READ ME File For "Toxicity of nanoparticles on lung cells and pulmonary immune defences" Dataset DOI: 10.5258/SOTON/D1522 ReadMe Author: Artur Kirjakulov, University of Southampton This dataset supports the thesis entitled "Toxicity of nanoparticles on lung cells and pulmonary immune defences" AWARDED BY: Univeristy of Southampton DATE OF AWARD: [2020] DESCRIPTION OF THE DATA ImageJ software might be required to open certain images. This dataset contains: additional 2D images and 3D images, which could not be added into the main thesis due to certain limitations. • Figure_3_2.mp4 – video file which represents a montage of 40 individual images as a uniform rebuilt particle corresponding to Figure 3.2. A montage of 40 µm polystyrene particles stack imaged with CARS in a Z-stack mode with 1 µm step. • Figure_3_9.pptx – PowerPoint file with animated Z-stack images corresponding to Figure 3.9. Multimodal images of RAW264.7 cells exposed to 20 nm AuNPs. • Figure_3_10.pptx – PowerPoint file with animated Z-stack images corresponding to Figure 3.10. Composite images of RAW264.7 containing 20 nm AuNPs acquired with the multimodal imaging system. • Figure_3_11.zip – additional set of collected TEM images corresponding to Figure 3.11. Transmission electron microscopy image of RAW264.7 cell incubated with 20 nm AuNPs. • Figure_3_12.pptx – PowerPoint file with animated Z-stack images corresponding to Figure 3.12. 3View images of RAW264.7 cells incubated with 20 nm AuNPs. • Figure_5_20.pptx – PowerPoint animation which provides visual explanation of the concept corresponding to Figure 5.20. Illustration of AuNPs mass and total number distribution in WT/AKO and DKO mice. • Figure_5_2_and_5_3.pptx - additional set of collected TEM and SEM images corresponding to Figure 5.3. Representative TEM images of collected aerosolised 20 nm AuNPs. and Figure 5.4. Representative SEM images of collected aerosolised 20 nm AuNPs. • Figure_6_2_SEM.zip – additional set of collected SEM images corresponding to Figure 6.2. Selected electron microscopy micrographs of environmental particles. • Figure_6_2_TEM.zip – additional set of collected TEM images corresponding to Figure 6.2. Selected electron microscopy micrographs of environmental particles. Date of data collection: 2016-2020 Licence: CC-BY Related projects/Funders: Jointly funded by Faculty of Medicine and Institute of Life Sciences. Additional funded obtained from Royal Society of Chemistry Date that the file was created: September, 2020