The University of Southampton
University of Southampton Institutional Repository

A fast impedance-based antimicrobial susceptibility test

A fast impedance-based antimicrobial susceptibility test
A fast impedance-based antimicrobial susceptibility test

There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10 5 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

2041-1723
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Paton, Teagan
a1e4fea0-a444-48a5-b56a-fa7b6c643244
Mulroney, Kieran
a2cee7c4-3593-4ab4-9446-efb5af57671b
Inglis, Timothy J.J.
b8bcd9dc-5c5f-4264-af79-1a78f1034d78
Sutton, J. Mark
b6a22e5d-9ba1-454f-8153-8c5e26346300
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Paton, Teagan
a1e4fea0-a444-48a5-b56a-fa7b6c643244
Mulroney, Kieran
a2cee7c4-3593-4ab4-9446-efb5af57671b
Inglis, Timothy J.J.
b8bcd9dc-5c5f-4264-af79-1a78f1034d78
Sutton, J. Mark
b6a22e5d-9ba1-454f-8153-8c5e26346300
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Spencer, Daniel, Paton, Teagan, Mulroney, Kieran, Inglis, Timothy J.J., Sutton, J. Mark and Morgan, Hywel (2020) A fast impedance-based antimicrobial susceptibility test. Nature Communications, 11 (5328), [5328]. (doi:10.1038/s41467-020-18902-x).

Record type: Article

Abstract

There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10 5 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

Text
Accepted manuscript - Version of Record
Available under License Creative Commons Attribution.
Download (1MB)

More information

Accepted/In Press date: 17 September 2020
Published date: 21 October 2020

Identifiers

Local EPrints ID: 445048
URI: http://eprints.soton.ac.uk/id/eprint/445048
ISSN: 2041-1723
PURE UUID: 7edf199b-e477-4c29-a539-c9f84215477a
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 18 Nov 2020 17:31
Last modified: 06 Jun 2024 01:42

Export record

Altmetrics

Contributors

Author: Daniel Spencer
Author: Teagan Paton
Author: Kieran Mulroney
Author: Timothy J.J. Inglis
Author: J. Mark Sutton
Author: Hywel Morgan ORCID iD

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×