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Strategies for 1H‐detected dynamic nuclear polarisation magic‐angle spinning NMR’

Strategies for 1H‐detected dynamic nuclear polarisation magic‐angle spinning NMR’
Strategies for 1H‐detected dynamic nuclear polarisation magic‐angle spinning NMR’
Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR. Here we demonstrate the feasibility of proton‐ detected experiments with slow (10 kHz) magic angle spinning. The improvement in sensitivity permits the acquisition of indirectly detected 14N spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples where isotope labelling is frequently intractable.
dynamic nuclear polarization, solid-state NMR, nitrogen-14 NMR, biomolecular NMR, amyloid
0947-6539
Concistre, Maria
ec95c9d4-ecb8-4c28-a464-8c3adba9e86d
Paul, Subhradip
73ab412f-f432-4ae8-a1d7-9903938f6008
Carravetta, Marina
1b12fa96-4a6a-4689-ab3b-ccc68f1d7691
Kuprov, Ilya
bb07f28a-5038-4524-8146-e3fc8344c065
Williamson, Philip
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a
Concistre, Maria
ec95c9d4-ecb8-4c28-a464-8c3adba9e86d
Paul, Subhradip
73ab412f-f432-4ae8-a1d7-9903938f6008
Carravetta, Marina
1b12fa96-4a6a-4689-ab3b-ccc68f1d7691
Kuprov, Ilya
bb07f28a-5038-4524-8146-e3fc8344c065
Williamson, Philip
0b7715c6-b60e-4e95-a1b1-6afc8b9f372a

Concistre, Maria, Paul, Subhradip, Carravetta, Marina, Kuprov, Ilya and Williamson, Philip (2020) Strategies for 1H‐detected dynamic nuclear polarisation magic‐angle spinning NMR’. Chemistry - A European Journal. (doi:10.1002/chem.202003463).

Record type: Article

Abstract

Combining dynamic nuclear polarization with proton detection significantly enhances the sensitivity of magic‐angle spinning NMR. Here we demonstrate the feasibility of proton‐ detected experiments with slow (10 kHz) magic angle spinning. The improvement in sensitivity permits the acquisition of indirectly detected 14N spectra allowing biomolecular structures to be characterized without recourse to isotope labelling. This provides a new tool for the structural characterization of environmental and medical samples where isotope labelling is frequently intractable.

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Concistre2020 - Accepted Manuscript
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Accepted/In Press date: 17 August 2020
e-pub ahead of print date: 22 August 2020
Keywords: dynamic nuclear polarization, solid-state NMR, nitrogen-14 NMR, biomolecular NMR, amyloid

Identifiers

Local EPrints ID: 444668
URI: http://eprints.soton.ac.uk/id/eprint/444668
ISSN: 0947-6539
PURE UUID: 207317a7-aee5-4cd2-8172-b237eba03f12
ORCID for Marina Carravetta: ORCID iD orcid.org/0000-0002-6296-2104
ORCID for Ilya Kuprov: ORCID iD orcid.org/0000-0003-0430-2682
ORCID for Philip Williamson: ORCID iD orcid.org/0000-0002-0231-8640

Catalogue record

Date deposited: 28 Oct 2020 18:05
Last modified: 22 Aug 2021 04:01

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Contributors

Author: Maria Concistre
Author: Subhradip Paul
Author: Ilya Kuprov ORCID iD

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