Yao, Hangping, Song, Yutong, Chen, Yong, Wu, Nanping, Xu, Jialu, Sun, Chujie, Zhang, Jiaxing, Weng, Tianhao, Zhang, Zheyuan, Wu, Zhigang, Cheng, Linfang, Shi, Danrong, Lu, Xiangyun, Lei, Jianlin, Crispin, Max, Shi, Yigong, Li, Lanjuan and Li, Sai (2020) Molecular architecture of the SARS-CoV-2 virus. Cell, 183 (3), 730-738.e13. (doi:10.1016/j.cell.2020.09.018).
Abstract
SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA). Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å. Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans. The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed. Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.
More information
Identifiers
Catalogue record
Export record
Altmetrics
Contributors
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.