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Molecular architecture of the SARS-CoV-2 virus

Molecular architecture of the SARS-CoV-2 virus
Molecular architecture of the SARS-CoV-2 virus

SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA). Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å. Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans. The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed. Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.

SARS-CoV-2, coronavirus, cryo-EM, cryo-electron tomography, ribonucleoprotein, spike glycoprotein, subtomogram averaging, virus assembly, virus structure
0092-8674
730-738.e13
Yao, Hangping
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Song, Yutong
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Chen, Yong
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Wu, Nanping
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Xu, Jialu
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Sun, Chujie
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Zhang, Jiaxing
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Weng, Tianhao
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Zhang, Zheyuan
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Wu, Zhigang
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Cheng, Linfang
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Shi, Danrong
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Lu, Xiangyun
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Lei, Jianlin
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Crispin, Max
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Shi, Yigong
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Li, Lanjuan
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Li, Sai
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Yao, Hangping
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Song, Yutong
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Chen, Yong
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Wu, Nanping
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Xu, Jialu
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Sun, Chujie
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Zhang, Jiaxing
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Weng, Tianhao
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Zhang, Zheyuan
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Wu, Zhigang
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Cheng, Linfang
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Shi, Danrong
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Lu, Xiangyun
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Lei, Jianlin
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Crispin, Max
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Shi, Yigong
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Li, Lanjuan
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Li, Sai
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Yao, Hangping, Song, Yutong, Chen, Yong, Wu, Nanping, Xu, Jialu, Sun, Chujie, Zhang, Jiaxing, Weng, Tianhao, Zhang, Zheyuan, Wu, Zhigang, Cheng, Linfang, Shi, Danrong, Lu, Xiangyun, Lei, Jianlin, Crispin, Max, Shi, Yigong, Li, Lanjuan and Li, Sai (2020) Molecular architecture of the SARS-CoV-2 virus. Cell, 183 (3), 730-738.e13. (doi:10.1016/j.cell.2020.09.018).

Record type: Article

Abstract

SARS-CoV-2 is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in the structural elucidation of SARS-CoV-2 proteins, the detailed architecture of the intact virus remains to be unveiled. Here we report the molecular assembly of the authentic SARS-CoV-2 virus using cryoelectron tomography (cryo-ET) and subtomogram averaging (STA). Native structures of the S proteins in pre- and postfusion conformations were determined to average resolutions of 8.7-11 Å. Compositions of the N-linked glycans from the native spikes were analyzed by mass spectrometry, which revealed overall processing states of the native glycans highly similar to that of the recombinant glycoprotein glycans. The native conformation of the ribonucleoproteins (RNPs) and their higher-order assemblies were revealed. Overall, these characterizations revealed the architecture of the SARS-CoV-2 virus in exceptional detail and shed light on how the virus packs its ∼30-kb-long single-segmented RNA in the ∼80-nm-diameter lumen.

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abd7093 Article Content v1 - Accepted Manuscript
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More information

Accepted/In Press date: 3 September 2020
e-pub ahead of print date: 6 September 2020
Published date: 29 October 2020
Keywords: SARS-CoV-2, coronavirus, cryo-EM, cryo-electron tomography, ribonucleoprotein, spike glycoprotein, subtomogram averaging, virus assembly, virus structure

Identifiers

Local EPrints ID: 444408
URI: http://eprints.soton.ac.uk/id/eprint/444408
ISSN: 0092-8674
PURE UUID: 15542fae-9b2c-46fa-84c9-92bdd4ac2024
ORCID for Max Crispin: ORCID iD orcid.org/0000-0002-1072-2694

Catalogue record

Date deposited: 16 Oct 2020 16:32
Last modified: 13 Nov 2021 05:30

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Contributors

Author: Hangping Yao
Author: Yutong Song
Author: Yong Chen
Author: Nanping Wu
Author: Jialu Xu
Author: Chujie Sun
Author: Jiaxing Zhang
Author: Tianhao Weng
Author: Zheyuan Zhang
Author: Zhigang Wu
Author: Linfang Cheng
Author: Danrong Shi
Author: Xiangyun Lu
Author: Jianlin Lei
Author: Max Crispin ORCID iD
Author: Yigong Shi
Author: Lanjuan Li
Author: Sai Li

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