Percentage area of SYTO-9 positive green fluorescent microbes (%) Before Wash After Wash SS_SL_1 1.013 0.002 SS_SL_1 0.159 0 SS_SL_1 0.049 0 SS_SL_1 0 0 SS_SL_1 0.028 0 SS_SL_1 0.116 0 SS_SL_1 0.007 0.004 SS_SL_1 0 0.023 SS_SL_1 0 0.101 SS_SL_1 0.01 0.082 SS_SL_2 0.006 0.002 SS_SL_2 0.007 0.00083 SS_SL_2 0.07 0.034 SS_SL_2 0.022 0.00028 SS_SL_2 0.111 0 SS_SL_2 0.014 0 SS_SL_2 0.063 0.009 SS_SL_2 0.007 0 SS_SL_2 0.069 0.009 SS_SL_2 0 0 SS_SL_3 0.007 0 SS_SL_3 0.042 0.001 SS_SL_3 0.015 0.021 SS_SL_3 0.047 0 SS_SL_3 0.245 0 SS_SL_3 0.03 0 SS_SL_3 0.022 0 SS_SL_3 0.269 0 SS_SL_3 0.096 0.03 SS_SL_3 0.009 0.056 SS_SL_4 0.025 0 SS_SL_4 0 0 SS_SL_4 2.706 0 SS_SL_4 1.334 0.339 SS_SL_4 7.958 2.905 SS_SL_4 8.768 0.001 SS_SL_4 0.099 0 SS_SL_4 0.057 0 SS_SL_4 0.092 0.001 SS_SL_4 0.169 0 SS_SL_5 0.04 0.343 SS_SL_5 0.12 0.031 SS_SL_5 0.234 0.312 SS_SL_5 0.161 1.025 SS_SL_5 0.693 1.107 SS_SL_5 0.957 0.237 SS_SL_5 0.555 0.091 SS_SL_5 0.002 0.025 SS_SL_5 0.036 0.0006 SS_SL_5 0.015 0.008 Percentage area of SYTO-9 positive green fluorescent microbes (%) Before Wash After Wash SS_GL_1 0.001 0 SS_GL_1 0.494 0.088 SS_GL_1 0.038 0.038 SS_GL_1 2.402 0.02 SS_GL_1 3.607 0.001 SS_GL_1 5.178 0 SS_GL_1 0 0 SS_GL_1 7.856 0.045 SS_GL_1 7.638 0.08 SS_GL_1 0.626 0.015 SS_GL_2 0.0005 0 SS_GL_2 0 0 SS_GL_2 0 0 SS_GL_2 0.0008 0 SS_GL_2 0.041 0 SS_GL_2 0.238 0 SS_GL_2 0.002 0 SS_GL_2 0.007 0.001 SS_GL_2 0.203 0 SS_GL_2 0.337 0.067 SS_GL_3 0.7 0.062 SS_GL_3 0.214 0 SS_GL_3 0.119 0 SS_GL_3 0.0008 0.031 SS_GL_3 0.038 0.145 SS_GL_3 0.122 0 SS_GL_3 3.734 0 SS_GL_3 1.277 0.01 SS_GL_3 0.609 0.044 SS_GL_3 0.0002 0.023 SS_GL_4 0 0 SS_GL_4 0.057 0.004 SS_GL_4 0 0.005 SS_GL_4 0 0 SS_GL_4 0.23 0.021 SS_GL_4 0.042 0 SS_GL_4 0.038 0.0003 SS_GL_4 0.011 0.001 SS_GL_4 0.078 0.016 SS_GL_4 0.028 0.063 SS_GL_5 0.009 0.006 SS_GL_5 0.0008 0 SS_GL_5 0 0 SS_GL_5 0 0.0006 SS_GL_5 0 0 SS_GL_5 0.112 0 SS_GL_5 0.395 0 SS_GL_5 0.296 0 SS_GL_5 1.315 0.105 SS_GL_5 0.094 0 Percentage area of SYTO-9 positive green fluorescent microbes (%) Before Wash