The University of Southampton
University of Southampton Institutional Repository

A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression

A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression
A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression

Barrier-to-Autointegration Factor (BAF) is a conserved nuclear envelope (NE) component that binds chromatin and helps its anchoring to the NE. Cycles of phosphorylation and dephosphorylation control BAF function. Entering mitosis, phosphorylation releases BAF from chromatin and facilitates NE-disassembly. At mitotic exit, PP2A-mediated dephosphorylation restores chromatin binding and nucleates NE-reassembly. Here, we show that in Drosophila a small fraction of BAF (cenBAF) associates with centromeres. We also find that PP4 phosphatase, which is recruited to centromeres by CENP-C, prevents phosphorylation and release of cenBAF during mitosis. cenBAF is necessary for proper centromere assembly and accurate chromosome segregation, being critical for mitosis progression. Disrupting cenBAF localization prevents PP2A inactivation in mitosis compromising global BAF phosphorylation, which in turn leads to its persistent association with chromatin, delays anaphase onset and causes NE defects. These results suggest that, together with PP4 and CENP-C, cenBAF forms a centromere-based mechanism that controls chromosome segregation and mitosis progression.

2399-3642
1-13
Torras-Llort, Mònica
36e92e44-7115-44f7-ba2e-4f25ec763777
Medina-Giró, Sònia
cf4e5ebd-40dc-409c-993b-218921619d86
Escudero-Ferruz, Paula
fcd1c3ea-dd44-4578-9ccd-0eb5222ff82f
Lipinszki, Zoltan
e405fd6d-1bee-4725-96b7-7c315f2826ed
Moreno-Moreno, Olga
84dbc09a-9fcf-4b76-b122-e36105c19b95
Karman, Zoltan
2902d4d5-1e54-4054-89d1-e8e7a48f8349
Przewloka, Marcin R.
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Azorín, Fernando
72a3955f-208d-49f0-b338-4036c052d068
Torras-Llort, Mònica
36e92e44-7115-44f7-ba2e-4f25ec763777
Medina-Giró, Sònia
cf4e5ebd-40dc-409c-993b-218921619d86
Escudero-Ferruz, Paula
fcd1c3ea-dd44-4578-9ccd-0eb5222ff82f
Lipinszki, Zoltan
e405fd6d-1bee-4725-96b7-7c315f2826ed
Moreno-Moreno, Olga
84dbc09a-9fcf-4b76-b122-e36105c19b95
Karman, Zoltan
2902d4d5-1e54-4054-89d1-e8e7a48f8349
Przewloka, Marcin R.
9b25e73c-ec15-43df-a5a4-ac9574bb20ab
Azorín, Fernando
72a3955f-208d-49f0-b338-4036c052d068

Torras-Llort, Mònica, Medina-Giró, Sònia, Escudero-Ferruz, Paula, Lipinszki, Zoltan, Moreno-Moreno, Olga, Karman, Zoltan, Przewloka, Marcin R. and Azorín, Fernando (2020) A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression. Communications Biology, 3 (1), 1-13, [454]. (doi:10.1038/s42003-020-01182-y).

Record type: Article

Abstract

Barrier-to-Autointegration Factor (BAF) is a conserved nuclear envelope (NE) component that binds chromatin and helps its anchoring to the NE. Cycles of phosphorylation and dephosphorylation control BAF function. Entering mitosis, phosphorylation releases BAF from chromatin and facilitates NE-disassembly. At mitotic exit, PP2A-mediated dephosphorylation restores chromatin binding and nucleates NE-reassembly. Here, we show that in Drosophila a small fraction of BAF (cenBAF) associates with centromeres. We also find that PP4 phosphatase, which is recruited to centromeres by CENP-C, prevents phosphorylation and release of cenBAF during mitosis. cenBAF is necessary for proper centromere assembly and accurate chromosome segregation, being critical for mitosis progression. Disrupting cenBAF localization prevents PP2A inactivation in mitosis compromising global BAF phosphorylation, which in turn leads to its persistent association with chromatin, delays anaphase onset and causes NE defects. These results suggest that, together with PP4 and CENP-C, cenBAF forms a centromere-based mechanism that controls chromosome segregation and mitosis progression.

Text
s42003-020-01182-y - Version of Record
Available under License Creative Commons Attribution.
Download (9MB)

More information

Accepted/In Press date: 30 July 2020
Published date: 19 August 2020

Identifiers

Local EPrints ID: 444492
URI: http://eprints.soton.ac.uk/id/eprint/444492
ISSN: 2399-3642
PURE UUID: 69864a42-1b8d-40bd-ac34-13b181df4d6b
ORCID for Marcin R. Przewloka: ORCID iD orcid.org/0000-0002-0329-9162

Catalogue record

Date deposited: 21 Oct 2020 16:31
Last modified: 18 Feb 2021 17:26

Export record

Altmetrics

Contributors

Author: Mònica Torras-Llort
Author: Sònia Medina-Giró
Author: Paula Escudero-Ferruz
Author: Zoltan Lipinszki
Author: Olga Moreno-Moreno
Author: Zoltan Karman
Author: Fernando Azorín

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×