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A fast impedance-based antimicrobial susceptibility test

A fast impedance-based antimicrobial susceptibility test
A fast impedance-based antimicrobial susceptibility test

There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10 5 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

2041-1723
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Paton, Teagan
a1e4fea0-a444-48a5-b56a-fa7b6c643244
Mulroney, Kieran
a2cee7c4-3593-4ab4-9446-efb5af57671b
Inglis, Timothy J.J.
b8bcd9dc-5c5f-4264-af79-1a78f1034d78
Sutton, J. Mark
b6a22e5d-9ba1-454f-8153-8c5e26346300
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Spencer, Daniel
4affe9f6-353a-4507-8066-0180b8dc9eaf
Paton, Teagan
a1e4fea0-a444-48a5-b56a-fa7b6c643244
Mulroney, Kieran
a2cee7c4-3593-4ab4-9446-efb5af57671b
Inglis, Timothy J.J.
b8bcd9dc-5c5f-4264-af79-1a78f1034d78
Sutton, J. Mark
b6a22e5d-9ba1-454f-8153-8c5e26346300
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174

Spencer, Daniel, Paton, Teagan, Mulroney, Kieran, Inglis, Timothy J.J., Sutton, J. Mark and Morgan, Hywel (2020) A fast impedance-based antimicrobial susceptibility test. Nature Communications, 11 (5328), [5328]. (doi:10.1038/s41467-020-18902-x).

Record type: Article

Abstract

There is an urgent need to develop simple and fast antimicrobial susceptibility tests (ASTs) that allow informed prescribing of antibiotics. Here, we describe a label-free AST that can deliver results within an hour, using an actively dividing culture as starting material. The bacteria are incubated in the presence of an antibiotic for 30 min, and then approximately 10 5 cells are analysed one-by-one with microfluidic impedance cytometry for 2–3 min. The measured electrical characteristics reflect the phenotypic response of the bacteria to the mode of action of a particular antibiotic, in a 30-minute incubation window. The results are consistent with those obtained by classical broth microdilution assays for a range of antibiotics and bacterial species.

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Accepted/In Press date: 17 September 2020
Published date: 21 October 2020

Identifiers

Local EPrints ID: 445048
URI: http://eprints.soton.ac.uk/id/eprint/445048
ISSN: 2041-1723
PURE UUID: 7edf199b-e477-4c29-a539-c9f84215477a
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

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Date deposited: 18 Nov 2020 17:31
Last modified: 18 Feb 2021 17:01

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Contributors

Author: Daniel Spencer
Author: Teagan Paton
Author: Kieran Mulroney
Author: Timothy J.J. Inglis
Author: J. Mark Sutton
Author: Hywel Morgan ORCID iD

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