After Wash WW_SL_1 0 0.148 WW_SL_1 0 0.035 WW_SL_1 0.09 0.019 WW_SL_1 0.001 0.008 WW_SL_1 0 0 WW_SL_1 0.00028 0.017 WW_SL_1 0.756 0.00028 WW_SL_1 0.156 0.122 WW_SL_1 0 0 WW_SL_1 0 0 WW_SL_2 0.475 0.039 WW_SL_2 3.121 0.441 WW_SL_2 0.752 0.267 WW_SL_2 0.18 2.997 WW_SL_2 0.109 1.742 WW_SL_2 0.191 0.373 WW_SL_2 1.914 0.225 WW_SL_2 16.968 0.07 WW_SL_2 0.328 0.216 WW_SL_2 0 0.679 WW_SL_3 0.059 0.1 WW_SL_3 0.169 0.135 WW_SL_3 0.537 0.161 WW_SL_3 1.122 1.979 WW_SL_3 0.255 0.351 WW_SL_3 0 0.0003 WW_SL_3 0.037 0.0003 WW_SL_3 0.074 0.027 WW_SL_3 0.83 0.229 WW_SL_3 0.023 0.747 WW_SL_4 0.019 0.0008 WW_SL_4 0.048 0.038 WW_SL_4 0.116 0.004 WW_SL_4 0.04 0.019 WW_SL_4 0.015 0 WW_SL_4 0.16 0.029 WW_SL_4 0.005 0.042 WW_SL_4 0.028 0.0003 WW_SL_4 0.006 0 WW_SL_4 0.413 0.012 WW_SL_5 0.04 0.343 WW_SL_5 0.012 0.031 WW_SL_5 0.234 0.312 WW_SL_5 0.161 1.025 WW_SL_5 0.693 1.107 WW_SL_5 0.957 0.237 WW_SL_5 0.555 0.091 WW_SL_5 0.002 0.025 WW_SL_5 0.036 0.0006 WW_SL_5 0.015 0.008 Percentage area of SYTO-9 positive green fluorescent microbes (%) Before Wash After Wash WW_GL_1 1.858 0.43 WW_GL_1 0.0004 0.003 WW_GL_1 1.344 3.748 WW_GL_1 0 0.074 WW_GL_1 0.23 0.002 WW_GL_1 0.079 0.182 WW_GL_1 1.162 0.43 WW_GL_1 1.305 0.574 WW_GL_1 0.111 0.365 WW_GL_1 0.055 0.86 WW_GL_2 9.462 1.6 WW_GL_2 7.382 8.119 WW_GL_2 21.782 4.201 WW_GL_2 5.924 0.79 WW_GL_2 2.148 3.595 WW_GL_2 12.243 1.945 WW_GL_2 0.91 15.996 WW_GL_2 0.054 1.234 WW_GL_2 0.299 1.877 WW_GL_2 12.125 0.023 WW_GL_3 0.072 0.389 WW_GL_3 0 0.453 WW_GL_3 0 0.954 WW_GL_3 0.135 0.056 WW_GL_3 0.007 0.0003 WW_GL_3 0.002 0 WW_GL_3 0.003 0.063 WW_GL_3 0.8 0 WW_GL_3 0.765 0.305 WW_GL_3 0.39 0.104 WW_GL_4 0.572 6.914 WW_GL_4 1.087 2.103 WW_GL_4 0 3.491 WW_GL_4 1.938 5.015 WW_GL_4 7.974 11.764 WW_GL_4 1.333 6.875 WW_GL_4 5.761 3.208 WW_GL_4 5.113 1.845 WW_GL_4 8.618 0.625 WW_GL_4 0.66 1.587 WW_GL_5 0.233 0.0003 WW_GL_5 0.005 0.078 WW_GL_5 0.001 1.62 WW_GL_5 1.593 0.012 WW_GL_5 0.343 0.003 WW_GL_5 0.497 0 WW_GL_5 0.0003 0 WW_GL_5 0.283 0 WW_GL_5 0.285 0.0003 WW_GL_5 0.028 0.295