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Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium

Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium
Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium
Trichodesmium is a globally important marine microbe that provides fixed nitrogen (N) to otherwise N-limited ecosystems. In nature, nitrogen fixation is likely regulated by iron or phosphate availability, but the extent and interaction of these controls are unclear. From metaproteomics analyses using established protein biomarkers for nutrient stress, we found that iron–phosphate co-stress is the norm rather than the exception for Trichodesmium colonies in the North Atlantic Ocean. Counterintuitively, the nitrogenase enzyme was more abundant under co-stress as opposed to single nutrient stress. This is consistent with the idea that Trichodesmium has a specific physiological state during nutrient co-stress. Organic nitrogen uptake was observed and occurred simultaneously with nitrogen fixation. The quantification of the phosphate ABC transporter PstA combined with a cellular model of nutrient uptake suggested that Trichodesmium is generally confronted by the biophysical limits of membrane space and diffusion rates for iron and phosphate acquisition in the field. Colony formation may benefit nutrient acquisition from particulate and organic sources, alleviating these pressures. The results highlight that to predict the behavior of Trichodesmium, both Fe and P stress must be evaluated simultaneously.
1726-4170
2537-2551
Held, Noelle A.
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Webb, Eric A.
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Mcilvin, Matthew M.
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Hutchins, David A.
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Cohen, Natalie R.
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Moran, Dawn M.
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Kunde, Korinna
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Lohan, Maeve C.
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Mahaffey, Claire
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Woodward, E. Malcolm S.
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Saito, Mak A.
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Held, Noelle A.
23bc55c0-e3c8-4981-877d-e4703b5c18be
Webb, Eric A.
0d7ff978-20b7-407c-855a-6095364ab9a0
Mcilvin, Matthew M.
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Hutchins, David A.
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Cohen, Natalie R.
e2fed722-13c7-4099-85f2-d571caa8ff7b
Moran, Dawn M.
b4a67b6a-35b4-481a-ad2d-6d1f25ac1499
Kunde, Korinna
71069d95-19b5-45eb-a571-81103caf515d
Lohan, Maeve C.
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Mahaffey, Claire
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Woodward, E. Malcolm S.
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Saito, Mak A.
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Held, Noelle A., Webb, Eric A., Mcilvin, Matthew M., Hutchins, David A., Cohen, Natalie R., Moran, Dawn M., Kunde, Korinna, Lohan, Maeve C., Mahaffey, Claire, Woodward, E. Malcolm S. and Saito, Mak A. (2020) Co-occurrence of Fe and P stress in natural populations of the marine diazotroph Trichodesmium. Biogeosciences, 17 (9), 2537-2551. (doi:10.5194/bg-17-2537-2020).

Record type: Article

Abstract

Trichodesmium is a globally important marine microbe that provides fixed nitrogen (N) to otherwise N-limited ecosystems. In nature, nitrogen fixation is likely regulated by iron or phosphate availability, but the extent and interaction of these controls are unclear. From metaproteomics analyses using established protein biomarkers for nutrient stress, we found that iron–phosphate co-stress is the norm rather than the exception for Trichodesmium colonies in the North Atlantic Ocean. Counterintuitively, the nitrogenase enzyme was more abundant under co-stress as opposed to single nutrient stress. This is consistent with the idea that Trichodesmium has a specific physiological state during nutrient co-stress. Organic nitrogen uptake was observed and occurred simultaneously with nitrogen fixation. The quantification of the phosphate ABC transporter PstA combined with a cellular model of nutrient uptake suggested that Trichodesmium is generally confronted by the biophysical limits of membrane space and diffusion rates for iron and phosphate acquisition in the field. Colony formation may benefit nutrient acquisition from particulate and organic sources, alleviating these pressures. The results highlight that to predict the behavior of Trichodesmium, both Fe and P stress must be evaluated simultaneously.

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Accepted/In Press date: 30 March 2020
Published date: 12 May 2020

Identifiers

Local EPrints ID: 444423
URI: http://eprints.soton.ac.uk/id/eprint/444423
ISSN: 1726-4170
PURE UUID: aee0c69f-9f10-428e-a8e2-3d61b8b12b6d
ORCID for Maeve C. Lohan: ORCID iD orcid.org/0000-0002-5340-3108

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Date deposited: 19 Oct 2020 16:31
Last modified: 26 Nov 2021 03:00

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Contributors

Author: Noelle A. Held
Author: Eric A. Webb
Author: Matthew M. Mcilvin
Author: David A. Hutchins
Author: Natalie R. Cohen
Author: Dawn M. Moran
Author: Korinna Kunde
Author: Maeve C. Lohan ORCID iD
Author: Claire Mahaffey
Author: E. Malcolm S. Woodward
Author: Mak A. Saito

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