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Strategies for 1H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy

Strategies for 1H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy
Strategies for 1H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy

Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic-angle spinning NMR spectroscopy. Herein, the feasibility of proton-detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected 14N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.

dynamic nuclear polarization, solid-state NMR, nitrogen-14 NMR, biomolecular NMR, amyloid
0947-6539
15852-15854
Concistre, Maria
ec95c9d4-ecb8-4c28-a464-8c3adba9e86d
Paul, Subhradip
73ab412f-f432-4ae8-a1d7-9903938f6008
Carravetta, Marina
1b12fa96-4a6a-4689-ab3b-ccc68f1d7691
Kuprov, Ilya
bb07f28a-5038-4524-8146-e3fc8344c065
Williamson, Philip
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a
Concistre, Maria
ec95c9d4-ecb8-4c28-a464-8c3adba9e86d
Paul, Subhradip
73ab412f-f432-4ae8-a1d7-9903938f6008
Carravetta, Marina
1b12fa96-4a6a-4689-ab3b-ccc68f1d7691
Kuprov, Ilya
bb07f28a-5038-4524-8146-e3fc8344c065
Williamson, Philip
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a

Concistre, Maria, Paul, Subhradip, Carravetta, Marina, Kuprov, Ilya and Williamson, Philip (2020) Strategies for 1H-Detected Dynamic Nuclear Polarization Magic-Angle Spinning NMR Spectroscopy. Chemistry - A European Journal, 26 (68), 15852-15854. (doi:10.1002/chem.202003463).

Record type: Article

Abstract

Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic-angle spinning NMR spectroscopy. Herein, the feasibility of proton-detected experiments with slow (10 kHz) magic angle spinning was demonstrated. The improvement in sensitivity permits the acquisition of indirectly detected 14N NMR spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples, in which isotope labelling is frequently intractable.

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Accepted/In Press date: 17 August 2020
e-pub ahead of print date: 22 August 2020
Published date: 4 December 2020
Additional Information: Funding Information: This work has been supported by an EPSRC grant (EP/M023664/1) to MC, IK and PTW and EPSRC funding for SP at the MAS‐DNP Facility at the University of Nottingham. Publisher Copyright: © 2020 The Authors. Published by Wiley-VCH GmbH
Keywords: dynamic nuclear polarization, solid-state NMR, nitrogen-14 NMR, biomolecular NMR, amyloid

Identifiers

Local EPrints ID: 444668
URI: http://eprints.soton.ac.uk/id/eprint/444668
ISSN: 0947-6539
PURE UUID: 207317a7-aee5-4cd2-8172-b237eba03f12
ORCID for Marina Carravetta: ORCID iD orcid.org/0000-0002-6296-2104
ORCID for Ilya Kuprov: ORCID iD orcid.org/0000-0003-0430-2682
ORCID for Philip Williamson: ORCID iD orcid.org/0000-0002-0231-8640

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Date deposited: 28 Oct 2020 18:05
Last modified: 22 Oct 2022 04:02

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Contributors

Author: Maria Concistre
Author: Subhradip Paul
Author: Ilya Kuprov ORCID iD

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