The University of Southampton
University of Southampton Institutional Repository

Poly(N-isopropylacrylamide) based thin microgel films for use in cell culture applications

Poly(N-isopropylacrylamide) based thin microgel films for use in cell culture applications
Poly(N-isopropylacrylamide) based thin microgel films for use in cell culture applications
Poly(N-isopropylacrylamide) (PNIPAm) is widely used to fabricate cell sheet surfaces for cell culturing, however copolymer and interpenetrated polymer networks based on PNIPAm have been rarely explored in the context of tissue engineering. Many complex and expensive techniques have been employed to produce PNIPAm-based films for cell culturing. Among them, spin coating has demonstrated to be a rapid fabrication process of thin layers with high reproducibility and uniformity. In this study, we introduce an innovative approach to produce anchored smart thin films both thermo- and electro-responsive, with the aim to integrate them in electronic devices and better control or mimic different environments for cells in vitro. Thin films were obtained by spin coating of colloidal solutions made by PNIPAm and PAAc nanogels. Anchoring the films to the substrates was obtained through heat treatment in the presence of dithiol molecules. From analyses carried out with AFM and XPS, the final samples exhibited a flat morphology and high stability to water washing. Viability tests with cells were finally carried out to demonstrate that this approach may represent a promising route to integrate those hydrogels films in electronic platforms for cell culture applications.
Acrylic Resins/chemistry, Animals, Cell Culture Techniques/methods, Cell Line, Elasticity, Hydrogels/chemistry, Mice
2045-2322
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Buratti, Elena
dc44c884-98f0-42ae-9625-aaa4aadb2611
Huang, Ruomeng
c6187811-ef2f-4437-8333-595c0d6ac978
Tusan, Camelia G.
729621e2-3f9f-4c0a-9800-99e0afeaf673
Dinelli, Franco
af180596-abf7-493c-afb3-6f3cb1e743c3
Evans, Nicholas D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Bertoldo, Monica
3bf06933-2c40-4a61-889c-db6d5829e459
Sanzari, Ilaria
0044ac56-579f-4666-8ae1-47839b9742bf
Buratti, Elena
dc44c884-98f0-42ae-9625-aaa4aadb2611
Huang, Ruomeng
c6187811-ef2f-4437-8333-595c0d6ac978
Tusan, Camelia G.
729621e2-3f9f-4c0a-9800-99e0afeaf673
Dinelli, Franco
af180596-abf7-493c-afb3-6f3cb1e743c3
Evans, Nicholas D.
06a05c97-bfed-4abb-9244-34ec9f4b4b95
Prodromakis, Themistoklis
d58c9c10-9d25-4d22-b155-06c8437acfbf
Bertoldo, Monica
3bf06933-2c40-4a61-889c-db6d5829e459

Sanzari, Ilaria, Buratti, Elena, Huang, Ruomeng, Tusan, Camelia G., Dinelli, Franco, Evans, Nicholas D., Prodromakis, Themistoklis and Bertoldo, Monica (2020) Poly(N-isopropylacrylamide) based thin microgel films for use in cell culture applications. Scientific Reports, 10 (1), [6126]. (doi:10.1038/s41598-020-63228-9).

Record type: Article

Abstract

Poly(N-isopropylacrylamide) (PNIPAm) is widely used to fabricate cell sheet surfaces for cell culturing, however copolymer and interpenetrated polymer networks based on PNIPAm have been rarely explored in the context of tissue engineering. Many complex and expensive techniques have been employed to produce PNIPAm-based films for cell culturing. Among them, spin coating has demonstrated to be a rapid fabrication process of thin layers with high reproducibility and uniformity. In this study, we introduce an innovative approach to produce anchored smart thin films both thermo- and electro-responsive, with the aim to integrate them in electronic devices and better control or mimic different environments for cells in vitro. Thin films were obtained by spin coating of colloidal solutions made by PNIPAm and PAAc nanogels. Anchoring the films to the substrates was obtained through heat treatment in the presence of dithiol molecules. From analyses carried out with AFM and XPS, the final samples exhibited a flat morphology and high stability to water washing. Viability tests with cells were finally carried out to demonstrate that this approach may represent a promising route to integrate those hydrogels films in electronic platforms for cell culture applications.

Full text not available from this repository.

More information

Accepted/In Press date: 20 March 2020
e-pub ahead of print date: 9 April 2020
Published date: 9 April 2020
Keywords: Acrylic Resins/chemistry, Animals, Cell Culture Techniques/methods, Cell Line, Elasticity, Hydrogels/chemistry, Mice

Identifiers

Local EPrints ID: 444101
URI: http://eprints.soton.ac.uk/id/eprint/444101
ISSN: 2045-2322
PURE UUID: 8aa48a41-4ab1-4e70-8d39-d9f3015b219c
ORCID for Themistoklis Prodromakis: ORCID iD orcid.org/0000-0002-6267-6909

Catalogue record

Date deposited: 25 Sep 2020 16:31
Last modified: 18 Feb 2021 17:22

Export record

Altmetrics

Contributors

Author: Ilaria Sanzari
Author: Elena Buratti
Author: Ruomeng Huang
Author: Camelia G. Tusan
Author: Franco Dinelli
Author: Monica Bertoldo

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×