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An extra dimension in nucleic acid sequence recognition

An extra dimension in nucleic acid sequence recognition
An extra dimension in nucleic acid sequence recognition
Watson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to nucleic acid interactions and open up many new applications. In principle the triplex approach could provide this if developed to recognize any DNA sequence. To this end modified nucleosides have been incorporated into triple-helix-forming oligonucleotides (TFOs) and used to recognize mixed sequence DNA with high selectivity and affinity at neutral pH. Continuing developments are directed towards improving TFO affinity at high pH and increasing triplex association kinetics. A number of applications of triplexes are currently being explored.
triple-helix formation, double-stranded dna, forming oligonucleotides, base-pairs, 2'-aminoethoxy-modified oligonucleotides, hybeacon(tm) probes, dual recognition, cross-links, rna, stability
0033-5835
311-320
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89
Fox, Keith R.
9da5debc-4e45-473e-ab8c-550d1104659f
Brown, Tom
a64aae36-bb30-42df-88a2-11be394e8c89

Fox, Keith R. and Brown, Tom (2005) An extra dimension in nucleic acid sequence recognition. Quarterly Reviews of Biophysics, 38 (4), 311-320. (doi:10.1017/S0033583506004197).

Record type: Article

Abstract

Watson-Crick base pairing is a natural molecular recognition process that has been exploited in molecular biology and universally adopted in many fields. An additional mode of nucleic acid sequence recognition that could be used in combination with normal base pairing would add an exta dimension to nucleic acid interactions and open up many new applications. In principle the triplex approach could provide this if developed to recognize any DNA sequence. To this end modified nucleosides have been incorporated into triple-helix-forming oligonucleotides (TFOs) and used to recognize mixed sequence DNA with high selectivity and affinity at neutral pH. Continuing developments are directed towards improving TFO affinity at high pH and increasing triplex association kinetics. A number of applications of triplexes are currently being explored.

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More information

Published date: 2005
Keywords: triple-helix formation, double-stranded dna, forming oligonucleotides, base-pairs, 2'-aminoethoxy-modified oligonucleotides, hybeacon(tm) probes, dual recognition, cross-links, rna, stability
Organisations: Biological Sciences

Identifiers

Local EPrints ID: 44440
URI: https://eprints.soton.ac.uk/id/eprint/44440
ISSN: 0033-5835
PURE UUID: dcf5baaa-3325-4ba7-b8e2-e63cde25d30b
ORCID for Keith R. Fox: ORCID iD orcid.org/0000-0002-2925-7315

Catalogue record

Date deposited: 08 Mar 2007
Last modified: 14 Mar 2019 01:56

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