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Automation of boron chromatographic purification for δ11B analysis of coral aragonite

Automation of boron chromatographic purification for δ11B analysis of coral aragonite
Automation of boron chromatographic purification for δ11B analysis of coral aragonite
Rationale To detect the small changes in past pH, the boron isotope ratio of coral carbonates, expressed as the δ11B value, needs to be both precise and accurate (2sd <<1‰). Boron measurements by Multi‐Collector Inductively Coupled Plasma Mass Spectrometry (MC‐ICPMS) require the boron to be carefully purified before analysis, which is time consuming, and requires specialist training. Here, we use the prepFAST‐MC method that enables the automatic extraction of B (up to 25 ng load) from a CaCO3 matrix. Methods Samples were purified using the prepFAST‐MC automated system with a ~25‐μL column of Amberlite IRA743 resin. Boron isotope measurements were performed by MC‐ICPMS. The effects of matrix load, speed of sample loading onto the column, and blank contamination were tested to evaluate the effects on the purification process. The optimised protocol was tested on various standards and samples of aragonite corals. Results The blank contribution for the approach is ~60 pg and is negligible given our sample size (<0.2% sample size). Efficiency of matrix removal is demonstrated with the addition of up to 1.6 mg of dissolved low‐B calcium carbonate to NIST SRM 951 with no impact on the accuracy of δ11B values. The Japanese Geological Survey Porites reference material JCp‐1, boric acid standard NIST SRM 951, and seawater, all processed on the prepFAST‐MC system, give δ11B values within error of literature values (δ11BJCp‐1 = 24.31 ± 0.20‰ (2sd, n = 20); δ11BNIST 951 = −0.02 ± 0.15‰ (2sd, n = 13) and δ11BSeawater = 39.50 ± 0.06‰ (2sd, n = 2)). Results obtained from the coral Siderastrea siderea purified with the prepFAST‐MC system show an average offset from the manual ion‐exchange protocols of Δδ11B = 0.01 ± 0.28‰ (2sd, n = 12). Conclusions Our study demonstrates the capacity of the prepFAST‐MC method to generate accurate and reproducible δ11B values for a range of materials, without fractionation, with efficient matrix removal and with negligible blank contribution.
0951-4198
De La Vega, Elwyn
9606fbdd-1c10-4a13-956b-434f58a37b0b
Foster, Gavin
fbaa7255-7267-4443-a55e-e2a791213022
Martinez boti, Miguel A
a86b59c1-bc83-4e0f-b236-1d211f633442
Anagnostou, Eleni
4527c274-f765-44ce-89ab-0e437aa3d870
Field, M. Paul
8b6952a8-f938-4cc8-bf8b-7606147d42ec
Kim, Minkwan
18ed9a6f-484f-4a7c-bf24-b630938c1acc
Watson, Paul
3906dc5b-c1b0-4886-be59-5e1404257963
Wilson, Paul A.
f940a9f0-fa5a-4a64-9061-f0794bfbf7c6
De La Vega, Elwyn
9606fbdd-1c10-4a13-956b-434f58a37b0b
Foster, Gavin
fbaa7255-7267-4443-a55e-e2a791213022
Martinez boti, Miguel A
a86b59c1-bc83-4e0f-b236-1d211f633442
Anagnostou, Eleni
4527c274-f765-44ce-89ab-0e437aa3d870
Field, M. Paul
8b6952a8-f938-4cc8-bf8b-7606147d42ec
Kim, Minkwan
18ed9a6f-484f-4a7c-bf24-b630938c1acc
Watson, Paul
3906dc5b-c1b0-4886-be59-5e1404257963
Wilson, Paul A.
f940a9f0-fa5a-4a64-9061-f0794bfbf7c6

De La Vega, Elwyn, Foster, Gavin, Martinez boti, Miguel A, Anagnostou, Eleni, Field, M. Paul, Kim, Minkwan, Watson, Paul and Wilson, Paul A. (2020) Automation of boron chromatographic purification for δ11B analysis of coral aragonite. Rapid Communications in Mass Spectrometry, 34 (11), [e8762]. (doi:10.1002/rcm.8762).

Record type: Article

Abstract

Rationale To detect the small changes in past pH, the boron isotope ratio of coral carbonates, expressed as the δ11B value, needs to be both precise and accurate (2sd <<1‰). Boron measurements by Multi‐Collector Inductively Coupled Plasma Mass Spectrometry (MC‐ICPMS) require the boron to be carefully purified before analysis, which is time consuming, and requires specialist training. Here, we use the prepFAST‐MC method that enables the automatic extraction of B (up to 25 ng load) from a CaCO3 matrix. Methods Samples were purified using the prepFAST‐MC automated system with a ~25‐μL column of Amberlite IRA743 resin. Boron isotope measurements were performed by MC‐ICPMS. The effects of matrix load, speed of sample loading onto the column, and blank contamination were tested to evaluate the effects on the purification process. The optimised protocol was tested on various standards and samples of aragonite corals. Results The blank contribution for the approach is ~60 pg and is negligible given our sample size (<0.2% sample size). Efficiency of matrix removal is demonstrated with the addition of up to 1.6 mg of dissolved low‐B calcium carbonate to NIST SRM 951 with no impact on the accuracy of δ11B values. The Japanese Geological Survey Porites reference material JCp‐1, boric acid standard NIST SRM 951, and seawater, all processed on the prepFAST‐MC system, give δ11B values within error of literature values (δ11BJCp‐1 = 24.31 ± 0.20‰ (2sd, n = 20); δ11BNIST 951 = −0.02 ± 0.15‰ (2sd, n = 13) and δ11BSeawater = 39.50 ± 0.06‰ (2sd, n = 2)). Results obtained from the coral Siderastrea siderea purified with the prepFAST‐MC system show an average offset from the manual ion‐exchange protocols of Δδ11B = 0.01 ± 0.28‰ (2sd, n = 12). Conclusions Our study demonstrates the capacity of the prepFAST‐MC method to generate accurate and reproducible δ11B values for a range of materials, without fractionation, with efficient matrix removal and with negligible blank contribution.

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More information

Accepted/In Press date: 17 February 2020
e-pub ahead of print date: 18 February 2020
Published date: 15 June 2020

Identifiers

Local EPrints ID: 444155
URI: http://eprints.soton.ac.uk/id/eprint/444155
ISSN: 0951-4198
PURE UUID: d3f4f979-58c6-41ff-b724-d9a81a1255e0
ORCID for Gavin Foster: ORCID iD orcid.org/0000-0003-3688-9668

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Date deposited: 29 Sep 2020 17:39
Last modified: 18 Feb 2021 17:14

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