8a7f1
Dalby, Matthew J.
25dcae6a-8289-4169-abb7-c45fff0bafdc
Salmerón-Sánchez, Manuel
23a5226a-67e9-4bc5-976e-6dd4f1e02044
Oreffo, Richard
ff9fff72-6855-4d0f-bfb2-311d0e8f3778

Witte, Kimia, De Andres Gonzalez, Maria Del Carmen, Wells, Julia, Dalby, Matthew J., Salmerón-Sánchez, Manuel and Oreffo, Richard (2020) Chondrobags: a high throughput alginatefibronectin micromass platform for in vitro human cartilage formation. Biofabrication, 12 (4), [045034]. (doi:10.1088/1758-5090/abb653).

Record type: Article

Abstract

The maintenance and expansion of the cells required for formation of tissue-engineered cartilage has, to date, proven difficult. This is, in part, due to the initial solid phase extracellular matrix demanded by the cells inhabiting this avascular tissue. Herein, we engineer an innovative alginate-fibronectin microfluidic-based carrier construct (termed a chondrobag) equipped with solid phase presentation of growth factors that support skeletal stem cell chondrogenic differentiation while preserving human articular chondrocyte phenotype. Results demonstrate biocompatibility, cell viability, proliferation and tissue-specific differentiation for chondrogenic markers SOX9, COL2A1 and ACAN. Modulation of chondrogenic cell hypertrophy, following culture within chondrobags loaded with TGF-β1, was confirmed by down-regulation of hypertrophic genes COL10A1 and MMP13. MicroRNAs involved in the chondrogenesis process, including miR-140, miR-146b and miR-138 were observed. Results demonstrate the generation of a novel high-throughput, microfluidic-based, scalable carrier that supports human chondrogenesis with significant implications therein for cartilage repair-based therapies.

Text
Chondrobags manuscript - BioFabrication - F F Accepted version - Accepted Manuscript
Restricted to Repository staff only until 8 September 2021.
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Text
Supplementary data - Other
Restricted to Repository staff only until 8 September 2021.
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More information

Accepted/In Press date: 8 September 2020
e-pub ahead of print date: 8 September 2020
Published date: October 2020
Keywords: Biofabrication, Chondrogenesis, Droplet-based microfluidics, Fibronectin supplementary material for this article is available online, Hydrogels, Stem cells

Identifiers

Local EPrints ID: 444100
URI: http://eprints.soton.ac.uk/id/eprint/444100
ISSN: 1758-5082
PURE UUID: 9d37ecc0-79e1-49d8-810a-903c7f30b111
ORCID for Julia Wells: ORCID iD orcid.org/0000-0001-8272-0236
ORCID for Richard Oreffo: ORCID iD orcid.org/0000-0001-5995-6726

Catalogue record

Date deposited: 25 Sep 2020 16:31
Last modified: 18 Feb 2021 17:17

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