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Super-resolution microscopy and FIB-SEM imaging reveal parental centriole-derived, hybrid cilium in mammalian multiciliated cells

Super-resolution microscopy and FIB-SEM imaging reveal parental centriole-derived, hybrid cilium in mammalian multiciliated cells
Super-resolution microscopy and FIB-SEM imaging reveal parental centriole-derived, hybrid cilium in mammalian multiciliated cells
Motile cilia are cellular beating machines that play a critical role in mucociliary clearance, cerebrospinal fluid movement and fertility. In the airways, the hundreds of motile cilia present on the surface of a multiciliated epithelia cell beat coordinately to protect the epithelium from bacteria, viruses and harmful particulates. During multiciliated cell differentiation, motile cilia are templated from basal bodies each extending a basal foot, an appendage linking motile cilia together to ensure coordinated beating. Here, we demonstrate that among the many motile cilia of a multiciliated cell, a hybrid cilium with structural features of both primary and motile cilia is harboured. The hybrid cilium is conserved in mammalian multiciliated cells, originates from parental centrioles and its cellular position is biased and dependent on ciliary beating. We further show that the hybrid cilium emerges independently of other motile cilia, and functions in regulating basal body alignment.
FIB-SEM, airway, appendages, basal bodies, basal foot, centrosome, cilia, electron microscopy, primary ciliary dyskinesia, quantitative imaging, super-resolution imaging
1534-5807
224-236.e6
Liu, Zhen
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Nguyen, Quynh P.H.
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Nanjundappa, Rashmi
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Delgehyr, Nathalie
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Megherbi, Alexandre
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Doherty, Regan
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Thompson, James
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Jackson, Claire
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Albulescu, Alexandra
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Heng, Yew
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Lucas, Jane
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Dell, Sharon
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Meunier, Alice
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Czymmek, Kirk
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Mahjoub, Moe R.
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Mennella, Vito
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Liu, Zhen
d09d1eb1-0e5f-4822-9e6f-741c28d13fff
Nguyen, Quynh P.H.
91934f77-429f-41b2-95c8-90bdcab5e39a
Nanjundappa, Rashmi
8a8d4590-836b-48fd-b0f2-494b1789d21d
Delgehyr, Nathalie
3251dffd-4327-4470-a1b2-414822386c46
Megherbi, Alexandre
8d0a0b91-0ed2-4ce7-aada-e662d010b63c
Doherty, Regan
da470b85-b358-4e8a-aec6-983ef87a89fc
Thompson, James
a0a1e940-d720-47de-81d7-ebcd48738239
Jackson, Claire
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Albulescu, Alexandra
7cedc7ca-e39e-492d-b21e-40d3b50957fb
Heng, Yew
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Lucas, Jane
5cb3546c-87b2-4e59-af48-402076e25313
Dell, Sharon
eb1ed7a7-943a-4077-84b9-7c4fc09f098b
Meunier, Alice
6915dc82-44df-4d62-b091-790aafbc1960
Czymmek, Kirk
56528ef6-fccc-425c-aabf-23b4b176998a
Mahjoub, Moe R.
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Mennella, Vito
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Liu, Zhen, Nguyen, Quynh P.H., Nanjundappa, Rashmi, Delgehyr, Nathalie, Megherbi, Alexandre, Doherty, Regan, Thompson, James, Jackson, Claire, Albulescu, Alexandra, Heng, Yew, Lucas, Jane, Dell, Sharon, Meunier, Alice, Czymmek, Kirk, Mahjoub, Moe R. and Mennella, Vito (2020) Super-resolution microscopy and FIB-SEM imaging reveal parental centriole-derived, hybrid cilium in mammalian multiciliated cells. Developmental Cell, 55 (2), 224-236.e6. (doi:10.1016/j.devcel.2020.09.016).

Record type: Article

Abstract

Motile cilia are cellular beating machines that play a critical role in mucociliary clearance, cerebrospinal fluid movement and fertility. In the airways, the hundreds of motile cilia present on the surface of a multiciliated epithelia cell beat coordinately to protect the epithelium from bacteria, viruses and harmful particulates. During multiciliated cell differentiation, motile cilia are templated from basal bodies each extending a basal foot, an appendage linking motile cilia together to ensure coordinated beating. Here, we demonstrate that among the many motile cilia of a multiciliated cell, a hybrid cilium with structural features of both primary and motile cilia is harboured. The hybrid cilium is conserved in mammalian multiciliated cells, originates from parental centrioles and its cellular position is biased and dependent on ciliary beating. We further show that the hybrid cilium emerges independently of other motile cilia, and functions in regulating basal body alignment.

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Accepted/In Press date: 6 October 2020
e-pub ahead of print date: 9 October 2020
Keywords: FIB-SEM, airway, appendages, basal bodies, basal foot, centrosome, cilia, electron microscopy, primary ciliary dyskinesia, quantitative imaging, super-resolution imaging

Identifiers

Local EPrints ID: 444526
URI: http://eprints.soton.ac.uk/id/eprint/444526
ISSN: 1534-5807
PURE UUID: 2fc3baba-84f5-49be-ae29-bfb70c7424d3
ORCID for Vito Mennella: ORCID iD orcid.org/0000-0002-4842-9012

Catalogue record

Date deposited: 22 Oct 2020 16:34
Last modified: 26 Nov 2021 06:35

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Contributors

Author: Zhen Liu
Author: Quynh P.H. Nguyen
Author: Rashmi Nanjundappa
Author: Nathalie Delgehyr
Author: Alexandre Megherbi
Author: Regan Doherty
Author: James Thompson
Author: Claire Jackson
Author: Alexandra Albulescu
Author: Yew Heng
Author: Jane Lucas
Author: Sharon Dell
Author: Alice Meunier
Author: Kirk Czymmek
Author: Moe R. Mahjoub
Author: Vito Mennella ORCID iD

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